Hyaluronan, also known as hyaluronic acid (HA) or sodium hyaluronate, is a naturally occurring linear polymer. Hyaluronan is a glycosaminoglycan (GAG) that is ubiquitously present in the extracellular matrix of all vertebrates and is also present in the capsule of some strains of Streptococci. Mammalian hyaluronan is synthesized by one of three distinct hyaluronan synthases (HAS1, 2, and 3), which produce HA polymers with different chain lengths and differ in their rates of synthesis. Whereas high molecular weight HA (> 500 kDa) is anti-angiogenic, anti-inflammatory and immunosuppressive, low molecular weight HA (10 - 500 kDa) is highly angiogenic and pro-inflammatory. HA oligomers are anti-apoptotic and upregulate heat shock protein expression. Functionally, hyaluronan molecules are important for the maintenance of a highly hydrated extracellular matrix in tissues, which is involved in cell adhesion and supports cell migration.
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Hyaluronan Quantikine ELISA Kit
R&D Systems | Catalog # DHYAL0
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Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Tissue Homogenates (50 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)
Sensitivity
0.2 ng/mL
Assay Range
0.625-40 ng/mL (Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma)
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Product Summary for Hyaluronan Quantikine ELISA Kit
The Quantikine Hyaluronan Immunoassay is a 4.5 hour solid-phase ELISA designed to measure at least 35 kDa Hyaluronan in human, mouse, rat, canine, and porcine cell culture supernates, tissue homogenates, serum, and plasma samples. It contains Streptococcus pyogenes-derived Hyaluronan and NS0-expressed human Aggrecan. This immunoassay has been shown to accurately quantitate Hyaluronan.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Multi-Species
Specificity
Measures greater than or equal to 35 kDa Hyaluronan in human, mouse, rat, canine, and porcine samples
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Tissue Homogenates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 1.53 | 4.81 | 14.3 | 1.68 | 4.68 | 14.98 |
| Standard Deviation | 0.11 | 0.26 | 0.52 | 0.08 | 0.23 | 0.72 |
| CV% | 7.2 | 5.4 | 3.6 | 4.8 | 4.9 | 4.8 |
Recovery for Hyaluronan Quantikine ELISA Kit
The recovery of Hyaluronan spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 104 | 96-116 |
| EDTA Plasma (n=10) | 97 | 83-106 |
| Heparin Plasma (n=10) | 99 | 80-124 |
| Serum (n=10) | 98 | 81-119 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of Hyaluronan were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Hyaluronan Quantikine ELISA Kit
Human, Mouse, Rat, Porcine, Canine Hyaluronan ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Hyaluronan
Additional Hyaluronan Products
Product Documents for Hyaluronan Quantikine ELISA Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Hyaluronan Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Related Research Areas
Citations for Hyaluronan Quantikine ELISA Kit
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Customer Reviews for Hyaluronan Quantikine ELISA Kit (14)
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14 Customer Ratings
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Sample Tested: Platelet-poor EDTA PlasmaVerified Customer | Posted 07/14/2022
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Sample Tested: Serum-free Cell Culture MediaVerified Customer | Posted 06/20/2022
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Sample Tested: 4T1 mouse breast cancer cell lineVerified Customer | Posted 04/16/2022
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Sample Tested: MDA-MB-231 human breast cancer cell lineVerified Customer | Posted 04/16/2022
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Sample Tested: Human plasmas and Plasmas from mice fed with HFDVerified Customer | Posted 01/08/2022Excellent product. Unlike other kits, I just needed 10 ul of plasmas for my analysis.
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Sample Tested: human floxp embryonal stem cell culture mediumVerified Customer | Posted 11/22/2021
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Sample Tested: Heart tissue and L-929 mouse fibroblast cell lineVerified Customer | Posted 10/20/2020
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Sample Tested: SerumVerified Customer | Posted 07/17/2020
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Sample Tested: mouse serumVerified Customer | Posted 04/23/2018
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Sample Tested: Human cell conditioned mediumVerified Customer | Posted 09/27/2017Very sensitive. Great product.
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Sample Tested: SerumVerified Customer | Posted 07/26/2017Robust and easy to use assay
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Sample Tested: collagen based tissue and Adipose tissueVerified Customer | Posted 05/16/2017
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Sample Tested: Bronchoalveolar lavageVerified Customer | Posted 04/24/2017Hyaluronan Quantikine ELISA kit was used to for diagnostic use.
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Sample Tested: Serum and PlasmaVerified Customer | Posted 04/22/2017
There are no reviews that match your criteria.
Protocols
View specific protocols for Hyaluronan Quantikine ELISA Kit (DHYAL0):
Refer to the product for complete assay procedure.
Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
- Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
- Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
- Aspirate and wash 5 times.
- Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
- Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
50 µL Assay Diluent
50 µL Standard, Control, or Sample
100 µL Conjugate
100 µL Substrate Solution
100 µL Stop Solution
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Hyaluronan Quantikine ELISA Kit
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Q: Assay Diluent RD1-14 contains visible precipitate. Is this acceptable to use?
A: A small amount of precipitate is expected and acceptable in Assay Diluent RD1-14. Variability has been observed between vials and diluent lots. It may not fully resolubilize but this will not impact assay performance. It is not recommended to filter the precipitate.