Human STRO‑1 Antibody

R&D Systems | Catalog # MAB1038

R&D Systems
Discontinued Product
MAB1038 has been discontinued. View all STRO-1 products.

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat, Porcine, Canine, Deer, Feline

Applications

Validated:

Immunocytochemistry

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Cell Selection, MACS

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Clone # STRO-1
View all STRO-1 Primary Antibodies »

Product Specifications

Immunogen

Human CD34+ bone marrow cells

Specificity

Detects human STRO-1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Scientific Data Images for Human STRO‑1 Antibody

STRO-1 antibody in Human MG-63 Cell Line by Immunocytochemistry (ICC).

STRO‑1 in Human MG‑63 Cell Line.

STRO-1 was detected in immersion fixed MG-63 human osteosarcoma cell line using Human STRO-1 Monoclonal Antibody (Catalog # MAB1038) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgM Secondary Antibody (red; NL019), and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human STRO‑1 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed human long term bone marrow culture and immersion fixed embryonic mouse parietal endoderm cells (E10.5)

Reviewed Applications

Read 9 reviews rated 4.2 using MAB1038 in the following applications:

Formulation, Preparation, and Storage

Purification

IgM-specific Affinity-purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: STRO-1

STRO-1 is a cell surface protein expressed by bone marrow stromal cells and erythroid precursors. The frequency of colony forming units-fibroblasts (CFU-F) was enriched 100-fold in the STRO-1+/Glycophorin A- population from bone marrow cells (1). The subset of marrow cells that expresses the STRO-1 antigen is capable of differentiating into multiple mesenchymal lineages including hematopoiesis-supportive stromal cells with a vascular smooth muscle-like phenotype, adipocytes, osteoblasts, and chondrocytes (2).

References

  1. Simmons, P.J. and B. Torok-Storb (1991) Blood 78:55.
  2. Dennis, J.E. et al. (2002) Cells Tissues Organs 170:73.

Long Name

A Stromal Cell Precursor Surface Antigen

Alternate Names

STRO1

Additional STRO-1 Products

Product Documents for Human STRO‑1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human STRO‑1 Antibody

For research use only

Citations for Human STRO‑1 Antibody

Customer Reviews for Human STRO‑1 Antibody (9)

4.2 out of 5
9 Customer Ratings
5 Stars
33%
4 Stars
56%
3 Stars
11%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 5 of 9 reviews Showing All
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  • Human STRO-1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Human dental pulp cells
    Species: Human
    Verified Customer | Posted 07/20/2021
    Human STRO‑1 Antibody MAB1038
  • Human STRO-1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Stem cells
    Species: Human
    Verified Customer | Posted 07/02/2021
    Human STRO‑1 Antibody MAB1038
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: See PMID 23603337
    Species: Human
    Verified Customer | Posted 02/03/2015
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 23312853
    Species: Human
    Verified Customer | Posted 02/03/2015
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: See PMID 23072705
    Species: Human
    Verified Customer | Posted 02/03/2015
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID 23067388
    Species: Human
    Verified Customer | Posted 02/03/2015
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID 22226689
    Species: Human
    Verified Customer | Posted 02/03/2015
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID 21550429
    Species: Human
    Verified Customer | Posted 02/03/2015
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID 20682625
    Species: Human
    Verified Customer | Posted 02/03/2015

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