Detection of Human Use1/UBE2Z by Western Blot.
Western blot shows lysates of A549 human lung carcinoma cell line and PANC‑1 human pancreatic carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Use1/UBE2Z Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8154) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Use1/UBE2Z at approximately 39-41 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Uba6-specific E2 Conjugating Enzyme 1 (Use1), also known
as Ubiquitin-conjugating Enzyme E2Z (UBE2Z), is a 38 to 43 kDa member of the
Ubiquitin-conjugating (E2) enzyme family that is highly expressed in human
placenta, pancreas, spleen, and testis.
Use1 has an E2 catalytic core domain that contains an active site
cysteine residue that is required for the formation of a thioester bond with
Ubiquitin. It functions specifically
with the UBE1L2/UBA6 Ubiquitin-activating (E1) enzyme, rather than the UBE1 E1 enzyme,
for Ubiquitin conjugation. Expression is
enriched in the cytoplasm, where it mediates the ubiquitination of RGS4 and
RGS5 via the N-end rule proteolytic pathway. Use1 also functions with
UBE1L2/UBA6 to mediate the conjugation of HLA F-associated Transcript 10
(FAT10), a Ubiquitin-like protein, and is auto-FAT10ylated in response to
TNF-alpha and IFN-gamma. Full-length
human Use1 is 354 amino acids (aa) and shares 100% sequence identity with mouse
and rat Use1. A potential smaller
isoform lacks aa 1-108.
Entrez Gene IDs:
65264 (Human); 268470 (Mouse)
MDS032; p31; protein p31; putative MAPK activating protein PM26; Putative MAPK-activating protein PM26; Q-SNARE; SLT1; SNARE-like tail-anchored protein 1 homolog; UBE2Z; unconventional SNARE in the ER 1 homolog (S. cerevisiae); Use1; USE1L; USE1-like protein; vesicle transport protein USE1
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