Human TREM-1 Quantikine ELISA Kit

Catalog # Availability Size / Price Qty
DTRM10C
Control Products Available
Human TREM-1 Simple Plex Assay Linearity
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Product Details
Citations (14)
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Human TREM-1 Quantikine ELISA Kit Summary

Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Saliva (50 uL)
Sensitivity
15.2 pg/mL
Assay Range
62.5 - 4,000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva)
Specificity
Natural and recombinant human TREM-1.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human TREM-1 Immunoassay is a 4.5 hour solid phase ELISA designed to measure TREM-1 levels in cell culture supernates, cell lysates, serum, plasma, and saliva. It contains NS0-expressed recombinant human TREM-1 and antibodies raised against the recombinant protein. Results obtained for naturally occurring human TREM-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine Human TREM-1 Immunoassay standards. These results indicate that this kit can be used to determine relative mass values for natural human TREM-1.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 479 1226 2217 511 1286 2254
Standard Deviation 16.8 41.5 72.1 25 57.7 90.7
CV% 3.5 3.4 3.3 4.9 4.5 4

Recovery

The recovery of human TREM-1 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 102 94-112
Cell Lysis Buffer (n=4) 88 78-96
EDTA Plasma (n=4) 99 92-105
Heparin Plasma (n=4) 96 87-104
Serum (n=4) 97 84-107

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of human TREM-1 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human TREM-1 Simple Plex Assay Linearity
Human TREM-1 ELISA Linearity

Scientific Data

Human TREM-1 ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TREM-1

The TREM family of receptors regulates the activity of various cell types of the immune system including neutrophils, monocyte/macrophages, microglia, and dendritic cells. The receptors are members of the immunoglobulin superfamily, characterized structurally by a single V-set immunoglobulin (Ig) domain and a short cytoplasmic tail. TREMs associate with the DAP12 signal adapter, an immunoreceptor tyrosine-based activation motif- (ITAM)-containing protein that is phosphorylated and transduces signals when activated.

