Human TACE/ADAM17 Ectodomain PE-conjugated Antibody

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FAB9301P

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Detection of TACE/ADAM17 in Human PBMC by Flow Cytometry.
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Citations (12)
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Human TACE/ADAM17 Ectodomain PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects the ectodomain of human TACE/ADAM17 in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with the ectodomain of recombinant human ADAM8, 9, 15 and recombinant mouse ADAM10 is observed.
Source
Monoclonal Mouse IgG1 Clone # 111633
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Insect ovarian cell line T. ni-derived recombinant human TACE/ADAM17
Pro18-Asn671
Accession # P78536
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of TACE/ADAM17 in Human PBMC by Flow Cytometry. Human PBMC were stained with (A) Mouse Anti-Human TACE/ADAM17 Ectodomain PE-conjugated Monoclonal Antibody (Catalog # FAB9301P) or (B) Mouse IgG1 isotype control antibody (IC002P) and Mouse anti-Human CD14 APC-conjugated Monoclonal Antibody (FAB3832A). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of TACE/ADAM17 antibody in HeLa Human Cell Line antibody by Flow Cytometry. View Larger

Detection of TACE/ADAM17 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human TACE/ADAM17 Ectodomain PE‑conjugated Monoclonal Antibody (Catalog # FAB9301P, filled histogram) or isotype control antibody (IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TACE/ADAM17

TACE is a member of the ADAM family that contains A Disintegrin And Metalloprotease-like domain. Like other membrane-anchored ADAMs, TACE consists of a pro domain with a cysteine switch and furin cleavage sequence, a catalytic domain with the zinc-binding site and Met-turn expected for reprolysins, a disintegrin-like domain, a cysteine-rich domain, an EGF-like domain, a transmembrane domain, and the cytoplasmic domain. In addition to its ability to release the 17 kDa extracellular form of Tumor Necrosis Factor-alpha (TNF-alpha ) from the 26 kDa membrane-anchored TNF-alpha, TACE also plays an essential role in shedding ectodomains from a variety of proteins such as L-Selectin, Transforming Growth Factor-alpha, Amyloid Protein Precursor, and Notch-1 receptor. TACE mRNA is present in virtually every tissue and TACE protein resides both on the cell surface and in the cell.

References
  1. Black, R.A. and J.D. Becherer (1998) in Tumor Necrosis Factor alpha -Converting Enzyme. Barrett, A.J. et al. (eds): Handbook of Proteolytic Enzymes, San Diego: Academic Press, p. 1315.
  2. Primakoff, P. and D.G. Myles (2000) Trends in Genetics 16:83.
Long Name
TNF-alpha Converting Enzyme
Entrez Gene IDs
6868 (Human); 11491 (Mouse)
Alternate Names
ADAM 17; ADAM metallopeptidase domain 17; ADAM metallopeptidase domain 18; ADAM17; ADAM18; CD156b antigen; CD156b; CSVP; disintegrin and metalloproteinase domain-containing protein 17; EC 3.4.24.86; MGC71942; Snake venom-like protease; TACE; TACEcSVP; TNF-alpha convertase; TNF-alpha converting enzyme; TNF-alpha-converting enzyme; tumor necrosis factor, alpha, converting enzyme

