Human SUMO2/3 Antibody

R&D Systems | Catalog # A-718

Product is in the process of being discontinued.
R&D Systems
Discontinued Product
A-718 has been discontinued. View all SUMO2/3 products.

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 852908
View all SUMO2/3 Primary Antibodies »

Product Specifications

Immunogen

Purified, recombinant human SUMO3
Accession # P55854

Specificity

This antibody detects endogenous, human SUMOylated proteins in Western blots. This antibody has equivalent reactivity to SUMO2 and SUMO3 in Western blots with recombinant SUMO proteins. It has less than 5% cross-reactivity with recombinant SUMO4 andno cross-reactivity with recombinant SUMO1.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human SUMO2/3 Antibody

Detection of Human SUMO2/3 antibody by Western Blot.

Detection of Human SUMO2/3 by Western Blot.

Western blot shows lysates of SUMO2/3. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Human SUMO2/3 Monoclonal Antibody (Catalog # A-718) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for SUMO2/3 at approximately 16-20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human SUMO2/3 antibody by Western Blot.

Detection of Human SUMO2/3 by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human SUMO2/3 Monoclonal Antibody (Catalog # A-718) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for SUMO2/3 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human SUMO2/3 Antibody

Application
Recommended Usage

Western Blot

0.5-1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and MCF‑7 human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

0.5 mg/mL in PBS, pH 7.4, 50% glycerol, and 0.09% sodium azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -20 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Storage below -20 °C is not recommended. Do not use past expiration date.

Background: SUMO2/3

Small Ubiquitin­like Modifiers (SUMOs) are a family of small, related proteins that can be enzymatically attached to a target protein by a post­translational modification process termed SUMOylation. Unlike ubiquitination, which targets proteins for degradation, SUMOylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. All human SUMO proteins share a conserved ubiquitin-like domain and a C-terminal diglycine cleavage/attachment site. Human SUMO1, SUMO2, SUMO3, and SUMO4 are all translated as propeptides, containing C-terminal prosegments following the diglycine motif that marks the end of the mature forms. Following prosegment cleavage, SUMO1, 2, and 3 may then be enzymatically attached to a lysine on a target protein. It is not clear whether SUMO4 is processed in a similar fashion.

Long Name

Small Ubiquitin-like Modifier 2, & 3

Alternate Names

HSMT3, small ubiquitin-like modifier 2, SMT3A, SMT3B, SMT3H2, SUMO2, SUMO3

UniProt

P55854

Additional SUMO2/3 Products

Product Documents for Human SUMO2/3 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human SUMO2/3 Antibody

For research use only

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