Human TrkC Alexa Fluor® 647-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB3731R-100UG
Detection of TrkC in Human TrkC transfectedcells by Flow Cytometry.
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Human TrkC Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human TrkC in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) TrkA, rhTrkB, or recombinant mouse TrkC is observed.
Source
Monoclonal Mouse IgG1 Clone # 75213
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TrkC
Cys32-Asp428
Accession # Q16288
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of TrkC in Human TrkC transfectedcells by Flow Cytometry. View Larger

Detection of TrkC in Human TrkC transfectedcells by Flow Cytometry. Human TrkC transfected Baf/3 cells (A),or (B) irrelevant transfectants and eGFP, were stained with Mouse Anti-Human TrkCAlexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB3731R).Quadrants were set based on Mouse Monoclonal IgG2B Alexa Fluor® 647-conjugatedisotype control antibody (Catalog # IC0041R, data not shown). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TrkC

The neurotrophins, including NGF, BDNF, NT-3 and NT-4/5, constitute a group of structurally related, secreted proteins that play an important role in the development and function of the nervous system. The biological activities of the neurotrophins are mediated by binding to and activating two unrelated receptor types: the p75 neurotrophin receptor (p75NTR) and the Trk family of receptor tyrosine kinases (1, 2). P75NTR is a member of the tumor necrosis factor receptor superfamily (TNFRSF) and has been designated TNFRSF16. It binds all neurotrophins with low-affinity to transduce cellular signaling pathways that synergize or antagonize those activated by the Trk receptors. Three Trk family proteins, TrkA, TrkB and TrkC, exhibiting different ligand specificities, have been identified. TrkA binds NGF and NT‑3, TrkB binds BDNF, NT-3 and NT-4/5, and TrkC only binds NT-3 (1, 2). All Trk family proteins share a conserved, complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain (3). Natural splice variants of the different Trks, lacking the first cysteine-rich domain, the first and second or all three of the leucine-rich motifs, or the tyrosine kinase domain, have been described (4). At the protein sequence level, Trks are highly conserved between species with the extracellular domains of human and mouse TrkC's showing 94% amino acid sequence identity (5). The proteins also exhibit cross-species activity. The primary location of TrkC expression is in the nervous system and, specifically, in regions of the CNS. Low level TrkC expression has also been observed in a wide variety of tissues outside the nervous system (6).

References
  1. Huang, E.J. and L.F. Reichardt. (2003) Annu. Rev. Biochem. 72:(epub ahead of print).
  2. Dechant, G. (2001) Cell Tissue Res. 305:229.
  3. Schneider, R. and M. Schweiger (1991) Oncogene 6:1807.
  4. Ninkina, N. et al. (1997) J. Biol. Chem. 272:13019.
  5. Menn, B. et al. (1998) J. Comp. Neurol. 401:47.
  6. Shelton, D. et al. (1995) J. Neurosci. 15:477.
Long Name
Neurotrophic Tyrosine Kinase Receptor C
Entrez Gene IDs
4916 (Human); 18213 (Mouse); 29613 (Rat)
Alternate Names
EC 2.7.10; EC 2.7.10.1; ETS related protein-neurotrophic receptor tyrosine kinase fusion protein; ETV6-NTRK3 fusion; gp145(trkC); GP145-TrkC; Neurotrophic tyrosine kinase receptor type 3; neurotrophic tyrosine kinase, receptor, type 3; NT 3 receptor; NT-3 growth factor receptor; NTRK3; TrkC tyrosine kinase; TrkC; trk-C; TRKCneurotrophin 3 receptor; tyrosine kinase receptor C

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