Human Uteroglobin Quantikine ELISA Kit

(1 citations)   
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Saliva (50 uL), Urine (50 uL), Human Milk (50 uL)
  • Sensitivity
    0.217 ng/mL
  • Assay Range
    0.8 - 50 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)
  • Specificity
    Natural and recombinant human Uteroglobin
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC181, Quantikine Immunoassay Control Set 665 for Human Uteroglobin - Please Inquire
Product Summary
The Quantikine Human Uteroglobin Immunoassay is a 4.5 hour solid phase ELISA designed to measure human Uteroglobin in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains E. coli-expressed recombinant human Uteroglobin and has been shown to accurately quantitate the recombinant factor. Results obtained measuring natural human Uteroglobin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Uteroglobin.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean5.215.831.7514.728.8
Standard Deviation0.210.491.160.280.922.09
CV%43.13.75.66.37.3

Saliva, Urine, Human Milk
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean5.115.431.35.215.429.9
Standard Deviation0.290.341.040.310.722.25
CV%5.72.23.364.77.5

Recovery

The recovery of Uteroglobin spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 102 99-109
EDTA Plasma (n=4) 95 85-107
Heparin Plasma (n=4) 95 86-109
Serum (n=4) 95 85-104
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Uteroglobin were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Uteroglobin Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Uteroglobin/SCGB1A1
Uteroglobin is small, secreted protein of the secretoglobin (SCGBs) family. Uteroglobin is expressed by Clara cells, the non-ciliated, non-mucous secretory cells predominant in lung bronchioles, and by other epithelia that communicate with the external environment. Uteroglob in binds to phospholipids and to several cell types, inhibiting inflammation, migration and invasion. It has been reported to bind the lipocalin-1 receptor.
    • Entrez Gene IDs
      7356 (Human); 22287 (Mouse); 25575 (Rat);
    • Alternate Names
      Blastokinin; CC10; CC10Urine protein 1; CC16; CCPBP; CCSP; CCSPCCPBP; Clara cell phospholipid-binding protein; Clara cells 10 kDa secretory protein; Clara; PCB-BP; SCGB1A1; Secretoglobin family 1A member 1; secretoglobin, family 1A, member 1 (uteroglobin); UGB; UGBUP-1; UP1; Urinary protein 1; Uteroglobin;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    10.   Aspirate and wash 4 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    1 Citations: Showing 1 - 1

    1. Club cell protein 16 and cytokeratin fragment 21-1 as early predictors of pulmonary complications in polytraumatized patients with severe chest trauma
      Authors: LL Negrin, G Halat, S Kettner, M Gregori, R Ristl, S Hajdu, T Heinz
      PLoS ONE, 2017;12(4):e0175303.
      Species: Human
      Sample Type: Serum
    ELISA Controls
    Description Application Cat# Citations Images  

    Quantikine Immunoassay Control Set 665 for Human Uteroglobin

    Ctrl QC181
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    Supplemental ELISA Products
    Description Application Cat# Citations Images  

    Quantikine ELISA Wash Buffer 1

    ELISA WA126 6
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