Human SUMO Pan Specific Antibody

Catalog # Availability Size / Price Qty
A-714
Human SUMO Pan Specific Antibody in Data
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Human SUMO Pan Specific Antibody Summary

Species Reactivity
Human
Specificity
This antibody detects endogenous, human SUMOylated proteins in Western blots, and has similar sensitivity when tested against purified, recombinant SUMO1, SUMO2, SUMO3 and SUMO4.
Source
Monoclonal Rat IgG2A Clone # 852721
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Purified, recombinant human SUMO2
Accession # P61956
Formulation
Supplied as a solution in PBS containing Glycerol and Sodium Azide.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1-0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human SUMO antibody by Western Blot. View Larger

Detection of Human SUMO by Western Blot. Western blot shows lysates of pan-SUMO. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Human SUMO Pan Specific Monoclonal Antibody (Catalog # A-714) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for SUMO at approximately 17-19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -20 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Storage below -20 °C is not recommended. Do not use past expiration date.

Background: SUMO

Small Ubiquitin-like Modifiers (SUMOs) are a family of small, related proteins that can be enzymatically attached to a target protein by a post-translational modification process termed SUMOylation. Unlike ubiquitination which targets proteins for degradation, SUMOylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. All human SUMO proteins share a conserved ubiquitin-like domain and a C-terminal diglycine cleavage/attachment site. Human SUMO1, SUMO2, SUMO3, and SUMO4 are all translated as propeptides, containing C-terminal prosegments following the diglycine motif that marks the end of the mature forms. Following prosegment cleavage, SUMO1, 2, and 3 may then be enzymatically attached to a lysine on a target protein. It is not clear whether SUMO4 is processed in a similar fashion.

Long Name
Small Ubiquitin-like Modifiers
Alternate Names
SUMO

Product Datasheets

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