Human TACE/ADAM17 Ectodomain Alexa Fluor® 594-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB9301T-100UG
Detection of TACE/ADAM17 in HeLa Human Cell Line by Flow Cytometry
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Human TACE/ADAM17 Ectodomain Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects the ectodomain of human TACE/ADAM17 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with the ectodomain of recombinant human ADAM8, 9, 15 and recombinant mouse ADAM10 is observed.
Source
Monoclonal Mouse IgG1 Clone # 111633
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Insect ovarian cell line T. ni-derived recombinant human TACE/ADAM17
Pro18-Asn671
Accession # P78536
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 594

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of TACE/ADAM17 in HeLa Human Cell Line by Flow Cytometry View Larger

Detection of TACE/ADAM17 in HeLa Human Cell Line by Flow Cytometry HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human TACE/ADAM17 Ectodomain Alexa Fluor® 594‑conjugated Monoclonal Antibody (Catalog # FAB9301T, filled histogram) or isotype control antibody (Catalog # IC002T, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TACE/ADAM17

TACE is a member of the ADAM family that contains A Disintegrin And Metalloprotease-like domain. Like other membrane-anchored ADAMs, TACE consists of a pro domain with a cysteine switch and furin cleavage sequence, a catalytic domain with the zinc-binding site and Met-turn expected for reprolysins, a disintegrin-like domain, a cysteine-rich domain, an EGF-like domain, a transmembrane domain, and the cytoplasmic domain. In addition to its ability to release the 17 kDa extracellular form of Tumor Necrosis Factor-alpha (TNF-alpha ) from the 26 kDa membrane-anchored TNF-alpha, TACE also plays an essential role in shedding ectodomains from a variety of proteins such as L-Selectin, Transforming Growth Factor-alpha, Amyloid Protein Precursor, and Notch-1 receptor. TACE mRNA is present in virtually every tissue and TACE protein resides both on the cell surface and in the cell.

References
  1. Black, R.A. and J.D. Becherer (1998) in Tumor Necrosis Factor alpha -Converting Enzyme. Barrett, A.J. et al. (eds): Handbook of Proteolytic Enzymes, San Diego: Academic Press, p. 1315.
  2. Primakoff, P. and D.G. Myles (2000) Trends in Genetics 16:83.
Long Name
TNF-alpha Converting Enzyme
Entrez Gene IDs
6868 (Human); 11491 (Mouse)
Alternate Names
ADAM 17; ADAM metallopeptidase domain 17; ADAM metallopeptidase domain 18; ADAM17; ADAM18; CD156b antigen; CD156b; CSVP; disintegrin and metalloproteinase domain-containing protein 17; EC 3.4.24.86; MGC71942; Snake venom-like protease; TACE; TACEcSVP; TNF-alpha convertase; TNF-alpha converting enzyme; TNF-alpha-converting enzyme; tumor necrosis factor, alpha, converting enzyme

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