Human TSPAN12 Alexa Fluor® 647-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB8910R-025
Detection of TSPAN12 in HEK293 Human Cell Line Transfected with Human TSPAN12 and eGFP  by Flow Cytometry.
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Human TSPAN12 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Stains human TSPAN12 transfectants but not irrelevant transfectants in flow cytometry. 
Source
Monoclonal Mouse IgG2B Clone # 921938
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human TSPAN12
Met1-Leu305
Accession # O95859
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647

Applications

Recommended Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of TSPAN12 in HEK293 Human Cell Line Transfected with Human TSPAN12 and eGFP  by Flow Cytometry. View Larger

Detection of TSPAN12 in HEK293 Human Cell Line Transfected with Human TSPAN12 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human TSPAN12 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human TSPAN12 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB8910R). Quadrant markers were set based on control antibody staining (Catalog # IC0041R). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TSPAN12

TSPAN12 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. They mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. TSPAN12 plays a central role in retinal vascularization by regulating norrin signal transduction. TSPAN12 acts in concert with norrin to promote FZD4 multimerization and subsequent activation of FZD4, leading to promote accumulation of beta-catenin and stimulate LEF/TCF-mediated transcriptional programs. Defects in TSPAN12 are the cause of vitreoretinopathy exudative type 5, a disorder of the retinal vasculature characterized by an abrupt cessation of growth of peripheral capillaries, leading to an avascular peripheral retina.

Long Name
Tetraspanin 12
Entrez Gene IDs
23554 (Human); 269831 (Mouse); 362326 (Rat)
Alternate Names
EVR5; Leukemia-associated protein with a CXXC domain; methylcytosine dioxygenase TET1; NET2; NET-2; ten-eleven translocation-1; tetraspanin-12; TM4SF12; TSPAN12

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