IBSP/Sialoprotein II Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-70422

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all IBSP/Sialoprotein II Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptides corresponding to IBSP (integrin-binding sialoprotein (bone sialoprotein, bone sialoprotein II)) The peptide sequence was selected from the N terminal of IBSP. Peptide sequence SATTLGYGEDATPGTGYTGLAAIQLPKKAGDITNKATKEKESDEEEEEEE The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for IBSP/Sialoprotein II Antibody - BSA Free

Western Blot: IBSP/Sialoprotein II Antibody [NBP1-70422]

Western Blot: IBSP/Sialoprotein II Antibody [NBP1-70422]

Western Blot: Sialoprotein II Antibody [NBP1-70422] - Titration: 1.25ug/ml, Positive Control: HepG2 cell lysate.

Applications for IBSP/Sialoprotein II Antibody - BSA Free

Application
Recommended Usage

Western Blot

1.0 ug/ml

Reviewed Applications

Read 1 review rated 1 using NBP1-70422 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 2% Sucrose

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IBSP/Sialoprotein II

The protein encoded by the IBSP gene is a major structural protein of the bone matrix. It constitutes approximately 12% of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor.

Long Name

Integrin-binding Sialoprotein

Alternate Names

BNSP, BSP-II, SP-II

Gene Symbol

IBSP

Additional IBSP/Sialoprotein II Products

Product Documents for IBSP/Sialoprotein II Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for IBSP/Sialoprotein II Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for IBSP/Sialoprotein II Antibody - BSA Free (1)

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  • IBSP/Sialoprotein II Antibody
    Name: Catrina Alarcon
    Application: Immunohistochemistry-Frozen
    Sample Tested: Mouse long bone and knee joint and Mouse proximal tibia
    Species: Mouse
    Verified Customer | Posted 02/03/2017
    This is the image of BSPII staining of 14 day old mouse proximal tibia. BSPII is detected by green secondary antibody, however, the stain did not work. Likely due to the fact that this antibody can only be used in humans and possibly only for WB.
    Our previous studies have shown that BSPII has high expression in the epiphysis of the developing mouse proximal tibia and distal femur. To visualize this, we stained the tissue with BSPII antibody (NBP1-70422). As it was tested to work in human tissue and with western blots and the fact that there were not many other antibody options with Novus, we attempted the same with mouse tissue. We tested both 1:50 and 1:100 concentrations due to the higher concentrations typically needed when performing IHC vs Western blot. Unfortunately our stains came up negative/unspecific. This data was controlled for by another BSPII antibody we obtained from another vendor years ago.
    IBSP/Sialoprotein II Antibody - BSA Free NBP1-70422

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