Human TGR5/GPBAR1 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB4286P
Detection  of TGR5/GPBAR1 in HEK293 Human Cell Line by Flow Cytometry.
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Human TGR5/GPBAR1 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Stains human TGR5/GPBAR1 transfectants but not irrelevant transfectants in flow cytometry.
Source
Monoclonal Mouse IgG2B Clone # 409522
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human TGR5/GPBAR1
Met1-Asn330
Accession # Q8TDU6
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection  of TGR5/GPBAR1 in HEK293 Human Cell Line by Flow Cytometry. View Larger

Detection of TGR5/GPBAR1 in HEK293 Human Cell Line by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human TGR5/GPBAR1 and eGFP was stained with either (A) Mouse Anti-Human TGR5/GPBAR1 PE‑conjugated Monoclonal Antibody (Catalog # FAB4286P) or (B) Mouse IgG2B Phycoerythrin Isotype Control (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TGR5/GPBAR1

TGR5, also known as GPR131, GPBAR1, M-BAR, and BG37, is a 46-48 kDa member of the GPCR1 family of molecules. It is a 330 amino acid (aa) 7-transmembrane glycoprotein that is found on a wide variety of cell types. These include monocytes, macrophages, Kupffer cells, skeletal muscle and pancreatic acinar cells, autonomic enteric and sensory DRG neurons, liver sinusoidal endothelial cells, intestinal L and EC (enterochromaffin) cells, gallbladder epithelium and cholangiocytes (bile duct epithelium). Notably, TGR5 expression exhibits polarity with respect to plasma membrane location, and it may appear either on the cell surface or in intracellualr membrane compartments such as the ER, nucleus or multivesicular bodies. Initially considered to be coupled to G alpha s with cAMP production, it is now known to also interact with G alpha i, activating the ERK signaling pathway. Bile acids generated from cholesterol in hepatocytes are a known ligand for TGR5. Aside from their role in fatty acid absorption, BA ligation of macrophage/Kupffer cell TGR5 results in an anti-inflammatory environment. Over the aa that constitute the TGR5 extracellular domain, human and mouse share 73% aa sequence identity; over the entire ORF, human and mouse share 83% aa sequence identity.

Long Name
Bile Acid Receptor TGR5
Entrez Gene IDs
151306 (Human); 227289 (Mouse); 338443 (Rat)
Alternate Names
BG37; BG37hBG37; G protein-coupled bile acid receptor 1; GPBA; GPBAR1; GPCR19; GPR131; G-protein coupled bile acid receptor 1; G-protein coupled bile acid receptor BG37; G-protein coupled receptor GPCR19; M-BAR; M-BARhGPCR19; membrane bile acid receptor; Membrane-type receptor for bile acids; MGC40597; TGR5; TGR5GPCR

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