Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, CyTOF-ready

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 409522
View all TGR5/GPBAR1 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human TGR5/GPBAR1
Met1-Asn330
Accession # Q8TDU6

Specificity

Stains human TGR5/GPBAR1 transfectants but not irrelevant transfectants.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human TGR5/GPBAR1 Antibody

Detection of TGR5/GPBAR1 antibody in HEK293 Human Cell Line transfected with TGR5/GPBAR1 and EGFP antibody by Flow Cytometry.

Detection of TGR5/GPBAR1 in HEK293 Human Cell Line transfected with TGR5/GPBAR1 and EGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with (A) human TGR5/GPBAR1 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human TGR5/GPBAR1 Monoclonal Antibody (Catalog # MAB4286) followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B). Quadrant markers were set based on Mouse IgG2BIsotype Control (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

Applications for Human TGR5/GPBAR1 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 Human Cell Line transfected with TGR5/GPBAR1 and EGFP

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TGR5/GPBAR1

G protein coupled bile acid receptor 1 (GPBAR1), also called M-BAR, BG37 or TGR5, is a G-protein coupled receptor superfamily member that is a widely expressed cell surface receptor for bile acids. On macrophages, bile acid engagement of GPBAR1 suppresses phagocytosis and other functions. Extracellular portions of human GPBAR1 show approximately 75% amino acid identity with corresponding regions of mouse GPBAR1.

Long Name

Bile Acid Receptor TGR5

Alternate Names

BG37, GPBA, GPBAR1, GPCR19, GPR131, M-BAR

Entrez Gene IDs

151306 (Human); 227289 (Mouse); 338443 (Rat)

Gene Symbol

GPBAR1

UniProt

Q8TDU6

Additional TGR5/GPBAR1 Products

Product Documents for Human TGR5/GPBAR1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human TGR5/GPBAR1 Antibody

For research use only

Related Research Areas

Nutrition and Appetite

Citations for Human TGR5/GPBAR1 Antibody

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