This antibody has been validated by flow cytometry on TGF-beta RII-transfected cells only. In contrast to Catalog # FAB2411A
, minimal staining of PBMC lymphocytes was observed with this antibody.
Monoclonal Mouse IgG1 Clone # 25508
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human TGF-beta RII
Accession # P37173.2
Supplied in a saline solution containing BSA and Sodium Azide.
Alexa Fluor 700
5 µL/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols
are available in the Technical Information section on our website.
Detection of TGF‑ beta RII in NS0 Mouse Cell Line Transfected with Human TGF‑ beta RII by Flow Cytometry. NS0 mouse myeloma cell line transfected with human TGF‑ beta RII was stained with Mouse Anti-Human TGF‑ beta RII Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB241N, filled histogram) or isotype control antibody (Catalog # IC002N, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
Background: TGF-beta RII
TGF-beta RII is a membrane bound serine/threonine kinase. Upon ligand binding, TGF-beta RII interacts with TGF-beta RI to form the heteromeric signaling complex that transduces TGF-beta signals. A splice variant of the type II receptor, TGF-beta RIIb, containing a 25 amino acid residue insertion near the N-terminus of the mature protein has also been described.
Transforming Growth Factor beta Receptor II
Entrez Gene IDs:
7048 (Human); 21813 (Mouse)
TGFBR2; AAT3; EC 2.7.11; EC 126.96.36.199; FAA3; HNPCC6; LDS1B; LDS2B; MFS2; RIIC; TAAD2; tbetaR-II; TbetaR-II; TGF-beta receptor type II; TGF-beta receptor type IIB; TGF-beta receptor type-2; TGF-beta type II receptor; TGFbeta-RII; TGFR-2; transforming growth factor beta receptor type IIC; transforming growth factor, beta receptor II (70/80kDa); transforming growth factor, beta receptor II (70/80kDa) isoform 1; transforming growth factor, beta receptor II (70/80kDa) isoform 2; transforming growth factor, beta receptor II (70-80kD); Transforming growth factor-beta receptor type II
Related Research Areas