Human TSPAN7 Alexa Fluor® 647-conjugated Antibody

  
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  • Species Reactivity
    Human
  • Specificity
    Stains human TSPAN7 transfectants but not irrelevant transfectants in flow cytometry.
  • Source
    Monoclonal Mouse IgG2A Clone # 482618
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    HEK293 human embryonic kidney cell line transfected with human TSPAN7
    Accession # P41732
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 647
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of TSPAN7 in HEK Human Cell Line Transfected with Human TSPAN7 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human TSPAN7 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human TSPAN7 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB9179R). Quadrant markers were set based on control antibody staining (Catalog # IC003R). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TSPAN7
Tetraspanin-7 (aka TSPAN7,  TALLA-1, TM4SF2 and CD231) is a 249 aminoacids (aa) multi-pass membrane protein.  Tetraspanin proteins regulate morphology, signaling and trafficking processes by interaction and association with proteins in tetraspanin enriched microdomains (TEMs). It has been proposed that tetraspanins interact with integrins to affect cell migration, probably by modulation or compartmentalization of integrin signaling. TSPAN7 has been found to be upregulated in myeloma development and described as a prognostic marker of renal cell carcinoma. Mutations in TSPAN7 are also implicated in some forms of X-linked intellectual disability.
  • Long Name:
    Tetraspanin 7
  • Entrez Gene IDs:
    7102 (Human); 21912 (Mouse); 363447 (Rat)
  • Alternate Names:
    A15; A15CCG-B7; CCG-B7; CD231 antigen; CD231; Cell surface glycoprotein A15; DXS1692E; Membrane component chromosome X surface marker 1; mental retardation, X-linked 58; MRX58; MXS1; MXS1T-cell acute lymphoblastic leukemia associated antigen 1; TALLA-1; T-cell acute lymphoblastic leukemia-associated antigen 1; tetraspanin 7; tetraspanin-7; TM4SF2; TM4SF2b; TM4SF2tetraspanin protein; transmembrane 4 superfamily 2b; Transmembrane 4 superfamily member 2X chromosome, surface marker 1; transmembrane protein A15; TSPAN7; tspan-7
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Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A Alexa Fluor® 647-conjugated Isotype Control

Ctrl IC003R
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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