Human SUMO1 MAb (Clone 852620)

R&D Systems | Catalog # A-716

R&D Systems
Discontinued Product
A-716 has been discontinued. View all SUMO1 products.

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 852620
View all SUMO1 Primary Antibodies »

Product Specifications

Immunogen

Purified, recombinant human SUMO1
Accession # P63165

Specificity

This antibody detects endogenous, human SUMOylated proteins in Western blots. It shows no cross-reactivity against recombinant SUMO2, SUMO3, and SUMO4 in Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human SUMO1 MAb (Clone 852620)

Detection of Human SUMO1 antibody by Western Blot.

Detection of Human SUMO1 by Western Blot.

Western blot shows lysates of SUMO1. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Human SUMO1 Monoclonal Antibody (Catalog # A-716) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for SUMO1 at approximately 17-19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human SUMO1 MAb (Clone 852620)

Application
Recommended Usage

Western Blot

0.1-0.5 µg/mL
Sample: Human SUMO1

Formulation, Preparation, and Storage

Formulation

Supplied as a solution in PBS containing Glycerol and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -20 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Storage below -20 °C is not recommended. Do not use past expiration date.

Background: SUMO1

Small Ubiquitin­like Modifiers (SUMOs) are a family of small, related proteins that can be enzymatically attached to a target protein by a post­translational modification process termed SUMOylation. Unlike ubiquitination, which targets proteins for degradation, SUMOylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. All human SUMO proteins share a conserved ubiquitin-like domain and a C-terminal diglycine cleavage/attachment site. Human SUMO1, also known as Sentrin, UBL1, and SMT3C, is synthesized as a 101 amino acid (aa) propeptide that contains a four aa C­terminal prosegment. Following prosegment cleavage, the C­terminal glycine may be enzymatically attached to a lysine on a target protein. Human SUMO1 shares 100% sequence identity to SUMO1 from mouse. SUMO1 is the most unique of the four identified SUMO proteins and shares only 44%, 47%, and 41% sequence identity to SUMO2, SUMO3, and SUMO4, respectively.

Long Name

Small Ubiquitin-like Modifier 1

Alternate Names

PIC1, Sentrin, SMT3C, SMT3H3, UBL1

Entrez Gene IDs

7341 (Human); 22218 (Mouse); 301442 (Rat)

Gene Symbol

SUMO1

UniProt

P63165

Additional SUMO1 Products

Product Documents for Human SUMO1 MAb (Clone 852620)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human SUMO1 MAb (Clone 852620)

For research use only

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