Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Bovine, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 83715
View all Tie-2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Tie‑2
Ala23-Lys745
Accession # AAA61139

Specificity

Detects human Tie-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Tie‑1, recombinant mouse Tie-2, or recombinant zebrafish Tie-2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Tie‑2 PE‑conjugated Antibody

Detection of Tie-2 antibody in HUVEC Human Cells antibody by Flow Cytometry.

Detection of Tie‑2 in HUVEC Human Cells by Flow Cytometry.

HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human Tie-2 PE-conjugated Monoclonal Antibody (Catalog # FAB3131P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of Mouse Human Tie-2 PE-conjugated Antibody by Flow Cytometry

Detection of Mouse Human Tie-2 PE-conjugated Antibody by Flow Cytometry

CSF1 up-regulates Tie2 receptor on CD14+ human monocytes.(A) CD14+ monocytes were isolated from whole blood using CD14+ microbeads. Cells were fixed and immunostained using anti-human Tie2 receptor antibody or isotype control antibody immediately following isolation (Freshly isolated) or after treated without (-CSF1) or with rhCSF1 (100 ng/ml) (+CSF1) for 24 hours. N = 10 per group and results represent the mean ± SEM of Tie2-positivity. (B) CD14+ monocytes treated with rhANG1 (100 ng/ml), rhANG2 (100 ng/ml) or a dose-response of rhCSF1 (0, 0.1, 1, 10, 100 ng/ml). ANG2 up-regulated Tie2 expression compared to ANG1 and CSF1 induces a dose-escalation of Tie2 on CD14+ monocytes. N = 10 per group and results represent the mean ± SEM of Tie2-positivity. (C) CD14+ monocytes were left untreated (Utx) or treated with rhANG2 (100 ng/ml) (ANG2), rhCSF1 (100 ng/ml) (CSF1), CSF1R neutralizing antibody alone, or pre-treated with the CSF1R Nab for 30 minutes prior to stimulation with rhCSF1 (100 ng/ml) (CSF1R NAb+CSF1) for 24 hours. ANG2- and CSF1-treatment significantly increased Tie2 expression while the CSF1R NAb abrogated this effect. N = 8 per group and results represent the mean ± SEM of Tie2-positivity by flow cytometry. (D) CD14+ monocytes were left untreated (Untreated), pre-treated with CSF1R NAb (40 µg or 80 µg) for 30 minutes then treated with rhCSF1 (100 ng/ml) (CSF1R NAb+CSF1), or with rhCSF1 (100 ng/ml) alone (CSF1) for 10 minutes. Western blot analysis indicates that the CSF1R NAb was effective at reducing Akt1 phosphorylation. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24892425), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Human Tie-2 PE-conjugated Antibody by Flow Cytometry

Detection of Mouse Human Tie-2 PE-conjugated Antibody by Flow Cytometry

CSF1 up-regulates Tie2 receptor on CD14+ human monocytes.(A) CD14+ monocytes were isolated from whole blood using CD14+ microbeads. Cells were fixed and immunostained using anti-human Tie2 receptor antibody or isotype control antibody immediately following isolation (Freshly isolated) or after treated without (-CSF1) or with rhCSF1 (100 ng/ml) (+CSF1) for 24 hours. N = 10 per group and results represent the mean ± SEM of Tie2-positivity. (B) CD14+ monocytes treated with rhANG1 (100 ng/ml), rhANG2 (100 ng/ml) or a dose-response of rhCSF1 (0, 0.1, 1, 10, 100 ng/ml). ANG2 up-regulated Tie2 expression compared to ANG1 and CSF1 induces a dose-escalation of Tie2 on CD14+ monocytes. N = 10 per group and results represent the mean ± SEM of Tie2-positivity. (C) CD14+ monocytes were left untreated (Utx) or treated with rhANG2 (100 ng/ml) (ANG2), rhCSF1 (100 ng/ml) (CSF1), CSF1R neutralizing antibody alone, or pre-treated with the CSF1R Nab for 30 minutes prior to stimulation with rhCSF1 (100 ng/ml) (CSF1R NAb+CSF1) for 24 hours. ANG2- and CSF1-treatment significantly increased Tie2 expression while the CSF1R NAb abrogated this effect. N = 8 per group and results represent the mean ± SEM of Tie2-positivity by flow cytometry. (D) CD14+ monocytes were left untreated (Untreated), pre-treated with CSF1R NAb (40 µg or 80 µg) for 30 minutes then treated with rhCSF1 (100 ng/ml) (CSF1R NAb+CSF1), or with rhCSF1 (100 ng/ml) alone (CSF1) for 10 minutes. Western blot analysis indicates that the CSF1R NAb was effective at reducing Akt1 phosphorylation. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24892425), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Tie‑2 PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: HUVEC human umbilical vein endothelial cells

Reviewed Applications

Read 2 reviews rated 4 using FAB3131P in the following applications:

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Tie-2

Tie-1/Tie (tyrosine kinase with Ig and EGF homology domains 1) and Tie-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis.

Human Tie-2 cDNA encodes a 1124 amino acid (aa) residue precursor protein with an 18 residue putative signal peptide, a 727 residue extracellular domain and a 354 residue cytoplasmic domain. Two ligands, angiopoietin-1 (Ang-1) and angiopoietin-2 (Ang-2), which bind Tie-2 with high-affinity have been identified. Ang-2 has been reported to act as an antagonist for Ang-1. Mice engineered to overexpress Ang-2 or to lack Ang-1 or Tie-2 display similar angiogenesis defects.

References

  1. Partanen, J. and D.J. Dumont (1999) Curr. Top. Microbiol. Immunol. 237:159.
  2. Takakura, N. et al. (1998) Immunity 9:677.
  3. Procopio, W. et al. (1999) J. Biol. Chem. 274:30196.

Long Name

Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains 2

Alternate Names

CD202b, TEK, Tie2

Entrez Gene IDs

7010 (Human); 21687 (Mouse); 89804 (Rat); 396729 (Porcine); 403714 (Canine); 102122204 (Cynomolgus Monkey); 30747 (Zebrafish)

Gene Symbol

TEK

UniProt

AAA61139

Additional Tie-2 Products

Product Documents for Human Tie‑2 PE‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Tie‑2 PE‑conjugated Antibody

For research use only

Citations for Human Tie‑2 PE‑conjugated Antibody

Customer Reviews for Human Tie‑2 PE‑conjugated Antibody (2)

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  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID: 20068135
    Species: Human
    Verified Customer | Posted 01/23/2015
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID: 21848603
    Species: Human
    Verified Customer | Posted 01/23/2015

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