• Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Tissue Homogenates (50 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
  • Sensitivity
    0.2 ng/mL
  • Assay Range
    0.6 - 40 ng/mL (Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Measures greater than or equal to 35 kDa Hyaluronan in human, mouse, rat, canine, and porcine samples
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    Interference observed with 1 or more available related molecules.
Control Available
QC104, Quantikine Immunoassay Control Set 746 for Hyaluronan - Please Inquire
Product Summary
The Quantikine Hyaluronan Immunoassay is a 4.5 hour solid-phase ELISA designed to measure at least 35 kDa Hyaluronan in human, mouse, rat, canine, and porcine cell culture supernates, tissue homogenates, serum, and plasma samples. It contains Streptococcus pyogenes-derived Hyaluronan and NS0-expressed human Aggrecan. This immunoassay has been shown to accurately quantitate Hyaluronan.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean1.534.8114.31.684.6814.98
Standard Deviation0.110.260.520.080.230.72
CV%7.25.43.64.84.94.8

Recovery

The recovery of Hyaluronan spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 104 96-116
EDTA Plasma (n=10) 97 83-106
Heparin Plasma (n=10) 99 80-124
Serum (n=10) 98 81-119
Linearity
To assess the linearity of the assay, samples containing high concentrations of Hyaluronan were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Hyaluronan Quantikine ELISA Kit
Hyaluronan Quantikine ELISA Kit
Hyaluronan Quantikine ELISA Kit
Hyaluronan Quantikine ELISA Kit
Hyaluronan Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Hyaluronan
Hyaluronan, also known as hyaluronic acid (HA) or sodium hyaluronate, is a naturally occurring linear polymer. Hyaluronan is a glycosaminoglycan (GAG) that is ubiquitously present in the extracellular matrix of all vertebrates and is also present in the capsule of some strains of Streptococci. Mammalian hyaluronan is synthesized by one of three distinct hyaluronan synthases (HAS1, 2, and 3), which produce HA polymers with different chain lengths and differ in their rates of synthesis. Whereas high molecular weight HA (> 500 kDa) is anti-angiogenic, anti-inflammatory and immunosuppressive, low molecular weight HA (10 - 500 kDa) is highly angiogenic and pro-inflammatory. HA oligomers are anti-apoptotic and upregulate heat shock protein expression. Functionally, hyaluronan molecules are important for the maintenance of a highly hydrated extracellular matrix in tissues, which is involved in cell adhesion and supports cell migration.
    • Alternate Names
      Hyaluronan;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 50 µL Assay Diluent
    4.   Add 50 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
    7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

    8. 100 µL Conjugate
    9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
    10.   Aspirate and wash 5 times.

    11. 100 µL Substrate Solution
    12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

    13. 100 µL Stop Solution
    14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    10 Citations: Showing 1 - 10
    Filter your results:

    Species
    Sample Type
    1. Anti-tumor and anti-invasion effects of a combination of 4-methylumbelliferone and ionizing radiation in human fibrosarcoma cells
      Authors: R Saga, S Monzen, M Chiba, H Yoshino, T Nakamura, Y Hosokawa
      Oncol Lett, 2017;13(1):410-416.
      Species: Human
      Sample Type: Cell Culture Supernates
    2. Umbilical cord extracts improve diabetic abnormalities in bone marrow-derived mesenchymal stem cells and increase their therapeutic effects on diabetic nephropathy
      Authors: K Nagaishi, Y Mizue, T Chikenji, M Otani, M Nakano, Y Saijo, H Tsuchida, S Ishioka, A Nishikawa, T Saito, M Fujimiya
      Sci Rep, 2017;7(1):8484.
      Species: Human
      Sample Type: Cell Culture Supernates
    3. A prognostic model for development of significant liver fibrosis in HIV-hepatitis C co-infection
      Authors: N Moqueet, C Kanagarath, MJ Gill, M Hull, S Walmsley, D Radzioch, S Saeed, RW Platt, MB Klein,
      PLoS ONE, 2017;12(5):e0176282.
      Species: Human
      Sample Type: Serum
    4. CD4+ T lymphocytes produce adiponectin in response to transplants
      Authors: S Danturti, KS Keslar, LR Steinhoff, R Fan, N Dvorina, A Valujskikh, RL Fairchild, WM Baldwin
      JCI Insight, 2017;2(12):.
      Species: Mouse
      Sample Type: Tissue Homogenates
    5. Serum levels of endothelial glycocalyx constituents in women at 20?weeks' gestation who later develop gestational diabetes mellitus compared to matched controls: a pilot study
      BMJ Open, 2016;6(12):e011244.
      Species: Human
      Sample Type: Serum
    6. Identification of aortic arch-specific quantitative trait loci for atherosclerosis by an intercross of DBA/2J and 129S6 apolipoprotein E-deficient mice.
      Authors: Kayashima Y, Makhanova N, Matsuki K, Tomita H, Bennett B, Maeda N
      PLoS ONE, 2015;10(2):e0117478.
      Species: Mouse
      Sample Type: Plasma
    7. Loss of the endothelial glycocalyx is associated with increased E-selectin mediated adhesion of lung tumour cells to the brain microvascular endothelium.
      Authors: Rai S, Nejadhamzeeigilani Z, Gutowski N, Whatmore J
      J Exp Clin Cancer Res, 2015;34(0):105.
      Species: Human
      Sample Type: Cell Culture Supernates
    8. Sustained Isoprostane E2 Elevation, Inflammation and Fibrosis after Acute Ischaemia-Reperfusion Injury Are Reduced by Pregnane X Receptor Activation.
      Authors: Amer A, Probert P, Dunn M, Knight M, Vallance A, Flecknell P, Oakley F, Cameron I, White S, Blain P, Wright M
      PLoS ONE, 2015;10(8):e0136173.
      Species: Rat
      Sample Type: Serum
    9. Endothelial glycocalyx shedding and vascular permeability in severely injured trauma patients.
      Authors: Rahbar E, Cardenas J, Baimukanova G, Usadi B, Bruhn R, Pati S, Ostrowski S, Johansson P, Holcomb J, Wade C
      J Transl Med, 2015;13(0):117.
      Species: Human
      Sample Type: Plasma
    10. Prognostic impact of hyaluronan and its regulators in pancreatic ductal adenocarcinoma.
      Authors: Cheng, Xiao-Bo, Sato, Norihiro, Kohi, Shiro, Yamaguchi, Koji
      PLoS ONE, 2013;8(11):e80765.
      Species: Human
      Sample Type: Tissue Homogenates
    ELISA Controls
    Description Application Cat# Citations Images  

