Human TFF2 Antibody

Catalog # Availability Size / Price Qty
TFF2 in Human Stomach.
1 Image
Product Details
Citations (1)
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Human TFF2 Antibody Summary

Species Reactivity
Detects human TFF2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human TFF3.
Monoclonal Mouse IgG2B Clone # 366508
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human TFF2
Accession # Q03403
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Recommended Concentration
Western Blot
1 µg/mL
Recombinant Human TFF2
1-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry TFF2 antibody in Human Stomach by Immunohistochemistry (IHC-P). View Larger

TFF2 in Human Stomach. TFF2 was detected in immersion fixed paraffin-embedded sections of human stomach using Mouse Anti-Human TFF2 Monoclonal Antibody (Catalog # MAB4077) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to gastric glands. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TFF2

Trefoil Factor 2 (TFF2), previously called spasmolysin, is one of three trefoil peptides secreted by epithelial cells that line mucus membranes. Trefoils contribute to protection and repair of the gastrointestinal tract. TFF2 is secreted by epithelia of the basal gastric glands and duodenal Brunner’s glands, but is also found in spleen, thymus and lung and may modulate leukocyte recruitment to sites of inflammation in these tissues. The 129 amino acid (aa) human TFF2 contains two trefoil structures formed by intramolecular disulfides and shows 82% aa identity with mouse TFF2.

Long Name
Trefoil Factor 2
Entrez Gene IDs
7032 (Human); 21785 (Mouse); 116592 (Rat)
Alternate Names
SML1; SML1trefoil factor 2, SML1, human spasmolytic polypeptide (SP)10spasmolytic protein 1; SP; Spasmolysin; Spasmolytic Polypeptide; TFF2; trefoil factor 2

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Citation for Human TFF2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Nardilysin regulates inflammation, metaplasia, and tumors in murine stomach
    Authors: Y Kimura, K Ikuta, T Kimura, T Chiba, H Oshima, M Oshima, E Nishi, H Seno
    Sci Rep, 2017;7(0):43052.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC


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