Human TREM-1 Quantikine ELISA Kit DTRM10C: R&D Systems

Human TREM-1 Quantikine ELISA Kit

(6 citations)   
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Saliva (50 uL)
  • Sensitivity
    15.2 pg/mL
  • Assay Range
    62.5 - 4,000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva)
  • Specificity
    Natural and recombinant human TREM-1.
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC210, Quantikine Immunoassay Control Set 867 Human TREM-1 - Please Inquire
Product Summary
The Quantikine Human TREM-1 Immunoassay is a 4.5 hour solid phase ELISA designed to measure TREM-1 levels in cell culture supernates, cell lysates, serum, plasma, and saliva. It contains NS0-expressed recombinant human TREM-1 and antibodies raised against the recombinant protein. Results obtained for naturally occurring human TREM-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine Human TREM-1 Immunoassay standards. These results indicate that this kit can be used to determine relative mass values for natural human TREM-1.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean4791226221751112862254
Standard Deviation16.841.572.12557.790.7
CV%3.53.43.34.94.54

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020404040
Mean4101212242443912152354
Standard Deviation21.350.787.932.770.4166
CV%5.24.23.67.45.87.1

Recovery

The recovery of human TREM-1 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 102 94-112
Cell Lysis Buffer (n=4) 88 78-96
EDTA Plasma (n=4) 99 92-105
Heparin Plasma (n=4) 96 87-104
Serum (n=4) 97 84-107
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of human TREM-1 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human TREM-1 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: TREM-1
The TREM family of receptors regulates the activity of various cell types of the immune system including neutrophils, monocyte/macrophages, microglia, and dendritic cells. The receptors are members of the immunoglobulin superfamily, characterized structurally by a single V-set immunoglobulin (Ig) domain and a short cytoplasmic tail. TREMs associate with the DAP12 signal adapter, an immunoreceptor tyrosine-based activation motif- (ITAM)-containing protein that is phosphorylated and transduces signals when activated.
    • Long Name
      Triggering Receptor Expressed on Myeloid Cells 1
    • Entrez Gene IDs
      54210 (Human); 58217 (Mouse);
    • Alternate Names
      CD354 antigen; CD354; TREM1; TREM-1; TREM-1Triggering receptor expressed on monocytes 1; triggering receptor expressed on myeloid cells 1; triggering-receptor TREM1;
    Related Research Areas
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    6 Citations: Showing 1 - 6
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    Species
    Sample Type
    1. TREM-1 SNP rs2234246 regulates TREM-1 protein and mRNA levels and is associated with plasma levels of L-selectin
      Authors: AA Aldasoro A, S Dadé, M Stathopoul, M Derive, N Coumba Ndi, T Xie, C Masson, S Gibot, S Visvikis-S
      PLoS ONE, 2017;12(8):e0182226.
      Species: Human
      Sample Type: Serum
    2. Decoy receptor 3 levels in peripheral blood predict outcomes of acute respiratory distress syndrome.
      Authors: Chen CY, Yang KY, Chen MY, Chen HY, Lin MT, Lee YC, Perng RP, Hsieh SL, Yang PC, Chou TY
      Am. J. Respir. Crit. Care Med., 2009;180(8):751-60.
      Species: Human
      Sample Type: Plasma
    3. Correlation of serum-soluble triggering receptor expressed on myeloid cells-1 with clinical disease activity in inflammatory bowel disease.
      Authors: Park JJ, Cheon JH, Kim BY, Kim DH, Kim ES, Kim TI, Lee KR, Kim WH
      Dig. Dis. Sci., 2009;54(7):1525-31.
      Species: Human
      Sample Type: Serum
    4. Intervention of an inflammation amplifier, triggering receptor expressed on myeloid cells 1, for treatment of autoimmune arthritis.
      Authors: Murakami Y, Akahoshi T, Aoki N, Toyomoto M, Miyasaka N, Kohsaka H
      Arthritis Rheum., 2009;60(6):1615-23.
      Species: Human
      Sample Type: Serum
    5. Increased levels of soluble triggering receptor expressed on myeloid cells-1 in patients with acute pancreatitis.
      Authors: Yasuda T, Takeyama Y, Ueda T, Shinzeki M, Sawa H, Takahiro N, Kamei K, Ku Y, Kuroda Y, Ohyanagi H
      Crit. Care Med., 2008;36(7):2048-53.
      Species: Human
      Sample Type: Serum
    6. TREM-1 expression in tumor-associated macrophages and clinical outcome in lung cancer.
      Authors: Ho CC, Liao WY, Wang CY, Lu YH, Huang HY, Chen HY, Chan WK, Chen HW, Yang PC
      Am. J. Respir. Crit. Care Med., 2007;177(7):763-70.
      Species: Human
      Sample Type: Cell Culture Supernates
    ELISA Controls
    Description Application Cat# Citations Images  

    Quantikine Immunoassay Control Set 867 Human TREM-1

    Ctrl QC210
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    Supplemental ELISA Products
    Description Application Cat# Citations Images  

    Quantikine ELISA Wash Buffer 1

    ELISA WA126 6
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    Sample Diluent Concentrate 2 (2X)

    ELISA DYC002 2
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