Human TACE/ADAM17 Ectodomain Antibody

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Detection of TACE/ADAM17 in HeLa Human Cell Line by Flow Cytometry.
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Citations (17)
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Human TACE/ADAM17 Ectodomain Antibody Summary

Species Reactivity
Detects the ectodomain of human TACE/ADAM17 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with the ectodomain of recombinant human ADAM8, 9, 15 and recombinant mouse ADAM10 is observed.
Monoclonal Mouse IgG1 Clone # 111633
Protein A or G purified from hybridoma culture supernatant
Insect ovarian cell line T. ni-derived recombinant human TACE/ADAM17
Accession # P78536
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
Recombinant Human TACE/ADAM17 Western Blot Standard (Catalog # WBC029) under non-reducing conditions only
Flow Cytometry
0.25 µg/106 cells
See below
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human TACE/ADAM17 (Catalog # 930-ADB), see our available Western blot detection antibodies
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Knockout Validated
TACE/ADAM17 is specifically detected in HeLa human carcinoma parental cell line but is not detectable in TACE/ADAM17 knockout HeLa cell line.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of TACE/ADAM17 antibody in HeLa Human Cell Line antibody by Flow Cytometry. View Larger

Detection of TACE/ADAM17 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human TACE/ADAM17 Ectodomain Monoclonal Antibody (Catalog # MAB9301, filled histogram) or isotype control antibody (MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). View our protocol for Staining Membrane-associated Proteins.

Knockout Validated TACE/ADAM17 Antibody Specificity is Shown by Flow Cytometry antibody in Knockout Cell Line. View Larger

TACE/ADAM17 Specificity is Shown by Flow Cytometry in Knockout Cell Line. TACE/ADAM17 knockout HeLa epithelial carcinoma cell line was stained with Mouse Anti-Human TACE/ADAM17 Monoclonal Antibody (Catalog # MAB9301, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by PE-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0102B). No staining in the TACE/ADAM17 knockout HeLa cell line was observed. View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of Human TACE/ADAM17 by Flow Cytometry View Larger

Detection of Human TACE/ADAM17 by Flow Cytometry Effects of the engineered S197P mutation on CD16a shedding in NK cells.NK92 cells transduced with empty vector (vector only), CD16a, or CD16a/S197P were treated without (Unstim.) or with PMA (100ng/ml) for 30 minutes at 37°C (A), with IL-12 and IL-18 (100ng/ml and 400ng/ml, respectively) for 24 hours at 37°C (B), or with Raji cells and rituximab for 60 min at 37°C (C). Cell surface levels of CD16a were determined by flow cytometry. Isotype-matched negative control antibody staining is indicated by a dotted line. (D) Parent NK92 cells and transduced cells expressing CD16a or CD16a/S197P were treated with Raji cells and rituximab in the presence or absence of the ADAM17 inhibitor BMS566394 (5μM) for 60 min at 37°C. Soluble CD16a levels were determined by ELISA. Each treatment condition was repeated 3 times and the data are representative of 3 independent experiments. Bar graphs show mean ± SD. Statistical significance is indicated as ***P<0.001. (E) NK92 cells expressing CD16a or CD16a/S197P were stained with the anti-ADAM17 mAbs M220, 623, 633, or an isotype-matched negative control antibody, as indicated. (F) CD56+CD45+ NK cells derived from mock-transduced iPSCs (left panel) or iPSCs expressing recombinant CD16a or CD16a/S197P (right panels) were incubated with or without K562 target cells for 4 hours at 37°C. For all histogram plots, the x-axis = Log 10 fluorescence, the y-axis = cell number, and the data are representative of at least 3 independent experiments. Image collected and cropped by CiteAb from the following publication (, licensed under a CC-BY license. Not internally tested by R&D Systems.

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TACE/ADAM17

TACE is a member of the ADAM family that contains A Disintegrin And Metalloprotease-like domain. Like other membrane-anchored ADAMs, TACE consists of a pro domain with a cysteine switch and furin cleavage sequence, a catalytic domain with the zinc-binding site and Met-turn expected for reprolysins, a disintegrin-like domain, a cysteine-rich domain, an EGF-like domain, a transmembrane domain, and the cytoplasmic domain. In addition to its ability to release the 17 kDa extracellular form of Tumor Necrosis Factor-alpha (TNF-alpha ) from the 26 kDa membrane-anchored TNF-alpha, TACE also plays an essential role in shedding ectodomains from a variety of proteins such as L-Selectin, Transforming Growth Factor-alpha, Amyloid Protein Precursor, and Notch-1 receptor. TACE mRNA is present in virtually every tissue and TACE protein resides both on the cell surface and in the cell.