Long Name:
Triggering Receptor Expressed on Myeloid Cells 1
Entrez Gene IDs:
54210 (Human); 58217 (Mouse)
Alternate Names:
CD354 antigen; CD354; TREM1; TREM-1; TREM-1Triggering receptor expressed on monocytes 1; triggering receptor expressed on myeloid cells 1; triggering-receptor TREM1
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human TREM-1 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Oxidative stress-induced endothelial dysfunction and decreased vascular nitric oxide in COVID-19 patients
    Authors: V Montiel, I Lobysheva, L Gérard, M Vermeersch, D Perez-Morg, T Castelein, JB Mesland, P Hantson, C Collienne, D Gruson, MA van Dievoe, A Persu, C Beauloye, M Dechamps, L Belkhir, A Robert, M Derive, PF Laterre, AHJ Danser, X Wittebole, JL Balligand
    EBioMedicine, 2022;77(0):103893.
    Species: Human
    Sample Types: Plasma
  2. Increased sTREM-1 plasma concentrations are associated with poor clinical outcomes in patients with COVID-19
    Authors: AH de Nooijer, I Grondman, S Lambden, EJ Kooistra, NA Janssen, M Kox, P Pickkers, LA Joosten, F van de Vee, M Derive, S Gibot, MG Netea
    Bioscience Reports, 2021;0(0):.
    Species: Human
    Sample Types: Plasma
  3. Diagnostic value of serum soluble triggering expressed receptor on myeloid cells 1 (sTREM-1) in suspected sepsis: a meta-analysis
    Authors: W Chang, F Peng, SS Meng, JY Xu, Y Yang
    BMC Immunol., 2020;21(1):2.
    Species: Human
    Sample Types: Serum
  4. HIV and HCV augments inflammatory responses through increased TREM-1 expression and signaling in Kupffer and Myeloid cells
    Authors: J Hyun, RS McMahon, AL Lang, JS Edwards, AD Badilla, ME Greene, GW Stone, S Pallikkuth, M Stevenson, DM Dykxhoorn, S Kottilil, S Pahwa, E Thomas
    PLoS Pathog., 2019;15(7):e1007883.
    Species: Human
    Sample Types: Cell Lysates
  5. Inhibition of Triggering Receptor Expressed on Myeloid Cell-1 Alleviates Acute Gouty Inflammation
    Authors: Y He, Q Yang, X Wang, A Jia, W Xie, J Zhou
    Mediators Inflamm., 2019;2019(0):5647074.
    Species: Human
    Sample Types: Plasma
  6. Pleiotropy of ABO gene: correlation of rs644234 with E-selectin and lipid levels
    Authors: AA Arguinano, NC Ndiaye, C Masson, S Visvikis-S
    Clin. Chem. Lab. Med., 2018;0(0):.
    Species: Human
    Sample Types: Serum
  7. Role of TREM-1 in pulmonary tuberculosis patients- analysis of serum soluble TREM-1 levels
    Authors: JY Feng, WJ Su, SW Pan, YC Yeh, YY Lin, NJ Chen
    Sci Rep, 2018;8(1):8223.
    Species: Human
    Sample Types: Serum
  8. Urine TREM-1 as a marker of urinary tract infection in children
    Authors: E Sierra-Dia, A Bravo Cuél, PC Ortiz Laza, M García Gut, HF Georgina, R Anaya Prad
    J. Int. Med. Res., 2017;45(2):631-638.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. TREM-1 SNP rs2234246 regulates TREM-1 protein and mRNA levels and is associated with plasma levels of L-selectin
    Authors: AA Aldasoro A, S Dadé, M Stathopoul, M Derive, N Coumba Ndi, T Xie, C Masson, S Gibot, S Visvikis-S
    PLoS ONE, 2017;12(8):e0182226.
    Species: Human
    Sample Types: Serum
  10. Intervention of an inflammation amplifier, triggering receptor expressed on myeloid cells 1, for treatment of autoimmune arthritis.
    Authors: Murakami Y, Akahoshi T, Aoki N, Toyomoto M, Miyasaka N, Kohsaka H
    Arthritis Rheum., 2009;60(6):1615-23.
    Species: Human
    Sample Types: Serum
  11. Decoy receptor 3 levels in peripheral blood predict outcomes of acute respiratory distress syndrome.
    Authors: Chen CY, Yang KY, Chen MY, Chen HY, Lin MT, Lee YC, Perng RP, Hsieh SL, Yang PC, Chou TY
    Am. J. Respir. Crit. Care Med., 2009;180(8):751-60.
    Species: Human
    Sample Types: Plasma
  12. Correlation of serum-soluble triggering receptor expressed on myeloid cells-1 with clinical disease activity in inflammatory bowel disease.
    Authors: Park JJ, Cheon JH, Kim BY, Kim DH, Kim ES, Kim TI, Lee KR, Kim WH
    Dig. Dis. Sci., 2009;54(7):1525-31.
    Species: Human
    Sample Types: Serum
  13. Increased levels of soluble triggering receptor expressed on myeloid cells-1 in patients with acute pancreatitis.
    Authors: Yasuda T, Takeyama Y, Ueda T, Shinzeki M, Sawa H, Takahiro N, Kamei K, Ku Y, Kuroda Y, Ohyanagi H
    Crit. Care Med., 2008;36(7):2048-53.
    Species: Human
    Sample Types: Serum
  14. TREM-1 expression in tumor-associated macrophages and clinical outcome in lung cancer.
    Authors: Ho CC, Liao WY, Wang CY, Lu YH, Huang HY, Chen HY, Chan WK, Chen HW, Yang PC
    Am. J. Respir. Crit. Care Med., 2007;177(7):763-70.
    Species: Human
    Sample Types: Cell Culture Supernates

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