Human SUMO Pan Specific Antibody
Human SUMO Pan Specific Antibody Summary
Accession # P61956
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human SUMO by Western Blot. Western blot shows lysates of pan-SUMO. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Human SUMO Pan Specific Monoclonal Antibody (Catalog # A-714) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for SUMO at approximately 17-19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Preparation and Storage
Background: SUMO
Small Ubiquitin-like Modifiers (SUMOs) are a family of small, related proteins that can be enzymatically attached to a target protein by a post-translational modification process termed SUMOylation. Unlike ubiquitination which targets proteins for degradation, SUMOylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. All human SUMO proteins share a conserved ubiquitin-like domain and a C-terminal diglycine cleavage/attachment site. Human SUMO1, SUMO2, SUMO3, and SUMO4 are all translated as propeptides, containing C-terminal prosegments following the diglycine motif that marks the end of the mature forms. Following prosegment cleavage, SUMO1, 2, and 3 may then be enzymatically attached to a lysine on a target protein. It is not clear whether SUMO4 is processed in a similar fashion.
Product Datasheets
Product Specific Notices
* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructionsFAQs
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