    Quantikine Immunoassay Control Set 746 for Hyaluronan

    Ctrl QC104
    View Sizes & Prices
    Catalog# Size Price Stock Quantity
    Loading Information....
    Supplemental ELISA Products
    Description Application Cat# Citations Images  

    Quantikine ELISA Wash Buffer 1

    ELISA WA126 6
    View Sizes & Prices
    Catalog# Size Price Stock Quantity
    Loading Information....

    Cell Lysis Buffer 2

    ELISA 895347 2
    View Sizes & Prices
    Catalog# Size Price Stock Quantity
    Loading Information....
    Average Rating: 5 (Based on 5 reviews)

    Have you used Hyaluronan Quantikine ELISA Kit?

    Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

    Read what people are saying who have used Hyaluronan Quantikine ELISA Kit.

    Filter by Ratings
    Excellent
    (5)
    All Ratings
    Filter by Applications
    All Applications
    Filter by Species
    All Species

    Images Ratings Applications Species Reviewed By Date Details
     Hyaluronan Quantikine ELISA Kit DHYAL0
    Enlarge
    Excellent
      Anonymous 09/27/2017
    Show
    Image Details
     Hyaluronan Quantikine ELISA Kit DHYAL0
    : Hyaluronan Quantikine ELISA Kit [DHYAL0]

    Summary

    Sample TestedHuman cell conditioned medium

    Other Experimental Details

    Other Experimental DetailsVery sensitive. Great product.
     Hyaluronan Quantikine ELISA Kit DHYAL0
    Enlarge
    Excellent
      Anonymous 07/26/2017
    Show
    Image Details
     Hyaluronan Quantikine ELISA Kit DHYAL0
    : Hyaluronan Quantikine ELISA Kit [DHYAL0]

    Summary

    Sample TestedSerum

    Other Experimental Details

    Other Experimental DetailsRobust and easy to use assay
     Hyaluronan Quantikine ELISA Kit DHYAL0
    Enlarge
    Excellent
      Anonymous 05/16/2017
    Show
    Image Details
     Hyaluronan Quantikine ELISA Kit DHYAL0
    : Hyaluronan Quantikine ELISA Kit [DHYAL0]

    Summary

    Sample Testedcollagen based tissue,Adipose tissue
     Hyaluronan Quantikine ELISA Kit DHYAL0
    Enlarge
    Excellent
      Anonymous 04/24/2017
    Show
    Image Details
     Hyaluronan Quantikine ELISA Kit DHYAL0
    : Hyaluronan Quantikine ELISA Kit [DHYAL0]

    Summary

    Sample TestedBronchoalveolar lavage

    Other Experimental Details

    Other Experimental DetailsHyaluronan Quantikine ELISA kit was used to for diagnostic use.
      Excellent
      Anonymous 04/22/2017
    Show

    Summary

    Sample TestedSerum and Plasma

    Order Details

    Contact Information

    R&D Systems Guarantee

    Feedback

    Customer Information