Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Use1/UBE2Z Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Use1/UBE2Z
Met1-Val354
Accession # Q9H832

Specificity

Detects human USE1/UBE2Z in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Use1/UBE2Z Antibody

Detection of Human Use1/UBE2Z antibody by Western Blot.

Detection of Human Use1/UBE2Z by Western Blot.

Western blot shows lysates of A549 human lung carcinoma cell line and PANC-1 human pancreatic carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Use1/UBE2Z Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8154) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Use1/UBE2Z at approximately 39-41 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Use1/UBE2Z Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: A549 human lung carcinoma cell line and PANC‑1 human pancreatic carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Use1/UBE2Z

Uba6-specific E2 Conjugating Enzyme 1 (Use1), also known as Ubiquitin-conjugating Enzyme E2Z (UBE2Z), is a 38 to 43 kDa member of the Ubiquitin-conjugating (E2) enzyme family that is highly expressed in human placenta, pancreas, spleen, and testis.  Use1 has an E2 catalytic core domain that contains an active site cysteine residue that is required for the formation of a thioester bond with Ubiquitin.  It functions specifically with the UBE1L2/UBA6 Ubiquitin-activating (E1) enzyme, rather than the UBE1 E1 enzyme, for Ubiquitin conjugation.  Expression is enriched in the cytoplasm, where it mediates the ubiquitination of RGS4 and RGS5 via the N-end rule proteolytic pathway. Use1 also functions with UBE1L2/UBA6 to mediate the conjugation of HLA F-associated Transcript 10 (FAT10), a Ubiquitin-like protein, and is auto-FAT10ylated in response to TNF-alpha and IFN-gamma.  Full-length human Use1 is 354 amino acids (aa) and shares 100% sequence identity with mouse and rat Use1.  A potential smaller isoform lacks aa 1-108.

Alternate Names

UBE2Z

Entrez Gene IDs

65264 (Human)

Gene Symbol

UBE2Z

UniProt

Q9H832 (Human)

Additional Use1/UBE2Z Products

Product Documents for Human Use1/UBE2Z Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Use1/UBE2Z Antibody

For research use only

Citations for Human Use1/UBE2Z Antibody

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