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Human TTK Alexa Fluor® 488-conjugated Antibody

Catalog #: AF6028G Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF6028G-100UG

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TTK Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human TTK in Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human TTK
Ser281-Leu481
Accession # P33981
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: TTK

TTK (Dual specificity Thr/Tyr kinase; also PYT and hMps1) is a 97-105 kDa member of the Ser/Thr protein kinase family of enzymes. It is expressed in rapidly proliferating tissues and cell lines, and participates in cell cycle regulation. In particular, it directs chromosome alignment, centrosome duplication, and spindle assembly checkpoint formation. Via phosphorylation, TTK is known to regulate p53, CDCA8 and TACC2. Human TTK is 857 amino acids (aa) in length and contains one protein kinase domain (aa 525-791). Activation occurs following homodimerization and autophosphorylation on Thr675, Thr676 and Thr686.

Long Name
TTK Protein Kinase
Entrez Gene IDs
7272 (Human); 22137 (Mouse); 315852 (Rat)
Alternate Names
CT96; dual specificity protein kinase TTK; EC 2.7.12.1; ESK; FLJ38280; monopolar spindle 1-like 1; Mps1; MPS1L1; MPS1L1PYTcancer/testis antigen 96; phosphotyrosine picked threonine kinase; Phosphotyrosine picked threonine-protein kinase; PYT; TTK protein kinase; TTK

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