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Human Tie-2 PE-conjugated Antibody

Catalog #: FAB3131P
12 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB3131P
Detection of Tie‑2 in HUVEC Human Cells by Flow Cytometry.
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Citations (12)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Tie-2 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Tie-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Tie‑1, recombinant mouse Tie-2, or recombinant zebrafish Tie-2 is observed.
Source
Monoclonal Mouse IgG1 Clone # 83715
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Tie‑2
Ala23-Lys745
Accession # AAA61139
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Tie-2 antibody in HUVEC Human Cells antibody by Flow Cytometry. View Larger

Detection of Tie‑2 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human Tie-2 PE-conjugated Monoclonal Antibody (Catalog # FAB3131P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Tie-2

Tie-1/Tie (tyrosine kinase with Ig and EGF homology domains 1) and Tie-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis.

Human Tie-2 cDNA encodes a 1124 amino acid (aa) residue precursor protein with an 18 residue putative signal peptide, a 727 residue extracellular domain and a 354 residue cytoplasmic domain. Two ligands, angiopoietin-1 (Ang-1) and angiopoietin-2 (Ang-2), which bind Tie-2 with high-affinity have been identified. Ang-2 has been reported to act as an antagonist for Ang-1. Mice engineered to overexpress Ang-2 or to lack Ang-1 or Tie-2 display similar angiogenesis defects.

References
  1. Partanen, J. and D.J. Dumont (1999) Curr. Top. Microbiol. Immunol. 237:159.
  2. Takakura, N. et al. (1998) Immunity 9:677.
  3. Procopio, W. et al. (1999) J. Biol. Chem. 274:30196.
Long Name
Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains 2
Entrez Gene IDs
7010 (Human); 21687 (Mouse); 89804 (Rat); 396729 (Porcine); 403714 (Canine); 102122204 (Cynomolgus Monkey); 30747 (Zebrafish)
Alternate Names
angiopoietin-1 receptor; CD202b antigen; CD202b; EC 2.7.10; EC 2.7.10.1; hTIE2; p140 TEK; soluble TIE2 variant 1; soluble TIE2 variant 2; TEK tyrosine kinase, endothelial; TEK; Tie2; Tie-2; TIE2CD202b; Tunica interna endothelial cell kinase; Tyrosine-protein kinase receptor TEK; Tyrosine-protein kinase receptor TIE-2; venous malformations, multiple cutaneous and mucosal; VMCM; VMCM1

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Citations for Human Tie-2 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Increased frequency of proangiogenic tunica intima endothelial kinase 2 (Tie2) expressing monocytes in individuals with type 2 diabetes mellitus
    Authors: M Reijrink, J van Ark, CPH Lexis, LM Visser, ME Lodewijk, ICC van der Ho, CJ Zeebregts, H van Goor, SCA de Jager, G Pasterkamp, BHR Wolffenbut, JL Hillebrand
    Cardiovascular Diabetology, 2022;21(1):72.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Circulating Tie2-Expressing Monocytes: A Potential Biomarker for Cervical Cancer
    Authors: Q Han, Q Zhang, F Ying, Z Wang, Y Zhang, L Gong, E Cai, J Qian, J Cai
    OncoTargets and Therapy, 2020;13(0):8877-8885.
    Species: Human
    Sample Types: Plasma
    Applications: Flow Cytometry
  3. MerTK expressing hepatic macrophages promote the resolution of inflammation in acute liver failure
    Authors: E Triantafyl, OT Pop, LA Possamai, A Wilhelm, E Liaskou, A Singanayag, C Bernsmeier, W Khamri, G Petts, R Dargue, SP Davies, J Tickle, M Yuksel, VC Patel, RD Abeles, Z Stamataki, SM Curbishley, Y Ma, ID Wilson, M Coen, KJ Woollard, A Quaglia, J Wendon, MR Thursz, DH Adams, CJ Weston, CG Antoniades
    Gut, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Macrophage colony-stimulating factor augments Tie2-expressing monocyte differentiation, angiogenic function, and recruitment in a mouse model of breast cancer.
    Authors: Forget M, Voorhees J, Cole S, Dakhlallah D, Patterson I, Gross A, Moldovan L, Mo X, Evans R, Marsh C, Eubank T
    PLoS ONE, 2014;9(6):e98623.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Differential sensitivity of prostate tumor derived endothelial cells to sorafenib and sunitinib.
    Authors: Fiorio Pla, Alessand, Brossa, Alessia, Bernardini, Michela, Genova, Tullio, Grolez, Guillaum, Villers, Arnaud, Leroy, Xavier, Prevarskaya, Natalia, Gkika, Dimitra, Bussolati, Benedett
    BMC Cancer, 2014;14(0):939.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. slanDCs selectively accumulate in carcinoma-draining lymph nodes and marginate metastatic cells.
    Authors: Vermi W, Micheletti A, Lonardi S, Costantini C, Calzetti F, Nascimbeni R, Bugatti M, Codazzi M, Pinter P, Schakel K, Tamassia N, Cassatella M
    Nat Commun, 2014;5(0):3029.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Human haemato-endothelial precursors: cord blood CD34+ cells produce haemogenic endothelium.
    Authors: Pelosi E, Castelli G, Martin-Padura I, Bordoni V, Santoro S, Conigliaro A, Cerio A, De Santis Puzzonia M, Marighetti P, Biffoni M, Alonzi T, Amicone L, Alcalay M, Bertolini F, Testa U, Tripodi M
    PLoS ONE, 2012;7(12):e51109.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Intermediate monocytes but not TIE2-expressing monocytes are a sensitive diagnostic indicator for colorectal cancer.
    Authors: Schauer D, Starlinger P, Reiter C, Jahn N, Zajc P, Buchberger E, Bachleitner-Hofmann T, Bergmann M, Stift A, Gruenberger T, Brostjan C
    PLoS ONE, 2012;7(9):e44450.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. The angiopoietin/Tie-2 system regulates pericyte survival and recruitment in diabetic retinopathy.
    Authors: Cai J, Kehoe O, Smith GM, Hykin P, Boulton ME
    Invest. Ophthalmol. Vis. Sci., 2008;49(5):2163-71.
    Species: Bovine
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. IFATS Series: FGF-2-induced HGF Secretion By Adipose-Derived Stromal Cells Inhibits Post-Injury Fibrogenesis Through A JNK-Dependent Mechanism.
    Authors: Suga H, Eto H, Shigeura T, Inoue K, Aoi N, Kato H, Nishimura S, Manabe I, Gonda K, Yoshimura K
    Stem Cells, 2008;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Highly efficient and feeder-free production of subculturable vascular endothelial cells from primate embryonic stem cells.
    Authors: Saeki K, Yogiashi Y, Nakahara M, Nakamura N, Matsuyama S, Koyanagi A, Yagita H, Koyanagi M, Kondo Y, Yuo A
    J. Cell. Physiol., 2008;217(1):261-80.
    Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Isolation of an adult blood-derived progenitor cell population capable of differentiation into angiogenic, myocardial and neural lineages.
    Authors: Porat Y, Porozov S, Belkin D, Shimoni D, Fisher Y, Belleli A, Czeiger D, Silverman WF, Belkin M, Battler A, Fulga V, Savion N
    Br. J. Haematol., 2006;135(5):703-14.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Isotype Controls

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Staining Reagents

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Flow Cytometry Mouse Lyse Buffer (10X)

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Flow Cytometry Staining Buffer (1X)

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