Detects human TRAIL R4/TNFRSF10D in ELISAs and Western blots. In sandwich immunoassays, less than 5% cross-reactivity with recombinant human (rh) TRAIL R1, rhTRAIL R2, rhTRAIL R3, rhTRAIL, rhTNF‑ alpha, and rhTNF-beta is observed.
Monoclonal Mouse IgG1 Clone # 104918
Protein A or G purified from ascites
Mouse myeloma cell line NS0-derived recombinant human TRAIL R4/TNFRSF10D Ala56-His211 Accession # Q9UBN6
Supplied in a saline solution containing BSA and Sodium Azide.
Detection of TRAIL R4/TNFRSF10D in Human Whole Blood Granulocytes by Flow Cytometry.
Human whole blood granulocytes were stained with Mouse Anti-Human TRAIL R4/TNFRSF10D Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB633G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: TRAIL R4/TNFRSF10D
TRAIL-R4, also known as TRAIL-R4 alpha, Decoy Receptor 2 (DcR2) and TNFRSF10D,
is a 39-48 kDa member of the TNFR Superfamily of proteins. Mature TRAIL-R4/DcR2 is a type I
transmembrane protein 331 amino acids (aa) in length. It contains a 156 aa extracellular domain
(ECD) (aa 56-211), and a 154 aa cytoplasmic region that contains a truncated
death domain. The presence of this
truncation is not believed to allow for a positive signal for the initiation of
apoptosis. This has led to its description
as a "decoy receptor", suggesting that its function is to simply sequester and decrease
the amount of TRAIL available for binding to the two apoptosis-inducing TRAIL
receptors, DR4 and DR5. At this time,
this view would appear to be unlikely, as it now seems that DcR2 actually
complexes with DR5/TRAIL-R2, blocking the activation of Caspase-8, a first step
in the initiation of apoptosis. In
addition, in a ligand-dependent manner, the presence of DcR2 has also been
associated with the increased survival of select leukocytes. Notably, an isoform variant for TRAIL-R4
(called TRAIL-R4 beta ) has been reported that shows an absence of aa 87-124, a
deletion that would render it incapable of binding to TRAIL. Cells reported to express TRAIL-R4 are
varied, and include eosinophils, NKT cells, CD8+ (but not CD4+) T cells, colonic
columnar epithelium, neutrophils, endometrial columnar epithelium, testicular
spermatids and testosterone-secreting Leydig cells, and multiple tumor cell
types. A rodent counterpart to TRAIL-R4
has not been reported.
TRAIL Receptor 4
Entrez Gene IDs:
CD264 antigen; CD264; DcR2; DCR2decoy with truncated death domain; Decoy receptor 2; TNF receptor-related receptor for TRAIL; TNF-related apoptosis-inducing ligand receptor 4; TNFRSF10D; TRAIL R4; TRAILR4; TRAIL-R4; TRAILR4TRAIL receptor 4; TRUNDDTRAIL receptor with a truncated death domain; tumor necrosis factor receptor superfamily member 10D; tumor necrosis factor receptor superfamily, member 10d, decoy with truncateddeath domain
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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