IBRDC2 Antibody (4F1) - Azide and BSA Free

Novus Biologicals | Catalog # H00255488-M02

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4F1

Format

Azide and BSA Free
View all IBRDC2 Primary Antibodies »

Product Specifications

Immunogen

IBRDC2 (NP_877434, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGSAGRLHYLAMTAENPTPGDLAPAPLITCKLCLCEQSLDKMTTLQECQCIFCTACLKQYMQLAIREGCGSPITCPDMVCLNHGTLQEAEIACLVPVDQF

Reactivity Notes

Other species not tested.

Specificity

IBRDC2 (4F1)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for IBRDC2 Antibody (4F1) - Azide and BSA Free

Western Blot: IBRDC2 Antibody (4F1) [H00255488-M02]

Western Blot: IBRDC2 Antibody (4F1) [H00255488-M02]

Western Blot: IBRDC2 Antibody (4F1) [H00255488-M02] - IBRDC2 monoclonal antibody (M02), clone 4F1 Analysis of IBRDC2 expression in IMR-32.
Immunocytochemistry/ Immunofluorescence: IBRDC2 Antibody (4F1) [H00255488-M02]

Immunocytochemistry/ Immunofluorescence: IBRDC2 Antibody (4F1) [H00255488-M02]

Immunocytochemistry/Immunofluorescence: IBRDC2 Antibody (4F1) [H00255488-M02] - Analysis of monoclonal antibody to IBRDC2 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: IBRDC2 Antibody (4F1) [H00255488-M02]

Immunohistochemistry-Paraffin: IBRDC2 Antibody (4F1) [H00255488-M02]

Immunohistochemistry-Paraffin: IBRDC2 Antibody (4F1) [H00255488-M02] - Analysis of monoclonal antibody to IBRDC2 on formalin-fixed paraffin-embedded human cerebellum. Antibody concentration 1.2 ug/ml.
Western Blot: IBRDC2 Antibody (4F1) [H00255488-M02]

Western Blot: IBRDC2 Antibody (4F1) [H00255488-M02]

Western Blot: IBRDC2 Antibody (4F1) [H00255488-M02] - Analysis of RNF144B expression in transfected 293T cell line by IBRDC2 monoclonal antibody (M02), clone 4F1.Lane 1: RNF144B transfected lysate(33.6 KDa).Lane 2: Non-transfected lysate.

Applications for IBRDC2 Antibody (4F1) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate for Western Blot. Has also been used for immunofluorescence, immunohistochemistry (paraffin) and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: IBRDC2

E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, therebypromoting their degradation. Induces apoptosis via a TP53/p53-dependent but caspase-independent mechanism

Alternate Names

bA528A10.3, E3 ubiquitin-protein ligase RNF144B, EC 6.3.2, EC 6.3.2.-, IBR domain containing 2, IBR domain-containing protein 2, IBRDC2, KIAA0161, p53-inducible RING finger protein, p53RFP, PIR2, ring finger protein 144BMGC71786

Entrez Gene IDs

255488 (Human)

Gene Symbol

RNF144B

UniProt

NP_877434 (Human)

Additional IBRDC2 Products

Product Documents for IBRDC2 Antibody (4F1) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for IBRDC2 Antibody (4F1) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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