Twist-1 (Twist-related protein 1; also H-Twist and bHLHa38) is a 25‑27 kDa class B member of the bHLH transcription factor family of proteins. It is widely expressed in embryo, and select adult cells such as white adipocytes. In fat, Twist-1 induces fatty acid oxidation via PGC-1 alpha and CPT-1, and promotes MCP-1 and TNF-alpha secretion by adipocytes. In epithelium, Twist-1 dysregulation represses E-Cadherin and induces N-Cadherin expression, resulting in an epithelial-to-mesenchymal transition that can lead to cancer. Human Twist-1 is 202 amino acids (aa) in length. It contains a bHLH domain (aa 109-164) with an embedded DNA-binding motif. Twist-1 forms homodimers, and heterodimerizes with TCF3, HAND 1 and HAND 2. Two distinct mutations exist that may impact its dimerization pattern. There is one seven aa insertion after Ile135, and a second seven aa insertion after Pro139. A third unrelated variant shows a four Gly insert after Gly92. Full-length human Twist-1 shares 98% aa identity with mouse Twist-1.
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot, Intracellular Staining by Flow Cytometry, CyTOF-ready
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 927403
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Product Specifications
Immunogen
E.coli-derived recombinant human Twist-1
Met1-His202
Accession # Q15672
Met1-His202
Accession # Q15672
Specificity
Detects human Twist-1 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Twist‑1 Antibody
Detection of Human Twist‑1 by Western Blot.
Western blot shows lysates of PC-3 human prostate cancer cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Twist-1 Monoclonal Antibody (Catalog # MAB6230) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Twist-1 at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Twist‑1 in Human PBMCs by Flow Cytometry.
Human peripheral blood mononuclear cell (PBMCs) were stained with Mouse Anti-Human Twist-1 Monoclonal Antibody (Catalog # MAB6230, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Detection of Twist-1 by Western Blot
DS regulated the adhesion of the aggregates of the H9 cells via canonical Wnt signaling. A GSEA analyses of Wnt-associated genes enriched in the aggregates of the H9 cells treated with DS. B Gene expression analysis by qRT-PCR for Wnt-associated genes, relative gene expression represents data normalized to GADPH and expressed relative to untreated aggregates. C Immunofluorescence analysis of beta -catenin (red) on the aggregates of the H9 cells treated with or without DS; the nuclei were stained with DAPI. Scale bars = 50 μm. D The protein levels of total beta -catenin in nuclei and cytoplasm were determined by western blotting in the aggregates of the H9 cells after treatment with or without DS; the purity of nuclear and cytosolic fractions was verified using laminB1 and GAPDH, respectively. E Gene expression analysis by qRT-PCR for Wnt-targeted genes; relative gene expression represents data normalized to GADPH and expressed relative to untreated aggregates. F The protein levels of WNT7B, FZD8, total beta -catenin, LEF1, MMP3, MMP7, TWIST1, SNAI2, and E-cad were determined by western blotting in the aggregates of the H9 cells after treatment with or without DS; GAPDH was used as a loading control. Data represent the mean ± SD. *P < 0.05 and **P < 0.01 and ***P < 0.001. FZD, frizzled class receptor; LEF1, lymphoid enhancer-binding factor 1; TWIST, twist family bHLH transcription factor; SNAI, snail family transcriptional repressor; MMP, matrix metallopeptidase Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35619172), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Twist-1 by Western Blot
DS regulated the adhesion of the aggregates of the H9 cells via canonical Wnt signaling. A GSEA analyses of Wnt-associated genes enriched in the aggregates of the H9 cells treated with DS. B Gene expression analysis by qRT-PCR for Wnt-associated genes, relative gene expression represents data normalized to GADPH and expressed relative to untreated aggregates. C Immunofluorescence analysis of beta -catenin (red) on the aggregates of the H9 cells treated with or without DS; the nuclei were stained with DAPI. Scale bars = 50 μm. D The protein levels of total beta -catenin in nuclei and cytoplasm were determined by western blotting in the aggregates of the H9 cells after treatment with or without DS; the purity of nuclear and cytosolic fractions was verified using laminB1 and GAPDH, respectively. E Gene expression analysis by qRT-PCR for Wnt-targeted genes; relative gene expression represents data normalized to GADPH and expressed relative to untreated aggregates. F The protein levels of WNT7B, FZD8, total beta -catenin, LEF1, MMP3, MMP7, TWIST1, SNAI2, and E-cad were determined by western blotting in the aggregates of the H9 cells after treatment with or without DS; GAPDH was used as a loading control. Data represent the mean ± SD. *P < 0.05 and **P < 0.01 and ***P < 0.001. FZD, frizzled class receptor; LEF1, lymphoid enhancer-binding factor 1; TWIST, twist family bHLH transcription factor; SNAI, snail family transcriptional repressor; MMP, matrix metallopeptidase Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35619172), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human Twist‑1 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Sample: Human peripheral blood mononuclear cell (PBMCs) fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).
Sample: Human peripheral blood mononuclear cell (PBMCs) fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).
Western Blot
2 µg/mL
Sample: PC‑3 human prostate cancer cell line and HeLa human cervical epithelial carcinoma cell line
Sample: PC‑3 human prostate cancer cell line and HeLa human cervical epithelial carcinoma cell line
Reviewed Applications
Read 1 review rated 2 using MAB6230 in the following applications:
Flow Cytometry Panel Builder
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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Twist-1
Long Name
Twist Related Protein 1 [Drosophila]
Alternate Names
ACS3, BPES3, H-twist, Twist1
Gene Symbol
TWIST1
UniProt
Additional Twist-1 Products
Product Documents for Human Twist‑1 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Twist‑1 Antibody
For research use only
Citations for Human Twist‑1 Antibody
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Application: Western BlotSample Tested: mel505Species: HumanVerified Customer | Posted 03/05/20191:500 primary 1:1000 secondary ON incubation with primary 5 min exposure during imaging several non-specific bands
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Cellular Response to Hypoxia Protocols
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Liperfluo
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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