Intracellular Staining by Flow Cytometry
|Detection of Somatostatin R2/SSTR2 in MDA‑MB‑231 Human Cell Line by Flow Cytometry. MDA‑MB‑231 human breast cancer cell line was stained with Human Somatostatin R2/SSTR2 Monoclonal Antibody (Catalog # MAB4224, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.|
|Somatostatin R2/SSTR2 in MDA-MB-231 Human Cell Line. Somatostatin R2/SSTR2 was detected in immersion fixed MDA-MB-231 human breast cancer cell line using 10 µg/mL Human Somatostatin R2/SSTR2 Monoclonal Antibody (Catalog # MAB4224) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (brown; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.|
Somatostatin R2/SSTR2 in Human Brain. Somatostatin R2/|
SSTR2 was detected in immersion fixed paraffin-embedded sections of human brain (cingulate cortex) using 3 µg/mL Human Somatostatin R2/SSTR2 Monoclonal Antibody (Catalog # MAB4224) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Somatostatin Receptor 2 (SSTR2) is one of five 7-transmembrane G-protein-coupled receptors for somatostatins 14 and 28. Human SSTR2 shares 84% aa identity with mouse SSTR2 within the extracellular domains. Isoform B (357 aa) has an alternate C-terminal cytoplasmic region that is 12 aa shorter than that of isoform A (369 aa, reported as 93 kDa). Both are expressed in brain, stomach, intestinal epithelia, pancreatic islets and kidney tubules. Isoform B is also expressed in parotid, thyroid and bronchial glands.
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