I-BET 151 dihydrochloride

Tocris Bioscience | Catalog # 4650

BET bromodomain inhibitor; also promotes differentiation of hiPSCs into megakaryocytes
Tocris Bioscience

Key Product Details

Description

BET bromodomain inhibitor; also promotes differentiation of hiPSCs into megakaryocytes
View all Bromodomain Inhibitors »

Product Description

I-BET 151 dihydrochloride is a BET bromodomain inhibitor; blocks recruitment of BET to chromatin. Induces apoptosis and G0/G1 cell cycle arrest in MLL-fusion leukemic cell lines in vitro (IC50 values are 15, 26, 120 and 192 nM for NOMO1, MV4;11, MOLM13 and RS4;11 cell lines respectively); reduces BCL2 expression in NOMO1 cells. Improves survival in two rodent models of MLL-fusion leukemia in vivo. Enhances differentiation of human iPSC into megakaryocytes. Also enhances fibroblast reprogramming to hiPSCs at low concentration. Used in protocol to differentiate fibroblasts directly into neurons (see below).

For more information about how I-BET 151 dihydrochloride may be used, see our protocol: Transdifferentiating Fibroblasts into Neurons.

Licensing Information

Sold for research purposes under agreement from GlaxoSmithKline

External Portal Information

Chemicalprobes.org is a portal that offers independent guidance on the selection and/or application of small molecules for research. The use of I-BET 151 is reviewed on the chemical probes website.

Product Specifications for I-BET 151 dihydrochloride

Molecular Weight

488.37

Formula

C23H21N5O3.2HCl

Storage

Store at -20°C

Purity

≥98% (HPLC)

Chemical Name

7-(3,5-Dimethyl-4-isoxazolyl)-1,3-dihydroxy-8-methoxy-1-[(1R)-1-(2-pyridinyl)ethyl]-2H-imidazo[4,5-c]quinolin-2-one dihydrochloride

CAS Number

1883545-47-8

PubChem ID

170320

InChI Key

IQOJZZHRYSSFJM-UHFFFAOYSA-N

SMILES

O=C(N3[C@H](C)C4=NC=CC=C4)NC2=C3C1=CC(OC)=C(C5=C(C)ON=C5C)C=C1N=C2.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 48.84 100
DMSO 41.54 100
Ethanol 41.54 100

Preparing Stock Solutions for I-BET 151 dihydrochloride

The following data is based on the product molecular weight 488.37.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.05 mL 10.24 mL 20.48 mL
5 mM 0.41 mL 2.05 mL 4.10 mL
10 mM 0.20 mL 1.02 mL 2.05 mL
50 mM 0.04 mL 0.20 mL 0.41 mL

Calculators

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 9 publications citing the usage of this product.

Product Documents for I-BET 151 dihydrochloride

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for I-BET 151 dihydrochloride

For research use only

Citations for I-BET 151 dihydrochloride

Customer Reviews for I-BET 151 dihydrochloride (2)

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  • Good
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: U2OS
    Verified Customer | Posted 06/22/2020
    I used short treatments with I-BET151 in the presence of JQ1.
    I-BET151 (1 μM)
    I-BET 151 dihydrochloride 4650
  • Reprogramming media with i-BET
    Name: Anonymous
    Species: Mouse
    Assay Type: In Vitro
    Cell Line/Tissue: ipsc
    Verified Customer | Posted 02/23/2020
    100 nM
    I-BET 151 dihydrochloride 4650

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