IDH2 Antibody (673) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-43572

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (96%), Chicken (95%), Porcine (96%), Rhesus Macaque (100%), Xenopus (91%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 673

Format

Azide and BSA Free
View all IDH2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human IDH2. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (91%), Zebrafish (89%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IDH2 Antibody (673) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: IDH2 Antibody (673) [NBP2-43572]

Immunocytochemistry/ Immunofluorescence: IDH2 Antibody (673) [NBP2-43572]

Immunocytochemistry/Immunofluorescence: IDH2 Antibody (673) [NBP2-43572] - HeLa cells were fixed in 4% paraformaldehyde at RT for 10 min. Green: IDH2 protein stained by IDH2 antibody [GT673] diluted at 1:100.Blue: Hoechst 33342 staining.Scale bar = 10 um.
Western Blot: IDH2 Antibody (673) [NBP2-43572]

Western Blot: IDH2 Antibody (673) [NBP2-43572]

Western Blot: IDH2 Antibody (673) [NBP2-43572] - Analysis of A. 30 ug Jurkat whole cell lysate/extract B. 30 ug Raji whole cell lysate/extract C. 30 ug K562 whole cell lysate/extract D. 30 ug THP-1 whole cell lysate/extract E. 30 ug NCI-H929 whole cell lysate/extract 10 % SDS-PAGE IDH2 antibody dilution: 1:1000
Western Blot: IDH2 Antibody (673) [NBP2-43572]

Western Blot: IDH2 Antibody (673) [NBP2-43572]

Western Blot: IDH2 Antibody (673) [NBP2-43572] - Analysis of A. 50 ug mouse brain lysate/extract 10 % SDS-PAGE IDH2 antibody [673] dilution: 1:1000.
Western Blot: IDH2 Antibody (673) [NBP2-43572]

Western Blot: IDH2 Antibody (673) [NBP2-43572]

Western Blot: IDH2 Antibody (673) [NBP2-43572] - Analysis of A. 50 ug rat brain lysate/extract 12 % SDS-PAGE IDH2 antibody [673] dilution: 1:1000
Immunocytochemistry/ Immunofluorescence: IDH2 Antibody (673) [NBP2-43572]

Immunocytochemistry/ Immunofluorescence: IDH2 Antibody (673) [NBP2-43572]

Immunocytochemistry/Immunofluorescence: IDH2 Antibody (673) [NBP2-43572] - Analysis of HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: IDH2 protein stained by IDH2 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: IDH2 Antibody (673) [NBP2-43572]

Immunohistochemistry-Paraffin: IDH2 Antibody (673) [NBP2-43572]

Immunohistochemistry-Paraffin: IDH2 Antibody (673) [NBP2-43572] - Rat duodenum [673] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
IDH2 Antibody (673) - Azide and BSA Free

Western Blot: IDH2 Antibody (673) [NBP2-43572] -

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IDH2 antibody [GT673] (NBP2-43572) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
IDH2 Antibody (673) - Azide and BSA Free

Western Blot: IDH2 Antibody (673) [NBP2-43572] -

Non-transfected (-) and transfected (+) HepG2 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH2 antibody [GT673] (NBP2-43572) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
IDH2 Antibody (673) - Azide and BSA Free

Immunohistochemistry-Paraffin: IDH2 Antibody (673) [NBP2-43572] -

IDH2 antibody [GT673] detects IDH2 protein at cytoplasm in mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
IDH2 antibody [GT673] (NBP2-43572) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for IDH2 Antibody (673) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: IDH2

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex.

Alternate Names

D2HGA2, EC 1.1.1.42, ICD-M, IDH, IDHM, IDP, IDPM, isocitrate dehydrogenase [NADP], mitochondrial, isocitrate dehydrogenase 2 (NADP+), mitochondrial, mNADP-IDH, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase

Gene Symbol

IDH2

Additional IDH2 Products

Product Documents for IDH2 Antibody (673) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for IDH2 Antibody (673) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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