Human TSPAN7 Antibody

Catalog # Availability Size / Price Qty
MAB9179-100
MAB9179-SP
Detection of  TSPAN7 in HEK293 Human Cell Line Transfected with Human TSPAN7 and eGFP by  Flow Cytometry.
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Human TSPAN7 Antibody Summary

Species Reactivity
Human
Specificity
Stains human TSPAN7 transfectants but not irrelevant transfectants in flow cytometry.
Source
Monoclonal Mouse IgG2A Clone # 482618
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
HEK293 human embryonic kidney cell line transfected with human TSPAN7
Accession # P41732
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of  TSPAN7 in HEK293 Human Cell Line Transfected with Human TSPAN7 and eGFP by  Flow Cytometry. View Larger

Detection of TSPAN7 in HEK293 Human Cell Line Transfected with Human TSPAN7 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line either transfected with (A) human TSPAN7 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human TSPAN7 Monoclonal Antibody (Catalog # MAB9179) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TSPAN7

Tetraspanin-7 (aka TSPAN7,  TALLA-1, TM4SF2 and CD231) is a 249 aminoacids (aa) multi-pass membrane protein.  Tetraspanin proteins regulate morphology, signaling and trafficking processes by interaction and association with proteins in tetraspanin enriched microdomains (TEMs). It has been proposed that tetraspanins interact with integrins to affect cell migration, probably by modulation or compartmentalization of integrin signaling. TSPAN7 has been found to be upregulated in myeloma development and described as a prognostic marker of renal cell carcinoma. Mutations in TSPAN7 are also implicated in some forms of X-linked intellectual disability.

Long Name
Tetraspanin 7
Entrez Gene IDs
7102 (Human); 21912 (Mouse); 363447 (Rat)
Alternate Names
A15; A15CCG-B7; CCG-B7; CD231 antigen; CD231; Cell surface glycoprotein A15; DXS1692E; Membrane component chromosome X surface marker 1; mental retardation, X-linked 58; MRX58; MXS1; MXS1T-cell acute lymphoblastic leukemia associated antigen 1; TALLA-1; T-cell acute lymphoblastic leukemia-associated antigen 1; tetraspanin 7; tetraspanin-7; TM4SF2; TM4SF2b; TM4SF2tetraspanin protein; transmembrane 4 superfamily 2b; Transmembrane 4 superfamily member 2X chromosome, surface marker 1; transmembrane protein A15; TSPAN7; tspan-7

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Mouse IgG2A Isotype Control

Ctrl

Reconstitution Buffers

Secondary Antibodies

Mouse IgG2A Antibody

Mouse IgG2A Antibody

Reviews for Human TSPAN7 Antibody

There are currently no reviews for this product. Be the first to review Human TSPAN7 Antibody and earn rewards!

Have you used Human TSPAN7 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review