Hyaluronan Quantikine ELISA Kit

  (15 citations)
(6 Reviews)
    
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Product Details
Assay Procedure
Citations (15)
FAQs
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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Tissue Homogenates (50 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
  • Sensitivity
    0.2 ng/mL
  • Assay Range
    0.6 - 40 ng/mL (Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Measures greater than or equal to 35 kDa Hyaluronan in human, mouse, rat, canine, and porcine samples
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    Interference observed with 1 or more available related molecules.
Control Available
QC104 , Quantikine Immunoassay Control Set 746 for Hyaluronan - Please Inquire
Product Summary
The Quantikine Hyaluronan Immunoassay is a 4.5 hour solid-phase ELISA designed to measure at least 35 kDa Hyaluronan in human, mouse, rat, canine, and porcine cell culture supernates, tissue homogenates, serum, and plasma samples. It contains Streptococcus pyogenes-derived Hyaluronan and NS0-expressed human Aggrecan. This immunoassay has been shown to accurately quantitate Hyaluronan.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 1.53 4.81 14.3 1.68 4.68 14.98
Standard Deviation 0.11 0.26 0.52 0.08 0.23 0.72
CV% 7.2 5.4 3.6 4.8 4.9 4.8

Recovery

The recovery of Hyaluronan spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 104 96-116
EDTA Plasma (n=10) 97 83-106
Heparin Plasma (n=10) 99 80-124
Serum (n=10) 98 81-119
Linearity
To assess the linearity of the assay, samples containing high concentrations of Hyaluronan were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
 Hyaluronan [HRP]
 Hyaluronan [HRP]
 Hyaluronan [HRP]
 Hyaluronan [HRP]
 Hyaluronan [HRP]
Product Datasheets

Certificate of Analysis Lookup

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Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Hyaluronan
Hyaluronan, also known as hyaluronic acid (HA) or sodium hyaluronate, is a naturally occurring linear polymer. Hyaluronan is a glycosaminoglycan (GAG) that is ubiquitously present in the extracellular matrix of all vertebrates and is also present in the capsule of some strains of Streptococci. Mammalian hyaluronan is synthesized by one of three distinct hyaluronan synthases (HAS1, 2, and 3), which produce HA polymers with different chain lengths and differ in their rates of synthesis. Whereas high molecular weight HA (> 500 kDa) is anti-angiogenic, anti-inflammatory and immunosuppressive, low molecular weight HA (10 - 500 kDa) is highly angiogenic and pro-inflammatory. HA oligomers are anti-apoptotic and upregulate heat shock protein expression. Functionally, hyaluronan molecules are important for the maintenance of a highly hydrated extracellular matrix in tissues, which is involved in cell adhesion and supports cell migration.
  • Alternate Names:
    Hyaluronan
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

  8. 100 µL Conjugate
  9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  10.   Aspirate and wash 5 times.

