Human ULBP-4/RAET1E APC-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human ULBP‑4/RAET1E in direct ELISA and Western blot. In Western blot, approximately 20% cross‑reactivity with recombinant human (rh) ULBP-2 is observed and no cross-reactivity with rhULBP‑1 or rhULBP-3 is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 709116
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human ULBP‑4/RAET1E
    Accession # Q8TD07
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of ULBP‑4/RAET1E in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human ULBP‑4/RAET1E APC‑conjugated Monoclonal Antibody (Catalog # FAB6285A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: ULBP-4/RAET1E
ULBP-4 (cytomegalovirus glycoprotein UL16 binding protein 4), also called RAET1E (retinoic acid early transcript 1E), Letal (lymphocyte effector cell toxicity activating ligand) and NKG2DL4 (NKG2D ligand 4), is a 40-50 kDa member of the ULBP/RAET1 family of cell surface proteins that function as ligands for NKG2D (1‑6). While most family members are GPI-anchored, only ULBP-4/RAET1E and ULBP-5/RAET1G express a transmembrane form (1, 4, 6, 7). Human ULBP-4 mRNA encodes 263 amino acids (aa) including a 30 aa signal sequence, a 195 aa extracellular domain (ECD), a 23 aa transmembrane domain, and a 15 aa cytoplasmic sequence. A soluble 35 kDa form diverges at aa 208 and is thought to antagonize the transmembrane form (5). Other potential splice variants of 220, 227 and 280 aa are transmembrane proteins (8). Within the ECD, ULBP-4 shares 34-41% aa sequence identity with family members (1, 7). Rodent NKG2D ligands Rae-1 alpha -epsilon are, like human ULBP and MIB proteins, distantly related to MHC class I proteins, but none of the families share significant sequence identity (2, 4). Low expression of ULBP‑4 mRNA is found in normal tissues, with high expression variably reported in the small intestine (3) and skin (4). Expression is stimulated by TNF-alpha and down‑regulated by retinoic acid (3). ULBP-4 is abnormally expressed on most colon cancer and some other tumor cell lines and virus-infected peripheral blood cells (3, 6). ULBP-4 binds and costimulates NKG2D-expressing effector cells including NK cells, NKT cells, gamma δ T cells, and CD8+ alpha beta T cells, activating cytolytic activity and/or cytokine production (3, 4, 7). In some gamma δ T cells, direct ULBP-4 binding to both TCR gamma δ and NKG2D has been demonstrated (6). ULBP-4 is also thought to function as a minor histocompatibility antigen in humans (1).
  • References:
    1. Radosavljevic, M. et al. (2002) Genomics 79:114.
    2. Kondo, M. et al. (2010) Immunogenetics 62:441.
    3. Conejo-Garcia, J.R. et al. (2003) Cancer Biol. Ther. 2:446.
    4. Chalupny, N.J. et al. (2003) Biochem. Biophys. Res. Commun. 305:129.
    5. Cao, W. et al. (2007) J. Biol. Chem. 282:18922.
    6. Kong, Y. et al. (2009) Blood 114:310.
    7. Bacon, L. et al. (2004) J. Immunol. 173:1078.
    8. Cao, W. et al. (2008) Int. Immunol. 20:981.
  • Long Name:
    UL16 Binding Protein-4
  • Entrez Gene IDs:
    135250 (Human); 379043 (Mouse); 292461 (Rat)
  • Alternate Names:
    bA350J20.7; LETAL; LETALRAE-1-like transcript 4; Lymphocyte effector toxicity activation ligand; MGC125309; N2DL4; N2DL-4; NKG2D ligand 4; NKG2DL4; RAET1E; RAET1E2; retinoic acid early transcript 1ERL-4; RL-4; ULBP4; ULBP4MGC125308
Related Research Areas
Isotype Controls
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Mouse IgG2B APC-conjugated Antibody

Ctrl IC0041A 4  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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