Human Use1/UBE2Z Alexa Fluor™ Plus 680‑conjugated Antibody

Uba6-specific E2 Conjugating Enzyme 1 (Use1), also known as Ubiquitin-conjugating Enzyme E2Z (UBE2Z), is a 38 to 43 kDa member of the Ubiquitin-conjugating (E2) enzyme family that is highly expressed in human placenta, pancreas, spleen, and testis.  Use1 has an E2 catalytic core domain that contains an active site cysteine residue that is required for the formation of a thioester bond with Ubiquitin.  It functions specifically with the UBE1L2/UBA6 Ubiquitin-activating (E1) enzyme, rather than the UBE1 E1 enzyme, for Ubiquitin conjugation.  Expression is enriched in the cytoplasm, where it mediates the ubiquitination of RGS4 and RGS5 via the N-end rule proteolytic pathway. Use1 also functions with UBE1L2/UBA6 to mediate the conjugation of HLA F-associated Transcript 10 (FAT10), a Ubiquitin-like protein, and is auto-FAT10ylated in response to TNF-alpha and IFN-gamma.  Full-length human Use1 is 354 amino acids (aa) and shares 100% sequence identity with mouse and rat Use1.  A potential smaller isoform lacks aa 1-108.