Human VEGF APC‑conjugated Antibody

R&D Systems | Catalog # IC2931A

R&D Systems
Discontinued Product
IC2931A has been discontinued. View all VEGF products.

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Intracellular Staining by Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 23410
View all VEGF Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human VEGF

Specificity

Detects human VEGF.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human VEGF APC‑conjugated Antibody

Detection of VEGF antibody in U937 Human Cell Line antibody by Flow Cytometry.

Detection of VEGF in U937 Human Cell Line by Flow Cytometry.

U937 human histiocytic lymphoma cell line either treated with 50 ng/mL PMA and 200 ng/mLCalcium Ionomycin for 16 hours (filled histogram) or untreated (open histogram) was stained with Mouse Anti-Human VEGF APC-conjugated Monoclonal Antibody (Catalog # IC2931A). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with methanol. View our protocol for Staining Intracellular Molecules.

Applications for Human VEGF APC‑conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: U937 human histiocytic lymphoma cell line treated with PMA and Calcium Ionomycin was fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with methanol

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: VEGF

Vascular Endothelial Growth Factor (VEGF or VEGF-A), also known as Vascular Permeability Factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues and a cystine knot structure. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189. Human VEGF165 is an approximately 44 kDa molecular weight homodimer sharing 88% aa sequence identity with corresponding regions of mouse and rat, 96% with porcine, 95% with canine, and 93% with feline, equine and bovine VEGF, respectively. VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR) on endothelial cells. Although VEGF affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity. VEGF165 binds the Semaphorin receptor, Neuropilin-1 and promotes complex formation with VEGF R2. VEGF is required during embryogenesis and functions to regulate the proliferation, migration, and survival of endothelial cells. In adults, VEGF functions mainly in wound healing and the female reproductive cycle. Pathologically, it is involved in tumor angiogenesis and vascular leakage. Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus. VEGF is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, Oncostatin M (OSM) and TNF-alpha.

Long Name

Vascular Endothelial Growth Factor

Alternate Names

MVCD1, VAS, Vasculotropin, VEGF-A, VEGFA, VPF

Entrez Gene IDs

7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)

Gene Symbol

VEGFA

Additional VEGF Products

Product Documents for Human VEGF APC‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human VEGF APC‑conjugated Antibody

For research use only

Citations for Human VEGF APC‑conjugated Antibody

Customer Reviews for Human VEGF APC‑conjugated Antibody

There are currently no reviews for this product. Be the first to review Human VEGF APC‑conjugated Antibody and earn rewards!

Have you used Human VEGF APC‑conjugated Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs for Human VEGF APC‑conjugated Antibody

Showing  1 - 1 of 1 FAQ Showing All

  • Q:  Is the use of a golgi blocker recommended prior to intracellular staining for secreted cytokines ? 

    A: Generally 3uM Monensin is used in conjunction with PMA/ionomycin stimulation to prevent signal loss.

Showing  1 - 1 of 1 FAQ Showing All
View all FAQs for Antibodies