Human TGR5/GPBAR1 Antibody Summary
Accession # Q8TDU6
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of TGR5/GPBAR1 in HEK293 Human Cell Line transfected with TGR5/GPBAR1 and EGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human TGR5/GPBAR1 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human TGR5/GPBAR1 Monoclonal Antibody (Catalog # MAB4286) followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B). Quadrant markers were set based on Mouse IgG2B Isotype Control (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
G protein coupled bile acid receptor 1 (GPBAR1), also called M-BAR, BG37 or TGR5, is a G-protein coupled receptor superfamily member that is a widely expressed cell surface receptor for bile acids. On macrophages, bile acid engagement of GPBAR1 suppresses phagocytosis and other functions. Extracellular portions of human GPBAR1 show approximately 75% amino acid identity with corresponding regions of mouse GPBAR1.
Citation for Human TGR5/GPBAR1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
TGR5 signalling inhibits the production of pro-inflammatory cytokines by in vitro differentiated inflammatory and intestinal macrophages in Crohn's disease.
Authors: Yoneno K, Hisamatsu T, Shimamura K, Kamada N, Ichikawa R, Kitazume M, Mori M, Uo M, Namikawa Y, Matsuoka K, Sato T, Koganei K, Sugita A, Kanai T, Hibi T
Sample Types: Whole Cells
Applications: Flow Cytometry
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