Human STRO-1 Antibody (STRO-1)

R&D Systems | Catalog # MAB1038

R&D Systems
Discontinued Product
MAB1038 has been discontinued. View all STRO-1 products.

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat, Porcine, Canine, Deer, Feline

Applications

Validated:

Immunocytochemistry

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Cell Selection, MACS

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Clone # STRO-1
View all STRO-1 Primary Antibodies »

Product Specifications

Immunogen

Human CD34+ bone marrow cells

Specificity

Detects human STRO-1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Scientific Data Images for Human STRO-1 Antibody (STRO-1)

STRO-1 antibody in Human MG-63 Cell Line by Immunocytochemistry (ICC).

STRO‑1 in Human MG‑63 Cell Line.

STRO-1 was detected in immersion fixed MG-63 human osteosarcoma cell line using Human STRO-1 Monoclonal Antibody (Catalog # MAB1038) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgM Secondary Antibody (red; NL019), and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human STRO-1 Antibody (STRO-1)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed human long term bone marrow culture and immersion fixed embryonic mouse parietal endoderm cells (E10.5)

Reviewed Applications

Read 9 reviews rated 4.2 using MAB1038 in the following applications:

Formulation, Preparation, and Storage

Purification

IgM-specific Affinity-purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: STRO-1

STRO-1 is a cell surface protein expressed by bone marrow stromal cells and erythroid precursors. The frequency of colony forming units-fibroblasts (CFU-F) was enriched 100-fold in the STRO-1+/Glycophorin A- population from bone marrow cells (1). The subset of marrow cells that expresses the STRO-1 antigen is capable of differentiating into multiple mesenchymal lineages including hematopoiesis-supportive stromal cells with a vascular smooth muscle-like phenotype, adipocytes, osteoblasts, and chondrocytes (2).

References

  1. Simmons, P.J. and B. Torok-Storb (1991) Blood 78:55.
  2. Dennis, J.E. et al. (2002) Cells Tissues Organs 170:73.

Long Name

A Stromal Cell Precursor Surface Antigen

Alternate Names

STRO1

Additional STRO-1 Products

Product Documents for Human STRO-1 Antibody (STRO-1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human STRO-1 Antibody (STRO-1)

For research use only

Citations for Human STRO-1 Antibody (STRO-1)

Customer Reviews for Human STRO-1 Antibody (STRO-1) (9)

4.2 out of 5
9 Customer Ratings
5 Stars
33%
4 Stars
56%
3 Stars
11%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 5 of 9 reviews Showing All
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  • Human STRO-1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Human dental pulp cells
    Species: Human
    Verified Customer | Posted 07/20/2021
    Human STRO-1 Antibody (STRO-1) MAB1038
  • Human STRO-1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Stem cells
    Species: Human
    Verified Customer | Posted 07/02/2021
    Human STRO-1 Antibody (STRO-1) MAB1038
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: See PMID 23603337
    Species: Human
    Verified Customer | Posted 02/03/2015
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 23312853
    Species: Human
    Verified Customer | Posted 02/03/2015
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: See PMID 23072705
    Species: Human
    Verified Customer | Posted 02/03/2015
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID 23067388
    Species: Human
    Verified Customer | Posted 02/03/2015
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID 22226689
    Species: Human
    Verified Customer | Posted 02/03/2015
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID 21550429
    Species: Human
    Verified Customer | Posted 02/03/2015
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID 20682625
    Species: Human
    Verified Customer | Posted 02/03/2015

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