Human UVRAG Alexa Fluor® 647-conjugated Antibody

Catalog # Availability Size / Price Qty
Human UVRAG Alexa Fluor® 647-conjugated Antibody in Intracellular Staining by Flow Cytometry
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Product Details
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Human UVRAG Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Detects human UVRAG in direct ELISAs.
Monoclonal Mouse IgG1 Clone # 953035
Protein A or G purified from hybridoma culture supernatant
Human UVRAG synthetic peptide
Accession # Q9P2Y5
Supplied in a saline solution containing BSA and Sodium Azide.
Alexa Fluor 647


Recommended Concentration
Intracellular Staining by Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of UVRAG in Daudi Human Cell Line by Flow Cytometry. View Larger

Detection of UVRAG in Daudi Human Cell Line by Flow Cytometry. Daudi human Burkitt's lymphoma cell line was stained with Mouse Anti-Human UVRAG Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # IC9180R, filled histogram) or isotype control antibody (Catalog # IC002R, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: UVRAG

UV radiation resistance-associated gene protein, or UVRAG, is a multi-function protein of 699 amino acids involved in the regulation of cellular pathways implicated in membrane trafficking. UVRAG activates the Beclin-1-PI3KC3 complex, a critical element in the autophagy signaling pathway. Binding studies show that UVRAG and Beclin-1 directly interact via their coiled-coil domains and that Beclin-1 functions as a platform for the formation of the Bcl-2-UVRAG-PI3KC3 complex. Chromosomal aberrations involving the UVRAG gene are associated with left-right axis malformation, and mutations in the gene have been associated with colon cancer.

Long Name
UV Radiation Resistance Associated
Entrez Gene IDs
7405 (Human); 78610 (Mouse); 308846 (Rat)
Alternate Names
beclin 1 binding protein; DHTX; disrupted in heterotaxy; p63; UV radiation resistance associated gene; UV radiation resistance-associated gene protein; UVRAG; VPS38

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