Human UVRAG Antibody

Catalog # Availability Size / Price Qty
MAB9180-100
MAB9180-SP
Detection  of UVRAG in Raji Human Cell Line by Flow Cytometry.
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Human UVRAG Antibody Summary

Species Reactivity
Human
Specificity
Detects human UVRAG in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 953035
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human UVRAG synthetic peptide corresponding to sequences between amino acids 260-360
Accession # Q9P2Y5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection  of UVRAG in Raji Human Cell Line by Flow Cytometry. View Larger

Detection of UVRAG in Raji Human Cell Line by Flow Cytometry. Raji human Burkitt's lymphoma cell line was stained with Mouse Anti-Human UVRAG Monoclonal Antibody (Catalog # MAB9180, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Flow Cytometry Detection  of UVRAG in Daudi Human Cell Line by Flow Cytometry. View Larger

Detection of UVRAG in Daudi Human Cell Line by Flow Cytometry. Daudi human Burkitt's lymphoma cell line was stained with Mouse Anti-Human UVRAG Monoclonal Antibody (Catalog # MAB9180, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: UVRAG

UV radiation resistance-associated gene protein, or UVRAG, is a multi-function protein of 699 amino acids involved in the regulation of cellular pathways implicated in membrane trafficking. UVRAG activates the Beclin-1-PI3KC3 complex, a critical element in the autophagy signaling pathway. Binding studies show that UVRAG and Beclin-1 directly interact via their coiled-coil domains and that Beclin-1 functions as a platform for the formation of the Bcl-2-UVRAG-PI3KC3 complex. Chromosomal aberrations involving the UVRAG gene are associated with left-right axis malformation, and mutations in the gene have been associated with colon cancer.

Long Name
UV Radiation Resistance Associated
Entrez Gene IDs
7405 (Human); 78610 (Mouse); 308846 (Rat)
Alternate Names
beclin 1 binding protein; DHTX; disrupted in heterotaxy; p63; UV radiation resistance associated gene; UV radiation resistance-associated gene protein; UVRAG; VPS38

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Isotype Controls

Mouse IgG1 Isotype Control

Ctrl

Reconstitution Buffers

Secondary Antibodies

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