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Human TFF2 Alexa Fluor® 647-conjugated Antibody

Catalog #: FAB4077R Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB4077R-100UG

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Human TFF2 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human TFF2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human TFF3.
Source
Monoclonal Mouse IgG2b Clone # 366508
Purification
Protein A or G purified
Immunogen
E. coli-derived recombinant human TFF2
Glu24-Tyr129
Accession # Q03403
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: TFF2

Trefoil Factor 2 (TFF2), previously called spasmolysin, is one of three trefoil peptides secreted by epithelial cells that line mucus membranes. Trefoils contribute to protection and repair of the gastrointestinal tract. TFF2 is secreted by epithelia of the basal gastric glands and duodenal Brunner’s glands, but is also found in spleen, thymus and lung and may modulate leukocyte recruitment to sites of inflammation in these tissues. The 129 amino acid (aa) human TFF2 contains two trefoil structures formed by intramolecular disulfides and shows 82% aa identity with mouse TFF2.

Long Name
Trefoil Factor 2
Entrez Gene IDs
7032 (Human); 21785 (Mouse); 116592 (Rat)
Alternate Names
SML1; SML1trefoil factor 2, SML1, human spasmolytic polypeptide (SP)10spasmolytic protein 1; SP; Spasmolysin; Spasmolytic Polypeptide; TFF2; trefoil factor 2

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