Human TSPAN12 Antibody

Catalog # Availability Size / Price Qty
MAB8910
MAB8910-SP
Detection of TSPAN12 in HEK293 Human Cell Line Transfected with Human TSPAN12 and eGFP by Flow Cytometry.
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Human TSPAN12 Antibody Summary

Species Reactivity
Human
Specificity
Detects human TSPAN12 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 921938
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human TSPAN12
Met1-Leu305
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of TSPAN12 in HEK293 Human Cell Line Transfected with Human TSPAN12 and eGFP by Flow Cytometry. View Larger

Detection of TSPAN12 in HEK293 Human Cell Line Transfected with Human TSPAN12 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human TSPAN12 and eGFP was stained with either (A) Mouse Anti-Human TSPAN12 Monoclonal Antibody (Catalog # MAB8910) or (B) Mouse IgG2B Isotype Control (Catalog # MAB004) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Immunocytochemistry TSPAN12 in HeLa Human Cell Line. View Larger

TSPAN12 in HeLa Human Cell Line. TSPAN12 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human TSPAN12 Monoclonal Antibody (Catalog # MAB8910) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TSPAN12

TSPAN12 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. They mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. TSPAN12 plays a central role in retinal vascularization by regulating norrin signal transduction. TSPAN12 acts in concert with norrin to promote FZD4 multimerization and subsequent activation of FZD4, leading to promote accumulation of beta-catenin and stimulate LEF/TCF-mediated transcriptional programs. Defects in TSPAN12 are the cause of vitreoretinopathy exudative type 5, a disorder of the retinal vasculature characterized by an abrupt cessation of growth of peripheral capillaries, leading to an avascular peripheral retina.

Long Name
Tetraspanin 12
Entrez Gene IDs
23554 (Human); 269831 (Mouse); 362326 (Rat)
Alternate Names
EVR5; Leukemia-associated protein with a CXXC domain; methylcytosine dioxygenase TET1; NET2; NET-2; ten-eleven translocation-1; tetraspanin-12; TM4SF12; TSPAN12

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Mouse IgG2B Antibody

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