Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 111633
View all TACE/ADAM17 Primary Antibodies »

Product Specifications

Immunogen

Insect ovarian cell line T. ni-derived recombinant human TACE/ADAM17
Pro18-Asn671
Accession # P78536

Specificity

Detects the ectodomain of human TACE/ADAM17 in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with the ectodomain of recombinant human ADAM8, 9, 15 and recombinant mouse ADAM10 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human TACE/ADAM17 Ectodomain PE‑conjugated Antibody

Detection of TACE/ADAM17 in Human PBMC by Flow Cytometry.

Human PBMC were stained with (A) Mouse Anti-Human TACE/ADAM17 Ectodomain PE-conjugated Monoclonal Antibody (Catalog # FAB9301P) or (B) Mouse IgG1 isotype control antibody (IC002P) and Mouse anti-Human CD14 APC-conjugated Monoclonal Antibody (FAB3832A). View our protocol for Staining Membrane-associated Proteins.
Detection of TACE/ADAM17 antibody in HeLa Human Cell Line antibody by Flow Cytometry.

Detection of TACE/ADAM17 in HeLa Human Cell Line by Flow Cytometry.

HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human TACE/ADAM17 Ectodomain PE‑conjugated Monoclonal Antibody (Catalog # FAB9301P, filled histogram) or isotype control antibody (IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human TACE/ADAM17 Ectodomain PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human PBMC and HeLa human cervical epithelial carcinoma cell line

Reviewed Applications

Read 1 review rated 4 using FAB9301P in the following applications:

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TACE/ADAM17

TACE is a member of the ADAM family that contains A Disintegrin And Metalloprotease-like domain. Like other membrane-anchored ADAMs, TACE consists of a pro domain with a cysteine switch and furin cleavage sequence, a catalytic domain with the zinc-binding site and Met-turn expected for reprolysins, a disintegrin-like domain, a cysteine-rich domain, an EGF-like domain, a transmembrane domain, and the cytoplasmic domain. In addition to its ability to release the 17 kDa extracellular form of Tumor Necrosis Factor-alpha (TNF-alpha ) from the 26 kDa membrane-anchored TNF-alpha, TACE also plays an essential role in shedding ectodomains from a variety of proteins such as L-Selectin, Transforming Growth Factor-alpha, Amyloid Protein Precursor, and Notch-1 receptor. TACE mRNA is present in virtually every tissue and TACE protein resides both on the cell surface and in the cell.

References

  1. Black, R.A. and J.D. Becherer (1998) in Tumor Necrosis Factor alpha -Converting Enzyme. Barrett, A.J. et al. (eds): Handbook of Proteolytic Enzymes, San Diego: Academic Press, p. 1315.
  2. Primakoff, P. and D.G. Myles (2000) Trends in Genetics 16:83.

Long Name

TNF-alpha Converting Enzyme

Alternate Names

ADAM17, CD156b

Entrez Gene IDs

6868 (Human); 11491 (Mouse)

Gene Symbol

ADAM17

UniProt

P78536

Additional TACE/ADAM17 Products

Product Documents for Human TACE/ADAM17 Ectodomain PE‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Human TACE/ADAM17 Ectodomain PE‑conjugated Antibody

For research use only

Citations for Human TACE/ADAM17 Ectodomain PE‑conjugated Antibody

Customer Reviews for Human TACE/ADAM17 Ectodomain PE‑conjugated Antibody (1)

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  • Human TACE/ADAM17 Ectodomain PE-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Blood-derived monocytes
    Species: Human
    Verified Customer | Posted 08/01/2017

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