  11. 100 µL Substrate Solution
  12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  13. 100 µL Stop Solution
  14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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Species
Sample Type
  1. Fetal extracellular matrix nerve wraps locally improve peripheral nerve remodeling after complete transection and direct repair in rat
    Authors: T Ren, A Faust, Y van der Me, B Xiao, S Johnson, A Kandakatla, VS Gorantla, SF Badylak, KM Washington, MB Steketee
    Sci Rep, 2018;8(1):4474.
    Species: Porcine
    Sample Type: Tissue Homogenates
  2. Anti-tumor and anti-invasion effects of a combination of 4-methylumbelliferone and ionizing radiation in human fibrosarcoma cells
    Authors: R Saga, S Monzen, M Chiba, H Yoshino, T Nakamura, Y Hosokawa
    Oncol Lett, 2017;13(1):410-416.
    Species: Human
    Sample Type: Cell Culture Supernates
  3. Umbilical cord extracts improve diabetic abnormalities in bone marrow-derived mesenchymal stem cells and increase their therapeutic effects on diabetic nephropathy
    Authors: K Nagaishi, Y Mizue, T Chikenji, M Otani, M Nakano, Y Saijo, H Tsuchida, S Ishioka, A Nishikawa, T Saito, M Fujimiya
    Sci Rep, 2017;7(1):8484.
    Species: Human
    Sample Type: Cell Culture Supernates
  4. A prognostic model for development of significant liver fibrosis in HIV-hepatitis C co-infection
    Authors: N Moqueet, C Kanagarath, MJ Gill, M Hull, S Walmsley, D Radzioch, S Saeed, RW Platt, MB Klein,
    PLoS ONE, 2017;12(5):e0176282.
    Species: Human
    Sample Type: Serum
  5. CD4+ T lymphocytes produce adiponectin in response to transplants
    Authors: S Danturti, KS Keslar, LR Steinhoff, R Fan, N Dvorina, A Valujskikh, RL Fairchild, WM Baldwin
    JCI Insight, 2017;2(12):.
    Species: Mouse
    Sample Type: Tissue Homogenates
  6. Cervical HSV-2 infection causes cervical remodeling and increases risk for ascending infection and preterm birth
    Authors: D McGee, A Smith, S Poncil, A Patterson, AI Bernstein, K Racicot
    PLoS ONE, 2017;12(11):e0188645.
    Species: Human
    Sample Type: Cell Culture Supernates
  7. Up-regulation of Interleukin-21 Contributes to Liver Pathology of Schistosomiasis by Driving GC Immune Responses and Activating HSCs in Mice
    Authors: Y Wang, C Lin, Y Cao, Z Duan, Z Guan, J Xu, XQ Zhu, C Xia
    Sci Rep, 2017;7(1):16682.
    Species: Mouse
    Sample Type: Serum
  8. Cactus cladodes (Opuntia humifusa) extract minimizes the effects of UV irradiation on keratinocytes and hairless mice
    Authors: K Park, HS Choi, YH Hong, EY Jung, HJ Suh
    Pharm Biol, 2017;55(1):1032-1040.
    Species: Mouse
    Sample Type: Tissue Homogenates
  9. Serum levels of endothelial glycocalyx constituents in women at 20?weeks' gestation who later develop gestational diabetes mellitus compared to matched controls: a pilot study
    BMJ Open, 2016;6(12):e011244.
    Species: Human
    Sample Type: Serum
  10. Linking the Transcriptional Landscape of Bone Induction to Biomaterial Design Parameters
    Authors: Nathalie Groen
    Adv. Mater. Weinheim, 2016;0(0):.
    Species: Human
    Sample Type: Cell Lysates
  11. Identification of aortic arch-specific quantitative trait loci for atherosclerosis by an intercross of DBA/2J and 129S6 apolipoprotein E-deficient mice.
    Authors: Kayashima Y, Makhanova N, Matsuki K, Tomita H, Bennett B, Maeda N
    PLoS ONE, 2015;10(2):e0117478.
    Species: Mouse
    Sample Type: Plasma
  12. Loss of the endothelial glycocalyx is associated with increased E-selectin mediated adhesion of lung tumour cells to the brain microvascular endothelium.
    Authors: Rai S, Nejadhamzeeigilani Z, Gutowski N, Whatmore J
    J Exp Clin Cancer Res, 2015;34(0):105.
    Species: Human
    Sample Type: Cell Culture Supernates
  13. Sustained Isoprostane E2 Elevation, Inflammation and Fibrosis after Acute Ischaemia-Reperfusion Injury Are Reduced by Pregnane X Receptor Activation.
    Authors: Amer A, Probert P, Dunn M, Knight M, Vallance A, Flecknell P, Oakley F, Cameron I, White S, Blain P, Wright M
    PLoS ONE, 2015;10(8):e0136173.
    Species: Rat
    Sample Type: Serum
  14. Endothelial glycocalyx shedding and vascular permeability in severely injured trauma patients.
    Authors: Rahbar E, Cardenas J, Baimukanova G, Usadi B, Bruhn R, Pati S, Ostrowski S, Johansson P, Holcomb J, Wade C
    J Transl Med, 2015;13(0):117.
    Species: Human
    Sample Type: Plasma
  15. Prognostic impact of hyaluronan and its regulators in pancreatic ductal adenocarcinoma.
    Authors: Cheng, Xiao-Bo, Sato, Norihiro, Kohi, Shiro, Yamaguchi, Koji
    PLoS ONE, 2013;8(11):e80765.
    Species: Human
    Sample Type: Tissue Homogenates
Expand to show all Citations

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ELISA Controls
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Quantikine Immunoassay Control Set 746 for Hyaluronan

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Quantikine ELISA Wash Buffer 1

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Cell Lysis Buffer 2

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Average Rating: 5 (Based on 6 reviews)

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 Hyaluronan Quantikine ELISA Kit DHYAL0 Array DHYAL0
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Excellent
  Anonymous 04/23/2018
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Image Details
 Hyaluronan Quantikine ELISA Kit DHYAL0
: Hyaluronan Quantikine ELISA Kit [DHYAL0].

Summary

Sample Tested Mouse serum
 Hyaluronan Quantikine ELISA Kit DHYAL0 Array DHYAL0
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Excellent
  Anonymous 09/27/2017
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Image Details
 Hyaluronan Quantikine ELISA Kit DHYAL0
: Hyaluronan Quantikine ELISA Kit [DHYAL0].

Summary

Sample Tested Human cell conditioned medium

Other Experimental Details

Other Experimental Details Very sensitive. Great product.
 Hyaluronan Quantikine ELISA Kit DHYAL0 Array DHYAL0
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Excellent
  Anonymous 07/26/2017
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Image Details
 Hyaluronan Quantikine ELISA Kit DHYAL0
: Hyaluronan Quantikine ELISA Kit [DHYAL0].

Summary

Sample Tested Serum

Other Experimental Details

Other Experimental Details Robust and easy to use assay
 Hyaluronan Quantikine ELISA Kit DHYAL0 Array DHYAL0
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Excellent
  Anonymous 05/16/2017
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Image Details
 Hyaluronan Quantikine ELISA Kit DHYAL0
: Hyaluronan Quantikine ELISA Kit [DHYAL0].

Summary

Sample Tested collagen based tissue,Adipose tissue
 Hyaluronan Quantikine ELISA Kit DHYAL0 Array DHYAL0
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Excellent
  Anonymous 04/24/2017
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Image Details
 Hyaluronan Quantikine ELISA Kit DHYAL0
: Hyaluronan Quantikine ELISA Kit [DHYAL0].

Summary

Sample Tested Bronchoalveolar lavage

Other Experimental Details

Other Experimental Details Hyaluronan Quantikine ELISA kit was used to for diagnostic use.
  Excellent
  Anonymous 04/22/2017
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Sample Tested Serum and Plasma

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