Human Use1/UBE2Z Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human USE1/UBE2Z in direct ELISAs and Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Use1/UBE2Z
    Met1-Val354
    Accession # Q9H832
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Use1/UBE2Z by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line and PANC‑1 human pancreatic carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Use1/UBE2Z Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8154) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Use1/UBE2Z at approximately 39-41 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Use1/UBE2Z

Uba6-specific E2 Conjugating Enzyme 1 (Use1), also known as Ubiquitin-conjugating Enzyme E2Z (UBE2Z), is a 38 to 43 kDa member of the Ubiquitin-conjugating (E2) enzyme family that is highly expressed in human placenta, pancreas, spleen, and testis.  Use1 has an E2 catalytic core domain that contains an active site cysteine residue that is required for the formation of a thioester bond with Ubiquitin.  It functions specifically with the UBE1L2/UBA6 Ubiquitin-activating (E1) enzyme, rather than the UBE1 E1 enzyme, for Ubiquitin conjugation.  Expression is enriched in the cytoplasm, where it mediates the ubiquitination of RGS4 and RGS5 via the N-end rule proteolytic pathway. Use1 also functions with UBE1L2/UBA6 to mediate the conjugation of HLA F-associated Transcript 10 (FAT10), a Ubiquitin-like protein, and is auto-FAT10ylated in response to TNF-alpha and IFN-gamma.  Full-length human Use1 is 354 amino acids (aa) and shares 100% sequence identity with mouse and rat Use1.  A potential smaller isoform lacks aa 1-108.

  • Entrez Gene IDs:
    65264 (Human); 268470 (Mouse)
  • Alternate Names:
    MDS032; p31; protein p31; putative MAPK activating protein PM26; Putative MAPK-activating protein PM26; Q-SNARE; SLT1; SNARE-like tail-anchored protein 1 homolog; UBE2Z; unconventional SNARE in the ER 1 homolog (S. cerevisiae); Use1; USE1L; USE1-like protein; vesicle transport protein USE1
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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