  1. Black, R.A. and J.D. Becherer (1998) in Tumor Necrosis Factor alpha -Converting Enzyme. Barrett, A.J. et al. (eds): Handbook of Proteolytic Enzymes, San Diego: Academic Press, p. 1315.
  2. Primakoff, P. and D.G. Myles (2000) Trends in Genetics 16:83.
Long Name
TNF-alpha Converting Enzyme
Entrez Gene IDs
6868 (Human); 11491 (Mouse)
Alternate Names
ADAM 17; ADAM metallopeptidase domain 17; ADAM metallopeptidase domain 18; ADAM17; ADAM18; CD156b antigen; CD156b; CSVP; disintegrin and metalloproteinase domain-containing protein 17; EC; MGC71942; Snake venom-like protease; TACE; TACEcSVP; TNF-alpha convertase; TNF-alpha converting enzyme; TNF-alpha-converting enzyme; tumor necrosis factor, alpha, converting enzyme

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Citations for Human TACE/ADAM17 Ectodomain Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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  1. ADAM17 targeting by human cytomegalovirus remodels the cell surface proteome to simultaneously regulate multiple immune pathways
    Authors: Rubina, A;Patel, M;Nightingale, K;Potts, M;Fielding, CA;Kollnberger, S;Lau, B;Ladell, K;Miners, KL;Nichols, J;Nobre, L;Roberts, D;Trinca, TM;Twohig, JP;Vlahava, VM;Davison, AJ;Price, DA;Tomasec, P;Wilkinson, GWG;Weekes, MP;Stanton, RJ;Wang, ECY;
    Proceedings of the National Academy of Sciences of the United States of America
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Deletion of the non-adjacent genes UL148 and UL148D impairs human cytomegalovirus-mediated TNF receptor 2 surface upregulation
    Authors: Vu Thuy Khanh Le-Trilling, Fabienne Maa beta en, Benjamin Katschinski, Hartmut Hengel, Mirko Trilling
    Frontiers in Immunology
  3. Adherens junctions organize size-selective proteolytic hotspots critical for Notch signalling
    Authors: M Kwak, KM Southard, WR Kim, A Lin, NH Kim, R Gopalappa, HJ Lee, M An, SH Choi, Y Jung, K Noh, J Farlow, A Georgakopo, NK Robakis, MK Kang, ML Kutys, D Seo, HH Kim, YH Kim, J Cheon, ZJ Gartner, YW Jun
    Nature Cell Biology, 2022-12-01;24(12):1739-1753.
    Species: Human
    Sample Types: Transfected Whole Cells
    Applications: ICC
  4. Systemic Immune Dysfunction in Cancer Patients Driven by IL6 Induction of LAG3 in Peripheral CD8+ T Cells.
    Authors: Somasundaram A, Cillo A, Lampenfeld C, Workman C, Kunning S, Oliveri L, Velez M, Joyce S, Calderon M, Dadey R, Rajasundaram D, Normolle D, Watkins S, Herman J, Kirkwood J, Lipson E, Ferris R, Bruno T, Vignali D
    Cancer Immunol Res, 2022-07-01;10(7):885-899.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Inflammatory activation of surface molecule shedding by upregulation of the pseudoprotease iRhom2 in colon epithelial cells
    Authors: AA Giese, A Babendreye, P Krappen, A Gross, P Strnad, S Düsterhöft, A Ludwig
    Scientific Reports, 2021-12-20;11(1):24230.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Flow Cytometry, Western Blot
  6. A Genetically Engineered Primary Human Natural Killer Cell Platform for Cancer Immunotherapy
    Authors: EJ Pomeroy, JT Hunzeker, MG Kluesner, WS Lahr, BA Smeester, MR Crosby, CL Lonetree, K Yamamoto, L Bendzick, JS Miller, MA Geller, B Walcheck, M Felices, BR Webber, TK Starr, BS Moriarity
    Mol. Ther., 2019-10-15;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Increasing TIMP3 expression by hypomethylating agents diminishes soluble MICA, MICB and ULBP2 shedding in acute myeloid leukemia, facilitating NK cell-mediated immune recognition
    Authors: AB Raneros, AM Puras, RM Rodriguez, E Colado, T Bernal, E Anguita, AV Mogorron, AC Gil, JR Vidal-Cast, L Márquez-Ki, PD Bulnes, AM Marin, MCG Garay, B Suarez-Alv, C Lopez-Larr
