Human TACE/ADAM17 Ectodomain Antibody

Catalog # Availability Size / Price Qty
MAB9301-SP
MAB9301-500
MAB9301-100
Human TACE/ADAM17 Ectodomain Antibody in Flow Cytometry
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Product Details
Citations (10)
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Human TACE/ADAM17 Ectodomain Antibody Summary

Species Reactivity
Human
Specificity
Detects the ectodomain of human TACE/ADAM17 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with the ectodomain of recombinant human ADAM8, 9, 15 and recombinant mouse ADAM10 is observed.
Source
Monoclonal Mouse IgG1 Clone # 111633
Purification
Protein A or G purified from ascites
Immunogen
Insect ovarian cell line T. ni-derived recombinant human TACE/ADAM17
Pro18-Asn671
Accession # P78536
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human TACE/ADAM17 Western Blot Standard (Catalog # WBC029) under non-reducing conditions only
Flow Cytometry
0.25 µg/106 cells
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human TACE/ADAM17 (Catalog # 930-ADB), see our available Western blot detection antibodies
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Knockout Validated
TACE/ADAM17 is specifically detected in HeLa human carcinoma parental cell line but is not detectable in TACE/ADAM17 knockout HeLa cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of TACE/ADAM17 in HeLa Human Cell Line by Flow Cytometry. View Larger

Detection of TACE/ADAM17 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human TACE/ADAM17 Ectodomain Monoclonal Antibody (Catalog # MAB9301, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Knockout Validated TACE/ADAM17 Specificity is Shown by Flow Cytometry in Knockout Cell Line. View Larger

TACE/ADAM17 Specificity is Shown by Flow Cytometry in Knockout Cell Line. TACE/ADAM17 knockout HeLa epithelial carcinoma cell line was stained with Mouse Anti-Human TACE/ADAM17 Monoclonal Antibody (Catalog # MAB9301, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by PE-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0102B). No staining in the TACE/ADAM17 knockout HeLa cell line was observed. View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TACE/ADAM17

TACE is a member of the ADAM family that contains A Disintegrin And Metalloprotease-like domain. Like other membrane-anchored ADAMs, TACE consists of a pro domain with a cysteine switch and furin cleavage sequence, a catalytic domain with the zinc-binding site and Met-turn expected for reprolysins, a disintegrin-like domain, a cysteine-rich domain, an EGF-like domain, a transmembrane domain, and the cytoplasmic domain. In addition to its ability to release the 17 kDa extracellular form of Tumor Necrosis Factor-alpha (TNF-alpha ) from the 26 kDa membrane-anchored TNF-alpha, TACE also plays an essential role in shedding ectodomains from a variety of proteins such as L-Selectin, Transforming Growth Factor-alpha, Amyloid Protein Precursor, and Notch-1 receptor. TACE mRNA is present in virtually every tissue and TACE protein resides both on the cell surface and in the cell.

References
  1. Black, R.A. and J.D. Becherer (1998) in Tumor Necrosis Factor alpha -Converting Enzyme. Barrett, A.J. et al. (eds): Handbook of Proteolytic Enzymes, San Diego: Academic Press, p. 1315.
  2. Primakoff, P. and D.G. Myles (2000) Trends in Genetics 16:83.
Long Name
TNF-alpha Converting Enzyme
Entrez Gene IDs
6868 (Human); 11491 (Mouse)
Alternate Names
ADAM 17; ADAM metallopeptidase domain 17; ADAM metallopeptidase domain 18; ADAM17; ADAM18; CD156b antigen; CD156b; CSVP; disintegrin and metalloproteinase domain-containing protein 17; EC 3.4.24.86; MGC71942; Snake venom-like protease; TACE; TACEcSVP; TNF-alpha convertase; TNF-alpha converting enzyme; TNF-alpha-converting enzyme; tumor necrosis factor, alpha, converting enzyme

Product Datasheets

Citations for Human TACE/ADAM17 Ectodomain Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Ectodomain Shedding of the Cell Adhesion Molecule Nectin-4 in Ovarian Cancer is Mediated by ADAM10 and ADAM17
    Authors: PC Buchanan, KL Boylan, B Walcheck, R Heinze, MA Geller, PA Argenta, AP Skubitz
    J. Biol. Chem, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Increasing TIMP3 expression by hypomethylating agents diminishes soluble MICA, MICB and ULBP2 shedding in acute myeloid leukemia, facilitating NK cell-mediated immune recognition
    Authors: AB Raneros, AM Puras, RM Rodriguez, E Colado, T Bernal, E Anguita, AV Mogorron, AC Gil, JR Vidal-Cast, L Márquez-Ki, PD Bulnes, AM Marin, MCG Garay, B Suarez-Alv, C Lopez-Larr
    Oncotarget, 2017;8(19):31959-31976.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Genotoxic Stress Induces Senescence-Associated ADAM10-Dependent Release of NKG2D MIC Ligands in Multiple Myeloma Cells.
    Authors: Zingoni A, Cecere F, Vulpis E, Fionda C, Molfetta R, Soriani A, Petrucci M, Ricciardi M, Fuerst D, Amendola M, Mytilineos J, Cerboni C, Paolini R, Cippitelli M, Santoni A
    J Immunol, 2015;195(2):736-48.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Identification of an ADAM17 cleavage region in human CD16 (FcgammaRIII) and the engineering of a non-cleavable version of the receptor in NK cells.
    Authors: Jing Y, Ni Z, Wu J, Higgins L, Markowski T, Kaufman D, Walcheck B
    PLoS ONE, 2015;10(3):e0121788.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Expansion of necrotic core and shedding of Mertk receptor in human carotid plaques: a role for oxidized polyunsaturated fatty acids?
    Cardiovasc Res, 2013;97(1):125-33.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. A disintegrin and metalloproteinase 17 (ADAM17) and epidermal growth factor receptor (EGFR) signaling drive the epithelial response to Staphylococcus aureus toxic shock syndrome toxin-1 (TSST-1).
    Authors: Breshears L, Schlievert P, Peterson M
    J Biol Chem, 2012;287(39):32578-87.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. The sheddase activity of ADAM17/TACE is regulated by the tetraspanin CD9.
    Authors: Gutierrez-Lopez MD, Gilsanz A, Yáñez-Mó M, Ovalle S, Lafuente EM, Dominguez C, Monk PN, Gonzalez-Alvaro I, Sanchez-Madrid F, Cabanas C
    Cell. Mol. Life Sci., 2011;68(19):3275-92.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  8. Tissue inhibitors of matrix metalloproteinases in platelets and megakaryocytes: a novel organization for these secreted proteins.
    Authors: Villeneuve J, Block A, Le Bousse-Kerdiles MC, Lepreux S, Nurden P, Ripoche J, Nurden AT
    Exp. Hematol., 2009;37(7):849-56.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  9. Regulated release and functional modulation of junctional adhesion molecule A by disintegrin metalloproteinases.
    Authors: Koenen RR, Pruessmeyer J, Soehnlein O, Fraemohs L, Zernecke A, Schwarz N, Reiss K, Sarabi A, Lindbom L, Hackeng TM, Weber C, Ludwig A
    Blood, 2009;113(19):4799-809.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Expression of ADAM-17, TIMP-3 and fractalkine in the human adult brain endothelial cell line, hCMEC/D3, following pro-inflammatory cytokine treatment.
    Authors: Hurst LA, Bunning RA, Couraud PO, Romero IA, Weksler BB, Sharrack B, Woodroofe MN
    J. Neuroimmunol., 2009;210(1):108-12.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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