Human TSPAN7 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Stains human TSPAN7 transfectants but not irrelevant transfectants in flow cytometry.
  • Source
    Monoclonal Mouse IgG2A Clone # 482618
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    HEK293 human embryonic kidney cell line transfected with human TSPAN7
    Accession # P41732
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of TSPAN7 in HEK293 Human Cell Line Transfected with Human TSPAN7 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line either transfected with (A) human TSPAN7 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human TSPAN7 Monoclonal Antibody (Catalog # MAB9179) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TSPAN7
Tetraspanin-7 (aka TSPAN7,  TALLA-1, TM4SF2 and CD231) is a 249 aminoacids (aa) multi-pass membrane protein.  Tetraspanin proteins regulate morphology, signaling and trafficking processes by interaction and association with proteins in tetraspanin enriched microdomains (TEMs). It has been proposed that tetraspanins interact with integrins to affect cell migration, probably by modulation or compartmentalization of integrin signaling. TSPAN7 has been found to be upregulated in myeloma development and described as a prognostic marker of renal cell carcinoma. Mutations in TSPAN7 are also implicated in some forms of X-linked intellectual disability.
  • Long Name:
    Tetraspanin 7
  • Entrez Gene IDs:
    7102 (Human); 21912 (Mouse); 363447 (Rat)
  • Alternate Names:
    A15; A15CCG-B7; CCG-B7; CD231 antigen; CD231; Cell surface glycoprotein A15; DXS1692E; Membrane component chromosome X surface marker 1; mental retardation, X-linked 58; MRX58; MXS1; MXS1T-cell acute lymphoblastic leukemia associated antigen 1; TALLA-1; T-cell acute lymphoblastic leukemia-associated antigen 1; tetraspanin 7; tetraspanin-7; TM4SF2; TM4SF2b; TM4SF2tetraspanin protein; transmembrane 4 superfamily 2b; Transmembrane 4 superfamily member 2X chromosome, surface marker 1; transmembrane protein A15; TSPAN7; tspan-7
Related Research Areas
Isotype Controls
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Mouse IgG2A Isotype Control

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Secondary Antibodies
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Mouse IgG HRP-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

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Goat Anti-Mouse IgG Biotinylated Antibody

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Mouse IgG Antibody

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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

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Rat Anti-Mouse IgG2A PE-conjugated Antibody

Flow F0129 1  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

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Mouse IgG2A Antibody

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Rat Anti-Mouse IgG2A APC-conjugated Antibody

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Rat Anti-Mouse IgG2A PerCP-conjugated Antibody

Flow F0131  
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