Human STRO-1 Antibody

(20 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human STRO-1.
  • Source
    Monoclonal Mouse IgM Clone # STRO-1
  • Purification
    IgM-specific Affinity-purified from hybridoma culture supernatant
  • Immunogen
    Human CD34+ bone marrow cells
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    MG‑63 human osteosarcoma cell line
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
STRO‑1 in Human MG‑63 Cell Line. STRO‑1 was detected in immersion fixed MG‑63 human osteosarcoma cell line using Human STRO‑1 Monoclonal Antibody (Catalog # MAB1038) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgM Secondary Antibody (red; Catalog # NL019) (Catalog # NL019), and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: STRO-1
STRO-1 is a cell surface protein expressed by bone marrow stromal cells and erythroid precursors. The frequency of colony forming units-fibroblasts (CFU-F) was enriched 100-fold in the STRO-1+/Glycophorin A- population from bone marrow cells (1). The subset of marrow cells that expresses the STRO-1 antigen is capable of differentiating into multiple mesenchymal lineages including hematopoiesis-supportive stromal cells with a vascular smooth muscle-like phenotype, adipocytes, osteoblasts, and chondrocytes (2).
  • References:
    1. Simmons, P.J. and B. Torok-Storb (1991) Blood 78:55.
    2. Dennis, J.E. et al. (2002) Cells Tissues Organs 170:73.
  • Long Name:
    A Stromal Cell Precursor Surface Antigen
  • Alternate Names:
    STRO1; STRO-1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Human Dental Pulp Cells Differentiate toward Neuronal Cells and Promote Neuroregeneration in Adult Organotypic Hippocampal Slices In Vitro
    Authors: L Xiao, R Ide, C Saiki, Y Kumazawa, H Okamura
    Int J Mol Sci, 2017;18(8):.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin Emedded
  2. A monoclonal antibody recognizes undifferentiation-specific carbohydrate moieties expressed on cell surface of the human dental pulp cells
    Authors: KJ Kang, SY Ko, CJ Ryu, YJ Jang
    Stem Cell Res, 2017;21(0):85-93.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: Flow
  3. The Mesenchymal Precursor Cell Marker Antibody STRO-1 Binds to Cell Surface Heat Shock Cognate 70
    Stem Cells, 2016;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  4. The differentiation potential of gingival mesenchymal stem cells induced by apical tooth germ cell?conditioned medium
    Mol Med Rep, 2016;14(4):3565-72.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. Restoration of a Critical Mandibular Bone Defect Using Human Alveolar Bone-Derived Stem Cells and Porous Nano-HA/Collagen/PLA Scaffold
    Authors: X Wang, H Xing, G Zhang, X Wu, X Zou, L Feng, D Wang, M Li, J Zhao, J Du, Y Lv, L E, H Liu
    Stem Cells Int, 2016;2016(0):8741641.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC Fresh
  6. Immobilized WNT Proteins Act as a Stem Cell Niche for Tissue Engineering
    Stem Cell Reports, 2016;7(1):126-37.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC - PFA fixed
  7. Human fetal and adult bone marrow-derived mesenchymal stem cells use different signaling pathways for the initiation of chondrogenesis.
    Authors: Brady K, Dickinson S, Guillot P, Polak J, Blom A, Kafienah W, Hollander A
    Stem Cells Dev, 2014;23(5):541-54.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  8. Wnts enhance neurotrophin-induced neuronal differentiation in adult bone-marrow-derived mesenchymal stem cells via canonical and noncanonical signaling pathways.
    Authors: Tsai H, Deng W, Lai W, Chiu W, Yang C, Tsai Y, Hwang S, Renshaw P
    PLoS ONE, 2014;9(8):e104937.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  9. Mesenchymal stem cells engineered to inhibit complement-mediated damage.
    Authors: Soland M, Bego M, Colletti E, Zanjani E, St Jeor S, Porada C, Almeida-Porada G
    PLoS ONE, 2013;8(3):e60461.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: Magnetic Cell Sorting
  10. Intercellular transport of Oct4 in mammalian cells: a basic principle to expand a stem cell niche?
    Authors: Rolf HJ, Niebert S, Niebert M, Gaus L, Schliephake H, Wiese KG
    PLoS ONE, 2012;7(2):e32287.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  11. Characterization of mesenchymal progenitor cells isolated from human bone marrow by negative selection.
    Authors: Modder UI, Roforth MM, Nicks KM, Peterson JM, McCready LK, Monroe DG, Khosla S
    Bone, 2011;50(3):804-10.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  12. High levels of beta-catenin signaling reduce osteogenic differentiation of stem cells in inflammatory microenvironments through inhibition of the noncanonical Wnt pathway.
    Authors: Liu N, Shi S, Deng M, Tang L, Zhang G, Liu N, Ding B, Liu W, Liu Y, Shi H, Liu L, Jin Y
    J. Bone Miner. Res., 2011;26(9):2082-95.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  13. Synovial sarcoma is a stem cell malignancy.
    Authors: Naka N, Takenaka S, Araki N
    Stem Cells, 2010;28(7):1119-31.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  14. Three-dimensional reconstituted extracellular matrix scaffolds for tissue engineering.
    Authors: Narayanan K, Leck KJ, Gao S, Wan AC
    Biomaterials, 2009;30(26):4309-17.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  15. Stem cells from deciduous tooth repair mandibular defect in swine.
    Authors: Zheng Y, Liu Y, Zhang CM
    J. Dent. Res., 2009;88(3):249-54.
    Species: Porcine
    Sample Type: Whole Cells
    Application: ICC
  16. Investigating a clonal human periodontal ligament progenitor/stem cell line in vitro and in vivo.
    Authors: Fujii S, Maeda H, Wada N, Tomokiyo A, Saito M, Akamine A
    J. Cell. Physiol., 2008;215(3):743-9.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  17. Promotion of attachment of human bone marrow stromal cells by CCN2.
    Authors: Ono M, Kubota S, Fujisawa T, Sonoyama W, Kawaki H, Akiyama K, Oshima M, Nishida T, Yoshida Y, Suzuki K, Takigawa M, Kuboki T
    Biochem. Biophys. Res. Commun., 2007;357(1):20-5.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  18. Isolation and characterization of a stem cell population from adult human liver.
    Authors: Herrera MB, Bruno S, Buttiglieri S, Tetta C, Gatti S, Deregibus MC, Bussolati B, Camussi G
    Stem Cells, 2006;24(12):2840-50.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  19. Phenotypic and functional characteristics of mesenchymal stem cells differentiated along a Schwann cell lineage.
    Authors: Caddick J, Kingham PJ, Gardiner NJ, Wiberg M, Terenghi G
    Glia, 2006;54(8):840-9.
    Species: Rat
    Sample Type: Whole Cells
    Application: ICC
  20. Mechanical stress promotes the expression of smooth muscle-like properties in marrow stromal cells.
    Authors: Kobayashi N, Yasu T, Ueba H, Sata M, Hashimoto S, Kuroki M, Saito M, Kawakami M
    Exp. Hematol., 2004;32(12):1238-45.
    Species: Rat
    Sample Type: Whole Cells
    Application: ICC
Expand to show all 20 Citations
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