    Oncotarget, 2017-05-09;8(19):31959-31976.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Ectodomain Shedding of the Cell Adhesion Molecule Nectin-4 in Ovarian Cancer is Mediated by ADAM10 and ADAM17
    Authors: PC Buchanan, KL Boylan, B Walcheck, R Heinze, MA Geller, PA Argenta, AP Skubitz
    J. Biol. Chem, 2017-02-23;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. The sorting protein PACS-2 promotes ErbB signalling by regulating recycling of the metalloproteinase ADAM17
    Authors: Sarah Louise Dombernowsky, Jacob Samsøe-Petersen, Camilla Hansson Petersen, Rachael Instrell, Anne-Mette Bornhardt Hedegaard, Laurel Thomas et al.
    Nature Communications
  10. Genotoxic Stress Induces Senescence-Associated ADAM10-Dependent Release of NKG2D MIC Ligands in Multiple Myeloma Cells.
    Authors: Zingoni A, Cecere F, Vulpis E, Fionda C, Molfetta R, Soriani A, Petrucci M, Ricciardi M, Fuerst D, Amendola M, Mytilineos J, Cerboni C, Paolini R, Cippitelli M, Santoni A
    J Immunol, 2015-06-12;195(2):736-48.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Identification of an ADAM17 cleavage region in human CD16 (FcgammaRIII) and the engineering of a non-cleavable version of the receptor in NK cells.
    Authors: Jing Y, Ni Z, Wu J, Higgins L, Markowski T, Kaufman D, Walcheck B
    PLoS ONE, 2015-03-27;10(3):e0121788.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Expansion of necrotic core and shedding of Mertk receptor in human carotid plaques: a role for oxidized polyunsaturated fatty acids?
    Cardiovasc Res, 2012-09-20;97(1):125-33.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. A disintegrin and metalloproteinase 17 (ADAM17) and epidermal growth factor receptor (EGFR) signaling drive the epithelial response to Staphylococcus aureus toxic shock syndrome toxin-1 (TSST-1).
    Authors: Breshears L, Schlievert P, Peterson M
    J Biol Chem, 2012-07-25;287(39):32578-87.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. The sheddase activity of ADAM17/TACE is regulated by the tetraspanin CD9.
    Authors: Gutierrez-Lopez MD, Gilsanz A, Yáñez-Mó M, Ovalle S, Lafuente EM, Dominguez C, Monk PN, Gonzalez-Alvaro I, Sanchez-Madrid F, Cabanas C
    Cell. Mol. Life Sci., 2011-03-02;68(19):3275-92.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  15. Tissue inhibitors of matrix metalloproteinases in platelets and megakaryocytes: a novel organization for these secreted proteins.
    Authors: Villeneuve J, Block A, Le Bousse-Kerdiles MC, Lepreux S, Nurden P, Ripoche J, Nurden AT
    Exp. Hematol., 2009-05-03;37(7):849-56.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  16. Expression of ADAM-17, TIMP-3 and fractalkine in the human adult brain endothelial cell line, hCMEC/D3, following pro-inflammatory cytokine treatment.
    Authors: Hurst LA, Bunning RA, Couraud PO, Romero IA, Weksler BB, Sharrack B, Woodroofe MN
    J. Neuroimmunol., 2009-03-25;210(1):108-12.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  17. Regulated release and functional modulation of junctional adhesion molecule A by disintegrin metalloproteinases.
    Authors: Koenen RR, Pruessmeyer J, Soehnlein O, Fraemohs L, Zernecke A, Schwarz N, Reiss K, Sarabi A, Lindbom L, Hackeng TM, Weber C, Ludwig A
    Blood, 2009-03-03;113(19):4799-809.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry


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Human TACE/ADAM17 Ectodomain Antibody
By Anonymous on 09/30/2021
Application: WB Sample Tested: A431 cells Species: Human