Human TTK Antibody

Catalog #: AF6028 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6028
AF6028-SP

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Human TTK Antibody Summary

Species Reactivity

Human

Specificity

Detects human TTK in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human TTK
Ser281-Leu481
Accession # P33981

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human TTK by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, and MCF-7 human breast cancer cell line. PVDF Membrane was probed with 0.5 µg/mL of Sheep Anti-Human TTK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6028) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TTK at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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TTK in Human Squamous Cell Carcinoma. TTK was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Sheep Anti-Human TTK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6028) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TTK

TTK (Dual specificity Thr/Tyr kinase; also PYT and hMps1) is a 97-105 kDa member of the Ser/Thr protein kinase family of enzymes. It is expressed in rapidly proliferating tissues and cell lines, and participates in cell cycle regulation. In particular, it directs chromosome alignment, centrosome duplication, and spindle assembly checkpoint formation. Via phosphorylation, TTK is known to regulate p53, CDCA8 and TACC2. Human TTK is 857 amino acids (aa) in length and contains one protein kinase domain (aa 525-791). Activation occurs following homodimerization and autophosphorylation on Thr675, Thr676 and Thr686.

Long Name

TTK Protein Kinase

Entrez Gene IDs

7272 (Human); 22137 (Mouse); 315852 (Rat)

Alternate Names

CT96; dual specificity protein kinase TTK; EC 2.7.12.1; ESK; FLJ38280; monopolar spindle 1-like 1; Mps1; MPS1L1; MPS1L1PYTcancer/testis antigen 96; phosphotyrosine picked threonine kinase; Phosphotyrosine picked threonine-protein kinase; PYT; TTK protein kinase; TTK