Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Intracellular Staining by Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 496613
View all STRA6 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human STRA6
Met1-Pro667
Accession # AAH25256

Specificity

Detects human STRA6 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human STRA6 Antibody

Detection of STRA6 antibody in HT‑29 Human Cell Line antibody by Flow Cytometry.

Detection of STRA6 in HT‑29 Human Cell Line by Flow Cytometry.

HT-29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human STRA6 Monoclonal Antibody (Catalog # MAB5904, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
STRA6 antibody in Raji Human Cell Line by Immunocytochemistry (ICC).

STRA6 in Raji Human Cell Line.

STRA6 was detected in immersion fixed Raji human Burkitt's lymphoma cell line using Mouse Anti-Human STRA6 Monoclonal Antibody (Catalog # MAB5904) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human STRA6 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HT-29 human colon adenocarcinoma cell line and Raji human Burkitt's lymphoma cell line

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample: HT‑29 human colon adenocarcinoma cell line fixed with paraformaldehyde and permeabilized with saponin

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: STRA6

Stimulated by retinoic acid gene 6 protein homolog (STRA6) is a 74 kDa broadly expressed transmembrane protein that does not belong to any particular family of proteins. Human STRA6 is 667 amino acids (aa) in length. It contains nine transmembrane regions and one potential N-linked glycosylation site in the first extracellular domain. In addition, there are three splicing variants producing four isoforms. There is no signal sequence. Human STRA6 is 80% identical to bovine STRA6 and 74% identical to mouse and rat STRA6. STRA6 may act as a high-affinity cell-surface receptor for the complex retinol-retinol binding protein (RBP/RBP4). Defects in STRA6 are the cause of Spear syndrome and Matthew-Wood syndrome.

Long Name

Stimulated by Retinoic Acid Gene 6 Protein Homolog

Alternate Names

MCOPS9

Entrez Gene IDs

64220 (Human); 20897 (Mouse)

Gene Symbol

STRA6

UniProt

AAH25256

Additional STRA6 Products

Product Documents for Human STRA6 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human STRA6 Antibody

For research use only

Related Research Areas

Glucose Homeostasis
Obesity

Citations for Human STRA6 Antibody

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Protocols

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