VEGFR1 (vascular endothelial growth factor receptor 1; Flt-1) binds to VEGF-A, VEGF-B, and PlGF and functions in vasculogenesis, angiogenesis, lymphangiogenesis, and wound healing. It is expressed on endothelial cells, osteoblasts, placental trophoblasts, renal mesangial cells, and some hematopoietic stem cells. It can heterodimerize with VEGFR2 and modulate VEGFR2 mediated endothelial cell proliferation and vessel branching. VEGFR1 engagement on monocyte/macrophage lineage cells enhances their migration, and release of growth factors and cytokines.
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Human VEGFR1/Flt-1 Quantikine ELISA Kit
R&D Systems | Catalog # DVR100C
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Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 µL), Cell Lysates (10 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL), Saliva (100 µL)
Sensitivity
8.46 pg/mL
Assay Range
31.3-2000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva)
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Product Summary for Human VEGFR1/Flt-1 Quantikine ELISA Kit
The Quantikine® Human VEGF R1/Flt-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human VEGF R1 in cell culture supernates, cell lysates, serum, plasma, and saliva. It contains NS0-expressed recombinant human VEGF R1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human VEGF R1 showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human VEGF R1.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Human
Specificity
Natural and recombinant human VEGF R1.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Sample Values
| Sample Type | Mean (pg/mL | Range (pg/mL) |
| Serum (n=30) | 385 | 294-478 |
| EDTA Plasma (n=30) | 292 | 208-391 |
| Heparin Plasma (n=30) | 279 | 216-377 |
| Saliva (n=10) | 944 | 346-1827 |
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Saliva, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 207 | 610 | 1207 | 207 | 616 | 1205 |
| Standard Deviation | 5.16 | 14.9 | 35.6 | 13.9 | 38.9 | 74.0 |
| CV% | 2.5 | 2.4 | 2.9 | 6.7 | 6.3 | 6.1 |
Recovery for Human VEGFR1/Flt-1 Quantikine ELISA Kit
The recovery of human VEGF R1 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 99 | 87-110 |
| Cell Lysis Buffer (n=4) | 94 | 86-108 |
| EDTA Plasma (n=4) | 92 | 84-98 |
| Heparin Plasma (n=4) | 89 | 81-96 |
| Saliva (n=4) | 109 | 96-124 |
| Serum (n=4) | 104 | 93-114 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human VEGF R1 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human VEGFR1/Flt-1 Quantikine ELISA Kit
Human VEGF R1/Flt-1 Standard Curve
Human VEGF R1 Quantikine ELISA Kit Linearity Competitor Comparison
Human VEGF R1 Quantikine ELISA Kit Sensitivity Competitor Comparison
Human VEGF R1 Quantikine ELISA Kit Interference Competitor Comparison
Human VEGF R1/Flt-1 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: VEGFR1/Flt-1
Long Name
Vascular Endothelial Growth Factor Receptor 1
Alternate Names
Flt-1, FLT1, FRT, VEGF R1, VEGFR-1
Gene Symbol
FLT1
Additional VEGFR1/Flt-1 Products
Product Documents for Human VEGFR1/Flt-1 Quantikine ELISA Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human VEGFR1/Flt-1 Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Citations for Human VEGFR1/Flt-1 Quantikine ELISA Kit
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
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- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
MAPK Signaling Pathway: Mitogen Stimulation Pathway
mTOR Signaling Pathway
