Human Transcobalamin II Alexa Fluor™ Plus 680‑conjugated Antibody

R&D Systems | Catalog # FAB7895AFP680

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Alexa Fluor Plus 680 (Excitation = 687 nm, Emission = 704 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 874420
View all Transcobalamin II Primary Antibodies »

Product Specifications

Specificity

Detects human Transcobalamin II in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human Transcobalamin II Alexa Fluor™ Plus 680‑conjugated Antibody

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Transcobalamin II

Transcobalamin II, also known as TCN2, TC-2 or TC, is a 42-44 kDa, monomeric, secreted member of the eukaryotic cobalamin transport family of molecules. It is a key player in the transport and absorption of VitB12. TCN1 is a salivary gland protein that binds oral/dietary B12. Once in the stomach, B12 is transferred to IF/Intrinsic Factor, and this complex remains intact during passage through the small intestine. In the distal ileum, the IF:VitB12 complex is internalized following binding to cubam on enterocyte membranes. Once internalized, VitB12 is processed and re-expressed on MRP1 on the enterocyte's basal surface. Here it is transferred to TCN-2, a binding protein secreted by endothelial cells that accounts for the transport of anywhere from 10-30% of circulating VitB12/cobalamin. The TCN2:VitB12 complex is ultimately internalized by cells expressing a membrane-bound, 58 kDa TCN2 receptor termed TCblR. Once internalized, VitB12 is released and converted into cofactors that are involved in methionine biosynthesis. Human TCN2 is synthesized as a 427 amino acid (aa) precursor that contains an 18 aa signal sequence, plus a 409 aa mature region that binds one VitB12 molecule. There are three potential splice variants of TCN2. One contains a Trp substitution for aa 116-143, a second possesses an Ile substitution for aa 336-427, and a third shows a deletion of aa 143-193. An Arg-to-Pro polymorphism at position 259 correlates with statistically elevated circulating levels of TCN2. Over aa 19-427, human TCN2 shares 72% aa sequence identity with mouse TCN2.

Alternate Names

TC-2, TCII, TCN2, Vitamin B12-binding Protein 2

Entrez Gene IDs

6948 (Human); 21452 (Mouse); 64365 (Rat)

Gene Symbol

TCN2

UniProt

P20062

Additional Transcobalamin II Products

Product Documents for Human Transcobalamin II Alexa Fluor™ Plus 680‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Transcobalamin II Alexa Fluor™ Plus 680‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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