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Citations for Human TACE/ADAM17 Ectodomain PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Chemotherapy‐induced release of ADAM17 bearing EV as a potential resistance mechanism in ovarian cancer
    Authors: Gerrit Hugendieck, Marcus Lettau, Svenja Andreas, Sabrina Neumann, Natalie Reinhardt, Philipp Arnold et al.
    Journal of Extracellular Vesicles
  2. Defective Regulation of Membrane TNF&alpha Expression in Dendritic Cells of Glioblastoma Patients Leads to the Impairment of Cytotoxic Activity against Autologous Tumor Cells
    Authors: T Tyrinova, O Leplina, S Mishinov, M Tikhonova, E Dolgova, A Proskurina, V Stupack, S Bogachev, A Ostanin, E Chernykh
    Int J Mol Sci, 2020-04-21;21(8):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Enhanced STAT3 phosphorylation and PD-L1 expression in myeloid dendritic cells indicate impaired IL-27Ralpha signaling in type 1 diabetes
    Authors: Z Parackova, P Vrabcova, I Zentsova, J Kayserova, I Richtrova, L Sojka, K Stechova, Z Sumnik, A Sediva
    Sci Rep, 2020-01-16;10(1):493.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Metalloproteinases TACE and MMP-9 Differentially Regulate Death Factors on Adult and Neonatal Monocytes After Infection with Escherichia coli
    Authors: S Dreschers, C Platen, A Ludwig, C Gille, N Köstlin, TW Orlikowsky
    Int J Mol Sci, 2019-03-20;20(6):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. An ADAM-10 dependent EPCR shedding links meningococcal interaction with endothelial cells to purpura fulminans
    Authors: H Lécuyer, Z Virion, JP Barnier, S Matczak, S Bourdoulou, E Bianchini, F Saller, D Borgel, X Nassif, M Coureuil
    PLoS Pathog., 2018-04-09;14(4):e1006981.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. ADAM17 inhibition enhances platinum efficiency in ovarian cancer
    Authors: Nina Hedemann, Christoph Rogmans, Susanne Sebens, Daniela Wesch, Manuel Reichert, Dirk Schmidt-Arras et al.
    Oncotarget
  7. NKG2D signaling between human NK cells enhances TACE-mediated TNF-alpha release1
    Authors: Neekun Sharma, Camille V. Trinidad, Andrew P. Trembath, Mary A. Markiewicz
    The Journal of Immunology
  8. NKG2D- and T-cell receptor-dependent lysis of malignant glioma cell lines by human gamma δ T cells: Modulation by temozolomide and A disintegrin and metalloproteases 10 and 17 inhibitors
    Authors: Guranda Chitadze, Marcus Lettau, Stefanie Luecke, Ting Wang, Ottmar Janssen, Daniel Fürst et al.
    OncoImmunology
  9. Unlike for human monocytes after LPS activation, release of TNF-α by THP-1 cells is produced by a TACE catalytically different from constitutive TACE.
    Authors: Moreira-Tabaka H, Peluso J, Vonesch JL, Hentsch D, Kessler P, Reimund JM, Dumont S, Muller CD
    PLoS ONE, 2012-03-30;7(3):e34184.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Increased activation of fibrocytes in patients with chronic obstructive asthma through an epidermal growth factor receptor-dependent pathway.
    Authors: Wang CH, Huang CD, Lin HC, Huang TT, Lee KY, Lo YL, Lin SM, Chung KF, Kuo HP
    J. Allergy Clin. Immunol., 2012-02-09;129(5):1367-76.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Microparticles of human atherosclerotic plaques enhance the shedding of the tumor necrosis factor-alpha converting enzyme/ADAM17 substrates, tumor necrosis factor and tumor necrosis factor receptor-1.
    Authors: Canault M, Leroyer AS, Peiretti F, Leseche G, Tedgui A, Bonardo B, Alessi MC, Boulanger CM, Nalbone G
    Am. J. Pathol., 2007-09-14;171(5):1713-23.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Flow Cytometry
  12. FHL2 interacts with both ADAM-17 and the cytoskeleton and regulates ADAM-17 localization and activity.
    Authors: Canault M, Tellier E, Bonardo B, Mas E, Aumailley M, Juhan-Vague I, Nalbone G, Peiretti F
    J. Cell. Physiol., 2006-08-01;208(2):363-72.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Human TACE/ADAM17 Ectodomain PE-conjugated Antibody
By Anonymous on 08/01/2017
Application: Flow Sample Tested: Blood-derived monocytes Species: Human