Sheep IgG Alexa Fluor® 488-conjugated Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Sheep IgG Control by Flow Cytometry C2C12 mouse myoblast cell line was stained with Sheep Anti-Mouse AHR Alexa Fluor® 488-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC6697G, filled histogram) or Sheep IgG Alexa Fluor® 488-conjugated Isotype Control Antibody (Catalog # IC016G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Citations for Sheep IgG Alexa Fluor® 488-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Profound inhibition of CD73-dependent formation of anti-inflammatory adenosine in B cells of SLE patients
Authors: J Hesse, M Siekierka-, B Steckel, C Alter, M Schallehn, N Honke, ML Schniering, M Wippich, R Braband, M Schneider, H Surowy, D Wieczorek, J Schrader, G Pongratz
Applications: Flow Cytometry
Multiscale cytometry and regulation of 3D cell cultures on a chip
Authors: S Sart, RF Tomasi, G Amselem, CN Baroud
Nat Commun, 2017;8(1):469.
Sample Types: Whole Cells
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