Human STRA6 Alexa Fluor® 647-conjugated Antibody

Catalog # Availability Size / Price Qty
IC5904R-100UG
Human STRA6 Alexa Fluor® 647-conjugated Antibody in Intracellular Staining by Flow Cytometry
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Human STRA6 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human STRA6 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 496613
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human STRA6
Met1-Pro667
Accession # AAH25256
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of STRA6 in HT‑29 Human Cell Line by Flow Cytometry. View Larger

Detection of STRA6 in HT‑29 Human Cell Line by Flow Cytometry. HT‑29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human STRA6 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # IC5904R, filled histogram) or isotype control antibody (Catalog # IC002R, open histogram). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin. View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: STRA6

Stimulated by retinoic acid gene 6 protein homolog (STRA6) is a 74 kDa broadly expressed transmembrane protein that does not belong to any particular family of proteins. Human STRA6 is 667 amino acids (aa) in length. It contains nine transmembrane regions and one potential N-linked glycosylation site in the first extracellular domain. In addition, there are three splicing variants producing four isoforms. There is no signal sequence. Human STRA6 is 80% identical to bovine STRA6 and 74% identical to mouse and rat STRA6. STRA6 may act as a high-affinity cell-surface receptor for the complex retinol-retinol binding protein (RBP/RBP4). Defects in STRA6 are the cause of Spear syndrome and Matthew-Wood syndrome.

Long Name
Stimulated by Retinoic Acid Gene 6 Protein Homolog
Entrez Gene IDs
64220 (Human); 20897 (Mouse)
Alternate Names
FLJ12541; MCOPS9; stimulated by retinoic acid gene 6 homolog (mouse); stimulated by retinoic acid gene 6 protein homolog; STRA6

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