Detection of Sheep IgGControl by Flow Cytometry C2C12mouse myoblast cell line was stained with Sheep Anti-Mouse AHRAlexa Fluor® 488-conjugated Antigen Affinity-purified PolyclonalAntibody (Catalog # IC6697G,filled histogram) or Sheep IgG Alexa Fluor® 488-conjugated IsotypeControl Antibody (Catalog # IC016G, open histogram). To facilitate intracellularstaining, cells were fixed with Flow Cytometry Fixation Buffer(Catalog # FC004)and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I|
(Catalog # FC005).View our protocol for StainingIntracellular Molecules.
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.