Human Trappin-2/Elafin Alexa Fluor® 488-conjugated Antibody

Catalog #: FAB1747G Datasheet / COA / SDS

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FAB1747G-100UG

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Human Trappin-2/Elafin Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects the full length form (amino acids 23‑117) and the trypsin-processed form (amino acids 61‑117) of human Trappin‑2/Elafin in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human SLPI is observed.

Source

Monoclonal Mouse IgG1 Clone # 257729

Purification

Protein A or G purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Trappinâ€‘2/Elafin
Ala23-Gln117
Accession # P19957

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Trappin-2/Elafin

Trappin-2 is the human member of the trappin gene family that contains SLPI (1). Trappin-2 consists of an N-terminal transglutaminase substrate domain (residues
23‑60) and a C-terminal four-disulfide core or whey acidic protein (WAP) domain (residues 72‑117). Elafin or ESI (elastase-specific inhibitor) and SKALP (skin-derived anti-leucoproteinase) are alternative names for Trappin-2 and reflect its protease targets. However, elafin and SKALP sometimes correspond only to the processed form that contains the C-terminal WAP domain of the molecule, which can be isolated naturally. The recombinant human Trappin-2 corresponds to the full-length form (residues 23‑117), which migrates as two protein bands under SDS-PAGE due to an unidentified mechanism. In addition to its ability to inhibit human neutrophil elastase, it can also be used as a substrate for transglutaminases.

Long Name

Elastase Inhibitor, Transglutaminase Substrate, Serine Protease

Entrez Gene IDs

5266 (Human)

Alternate Names

cementoin; Elafin; Elastase-specific inhibitor; ESI; ESIWAP four-disulfide core domain protein 14; Peptidase inhibitor 3; peptidase inhibitor 3, skin-derived; PI3; PI-3; pre-elafin; protease inhibitor 3, skin-derived (SKALP); Protease inhibitor WAP3; SKALP; SKALPELAFIN; Skin-derived antileukoproteinase; Trappin2; Trappin-2; WAP four-disulfide core domain 14; WAP3MGC13613; WFDC14elafin