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Human TGF-beta  RII PerCP-conjugated Antibody

    
Datasheet / CoA / SDS
Detection of TGF-beta  RII in NS0 Mouse Cell Line Transfected with TGF-beta  RII by Flow Cytometry.

Flow Cytometry

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  • Species Reactivity
    Human
  • Specificity
    This antibody has been validated by flow cytometry on TGF-beta RII-transfected cells only. In contrast to Catalog # FAB2411A, minimal staining of PBMC lymphocytes was observed with this antibody.
  • Source
    Monoclonal Mouse IgG1 Clone # 25508
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human TGF-beta RII
    Ile24-Asp159
    Accession # P37173.2
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    PerCP (Peridinin-chlorophyll Protein Complex)
Product Datasheets
Product Datasheet

COA

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SDS
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Flow Cytometry
Flow Cytometry TGF-beta RII Antibody (25508) [PerCP (Peridinin-chlorophyll Protein Complex)]
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Detection of TGF-beta  RII in NS0 Mouse Cell Line Transfected with TGF-beta  RII by Flow Cytometry. NS0 mouse myeloma cell line transfected with TGF-beta  RII was stained with Mouse Anti-Human TGF-beta  RII PerCP-conjugated Monoclonal Antibody (Catalog # FAB241C, filled histogram) or isotype control antibody (Catalog # IC002C, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TGF-beta RII

TGF-beta RII is a membrane bound serine/threonine kinase. Upon ligand binding, TGF-beta RII interacts with TGF-beta RI to form the heteromeric signaling complex that transduces TGF-beta signals. A splice variant of the type II receptor, TGF-beta RIIb, containing a 25 amino acid residue insertion near the N-terminus of the mature protein has also been described.

  • Long Name:
    Transforming Growth Factor beta Receptor II
  • Entrez Gene IDs:
    7048 (Human); 21813 (Mouse)
  • Alternate Names:
    AAT3; EC 2.7.11; EC 2.7.11.30; FAA3; HNPCC6; LDS1B; LDS2B; MFS2; RIIC; TAAD2; tbetaR-II; TGF-beta receptor type II; TGF-beta receptor type IIB; TGF-beta receptor type-2; TGF-beta RII; TGF-beta type II receptor; TGFbetaRII; TGFbeta-RII; TGFBR2; TGF-bRII; TGFR-2; transforming growth factor beta receptor type IIC; transforming growth factor, beta receptor II (70/80kDa) isoform 1; transforming growth factor, beta receptor II (70/80kDa) isoform 2; transforming growth factor, beta receptor II (70/80kDa); transforming growth factor, beta receptor II (70-80kD); Transforming growth factor-beta receptor type II
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FAQs

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Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 PerCP-conjugated Antibody

 Ctrl IC002C  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

 Flow FC001 7
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Flow Cytometry Mouse Lyse Buffer (10X)

 Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

 Flow FC002 1
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