Sheep IgG Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
IC016G
Detection of Sheep IgG Isotype Control by Flow Cytometry
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Product Details
Citations (3)
FAQs
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Sheep IgG Alexa Fluor® 488-conjugated Antibody Summary

Specificity
Serum was obtained from naive (non-immunized) sheep.
Source
Polyclonal Sheep IgG
Purification
Protein A or G purified
Immunogen
NA
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems specific polyclonal antibodies. The recommended concentration is 5 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Detection of Sheep IgG Control by Flow Cytometry C2C12 mouse myoblast cell line was stained with Sheep Anti-Mouse AHR Alexa Fluor® 488-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC6697G, filled histogram) or Sheep IgG Alexa Fluor® 488-conjugated Isotype Control Antibody (Catalog # IC016G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

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Citations for Sheep IgG Alexa Fluor® 488-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. The TLR7-IRF-5 axis sensitizes memory CD4+ T cells to Fas-mediated apoptosis during HIV-1 infection
    Authors: Carmona-Perez, L;Dagenais-Lussier, X;Mai, LT;St�gerer, T;Swaminathan, S;Isnard, S;Rice, MR;Barnes, BJ;Routy, JP;van Grevenynghe, J;Stager, S;
    JCI insight
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Profound inhibition of CD73-dependent formation of anti-inflammatory adenosine in B cells of SLE patients
    Authors: J Hesse, M Siekierka-, B Steckel, C Alter, M Schallehn, N Honke, ML Schniering, M Wippich, R Braband, M Schneider, H Surowy, D Wieczorek, J Schrader, G Pongratz
    EBioMedicine, 2021-10-16;73(0):103616.
    Applications: Flow Cytometry
  3. Multiscale cytometry and regulation of 3D cell cultures on a chip
    Authors: S Sart, RF Tomasi, G Amselem, CN Baroud
    Nat Commun, 2017-09-07;8(1):469.
    Applications: ICC

FAQs

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