Human Twist-1 Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
IC9160G-025
Detection  of Twist‑1 in A549 Human Cell Line by Flow Cytometry.
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Human Twist-1 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Twist-1 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 927403
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E.coli-derived recombinant human Twist-1
Met1-His202
Accession # Q15672
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection  of Twist‑1 in A549 Human Cell Line by Flow Cytometry. View Larger

Detection of Twist‑1 in A549 Human Cell Line by Flow Cytometry. A549 human lung carcinoma cell line was stained with Mouse Anti-Human Twist‑1 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC9160G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Twist-1

Twist-1 (Twist-related protein 1; also H-Twist and bHLHa38) is a 25‑27 kDa class B member of the bHLH transcription factor family of proteins. It is widely expressed in embryo, and select adult cells such as white adipocytes. In fat, Twist-1 induces fatty acid oxidation via PGC-1 alpha and CPT-1, and promotes MCP-1 and TNF-alpha secretion by adipocytes. In epithelium, Twist-1 dysregulation represses E-Cadherin and induces N-Cadherin expression, resulting in an epithelial-to-mesenchymal transition that can lead to cancer. Human Twist-1 is 202 amino acids (aa) in length. It contains a bHLH domain (aa 109-164) with an embedded DNA-binding motif. Twist-1 forms homodimers, and heterodimerizes with TCF3, HAND 1 and HAND 2. Two distinct mutations exist that may impact its dimerization pattern. There is one seven aa insertion after Ile135, and a second seven aa insertion after Pro139. A third unrelated variant shows a four Gly insert after Gly92. Full-length human Twist-1 shares 98% aa identity with mouse Twist-1.

Long Name
Twist Related Protein 1 [Drosophila]
Entrez Gene IDs
7291 (Human); 22160 (Mouse); 85489 (Rat)
Alternate Names
acrocephalosyndactyly 3; ACS3; B-HLH DNA binding protein; BHLHA38; bHLHa38BPES3; BPES3; Class A basic helix-loop-helix protein 38; CRS1; H-twist; H-twistblepharophimosis, epicanthus inversus and ptosis 3; SCSBPES2; twist homolog 1 (Drosophila); TWIST homolog of drosophila; TWIST; Twist1; Twist-1; twist-related protein 1

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