Human TSPAN12 Alexa Fluor® 647-conjugated Antibody

  • Species Reactivity
  • Specificity
    Stains human TSPAN12 transfectants but not irrelevant transfectants in flow cytometry. 
  • Source
    Monoclonal Mouse IgG2B Clone # 921938
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with human TSPAN12
    Accession # O95859
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 647
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of TSPAN12 in HEK293 Human Cell Line Transfected with Human TSPAN12 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human TSPAN12 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human TSPAN12 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB8910R). Quadrant markers were set based on control antibody staining (Catalog # IC0041R). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TSPAN12
TSPAN12 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. They mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. TSPAN12 plays a central role in retinal vascularization by regulating norrin signal transduction. TSPAN12 acts in concert with norrin to promote FZD4 multimerization and subsequent activation of FZD4, leading to promote accumulation of beta-catenin and stimulate LEF/TCF-mediated transcriptional programs. Defects in TSPAN12 are the cause of vitreoretinopathy exudative type 5, a disorder of the retinal vasculature characterized by an abrupt cessation of growth of peripheral capillaries, leading to an avascular peripheral retina.
  • Long Name:
    Tetraspanin 12
  • Entrez Gene IDs:
    23554 (Human); 269831 (Mouse); 362326 (Rat)
  • Alternate Names:
    EVR5; Leukemia-associated protein with a CXXC domain; methylcytosine dioxygenase TET1; NET2; NET-2; ten-eleven translocation-1; tetraspanin-12; TM4SF12
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Alexa Fluor® 647-conjugated Isotype Control

Ctrl IC0041R
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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