Human sTransferrin Receptor/sTfR Quantikine ELISA, RUO

R&D Systems | Catalog # DTFRU0

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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Serum (25 µL), EDTA Plasma (50 µL), Heparin Plasma (25 µL)

Sensitivity

0.064 nmol/L

Assay Range

1.25-40 nmol/L (Serum, EDTA Plasma, Heparin Plasma)
View all TfR (Transferrin R) ELISA Kits »

Product Summary for Human sTransferrin Receptor/sTfR Quantikine ELISA, RUO

The Quantikine Human sTfR Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human sTfR in serum and plasma. Results obtained using natural human sTfR showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human sTfR.

Product Specifications

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Specificity

This assay recognizes natural human sTfR. 

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.

EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (nmol/L) 4.82 18.4 28.8 5.02 19.8 29.5
Standard Deviation 0.115 0.375 0.791 0.202 1.31 2.56
CV% 2.4 2.0 2.7 4.0 6.6 8.7

Recovery for Human sTransferrin Receptor/sTfR Quantikine ELISA, RUO

The recovery of human sTfR spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 107 98-114
Heparin Plasma (n=4) 107 96-119
Serum (n=4) 109 94-116

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of human sTfR in various matrices were diluted with the calibrator diluent to produce samples with values within the dynamic range of the assay.

Scientific Data Images for Human sTransferrin Receptor/sTfR Quantikine ELISA, RUO

Human sTfR Quantikine ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TfR (Transferrin R)

Transferrin Receptor (TfR, TfR-1, CD71) is a transmembrane glycoprotein expressed on erythroid progenitors, muscle cells and proliferating cells as a disulfide-linked homodimer. As the major mediator of cellular iron uptake, it binds and internalizes diferric transferrin, allowing iron release at the low pH of the endosome. Proteolytic shedding of TfR into the circulation is enhanced by iron deficiency. The hereditary hemochromatosis protein HFE competes with diferric transferrin for binding to TfR and targets TfR for degradation rather than recycling. Cancer cells can express up to 5-fold more TfR than quiescent cells in the surrounding tissue. TfR carries out ferritin-independent functions in T cell development, immunological synapse formation and Galectin-3-mediated cell death, and it serves as a cell entry receptor for New World hemorrhagic fever arenaviruses.

Long Name

Transferrin Receptor

Alternate Names

CD71, TfR (TransferrinR), TFR1, TFRC, TRFR

Entrez Gene IDs

7037 (Human); 22042 (Mouse); 64678 (Rat)

Gene Symbol

TFRC

Additional TfR (Transferrin R) Products

Product Documents for Human sTransferrin Receptor/sTfR Quantikine ELISA, RUO

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human sTransferrin Receptor/sTfR Quantikine ELISA, RUO

For research use only

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