Human TFPI Quantikine ELISA Kit

  (7 citations)     
Datasheet / CoA / SDS
Product Details
Assay Procedure
Citations (7)
FAQs
Supplemental Products
Reviews
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    3.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Citrate Plasma (10 uL), Urine (50 uL)
  • Sensitivity
    6.7 pg/mL
  • Assay Range
    31.2 - 2,000 pg/mL (Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine)
  • Specificity
    Natural and recombinant human TFPI. This kit recognizes predominantly free TFPI and a very small percentage of the LDL- and HDL-bound TFPI.
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    Interference observed with 1 or more available related molecules.
Control Available
QC167 , Quantikine Immunoassay Control Set 860 for Human TFPI - Please Inquire
Product Summary
The Quantikine Human TFPI Immunoassay is a 3.5 hour solid-phase ELISA designed to measure human TFPI in cell culture supernates, plasma, and urine. It contains NS0-expressed recombinant human TFPI and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human TFPI showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human TFPI.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 299 554 1108 301 569 1127
Standard Deviation 13.5 22.2 40.3 18.5 30.6 66.2
CV% 4.5 4 3.6 6.1 5.4 5.9

Recovery

The recovery of TFPI spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 92 85-100
Urine (n=4) 94 85-106
Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of TFPI were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
 TFPI [HRP]
Product Datasheets

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: TFPI
Human TFPI is a physiological inhibitor of the extrinsic pathway of coagulation and functions in anticoagulation and anti-inflammation. It is a secreted protein with an N-terminal acidic region, three Kunitz (K) domains separated by two linker regions, and a C-terminal basic region. The first K domain inhibits coagulation factor VIIa complexed to tissue factor (TF). The second K domain inhibits factor Xa. The third K domain binds to heparin. The C-terminal basic region may have several functions. Human TFPI-2 is structurally similar to TFPI and is downregulated in several cancers.
  • Long Name:
    Tissue Factor Pathway Inhibitor
  • Entrez Gene IDs:
    7035 (Human); 21788 (Mouse)
  • Alternate Names:
    convertin; EPI; EPITFPI1LACITFI; Extrinsic pathway inhibitor; LACI; Lipoprotein-associated coagulation inhibitor; TFPI; tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor); tissue factor pathway inhibitor
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour on the shaker.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
Filter your results:

Species
Sample Type
  1. Increased circulating procoagulant and anticoagulant factors as TF and TFPI according to severity or infecting serotypes in human dengue infection
    Authors: E Leal de Az, VE Solórzano, DB de Oliveir, CF Marinho, LJ de Souza, RV da Cunha, PV Damasco, CF Kubelka, LM de-Oliveir
    Microbes Infect, 2017;19(1):62-68.
    Species: Human
    Sample Type: Plasma
  2. Dual inhibition of thrombin and activated factor X attenuates disseminated intravascular coagulation and protects organ function in a baboon model of severe Gram-negative sepsis
    Authors: H Schöchl, M van Griens, S Heitmeier, V Laux, U Kipman, J Roodt, S Bahrami, H Redl
    Crit Care, 2017;21(1):51.
    Species: Primate - Papio ursinus (Chacma baboon)
    Sample Type: Plasma
  3. MiR-205 and MiR-373 Are Associated with Aggressive Human Mucinous Colorectal Cancer
    Authors: Annette Eyking
    PLoS ONE, 2016;11(6):e0156871.
    Species: Human
    Sample Type: Cell Culture Supernates
  4. Tissue factor is induced by interleukin-33 in human endothelial cells: a new link between coagulation and inflammation
    Sci Rep, 2016;6(0):25171.
    Species: Human
    Sample Type: Cell Culture Supernates
  5. The C-terminus of tissue factor pathway inhibitor alpha is required for its interaction with factors V and Va.
    J. Thromb. Haemost., 2012;10(9):1944-6.
    Species: Human
    Sample Type: Plasma
  6. TFPIbeta is the GPI-anchored TFPI isoform on human endothelial cells and placental microsomes.
    Authors: Girard TJ, Tuley E, Broze GJ
    Blood, 2012;119(5):1256-62.
    Species: Human
    Sample Type: Cell Culture Supernates
  7. Venous thrombosis associated with gene deletion of tissue factor pathway inhibitor.
    Authors: Kentsis A, Bradwin G, Miller DT, Trenor CC
    Am. J. Hematol., 2009;84(11):775-6.
    Species: Human
    Sample Type: Plasma

FAQs

No product specific FAQs exist for this product, however you may

View all ELISA FAQs
ELISA Controls
Description Application Cat# Citations Images  

Quantikine Immunoassay Control Set 860 for Human TFPI

Ctrl QC167
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Supplemental ELISA Products
Description Application Cat# Citations Images  

Quantikine ELISA Wash Buffer 1

ELISA WA126 6  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Human TFPI Quantikine ELISA Kit?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Us to Order
reload

Feedback

Customer Information
R&D Systems Guarantee