Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.
Mouse IFN-alpha - Small Sample ELISA Kit (Colorimetric)
Novus Biologicals | Catalog # NBP3-42621
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Key Product Details
Sample Type & Volume Required Per Well
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids (15 - 25 uL)
Sensitivity
5.9 pg/mL (example only; lot dependent)
Assay Range
15.6 - 1000 pg/mL (example only; lot dependent)
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Product Specifications
Assay Type
Sandwich ELISA
Kit Type
ELISA Kit (Colorimetric)
Species
Mouse
Description
Assay time: 3 hours
Precision
Intra-Assay Precision (Precision within an assay) %CV 10 (example only; lot dependent)
Inter-Assay Precision (Precision between assays) %CV 12 (example only; lot dependent)
Recovery for Mouse IFN-alpha - Small Sample ELISA Kit (Colorimetric)
Recovery
78 - 91% (example only; lot dependent)
Kit Contents for Mouse IFN-alpha - Small Sample ELISA Kit (Colorimetric)
- Assay Diluent A
- Assay Diluent B
- Detection Reagent A
- Detection Reagent B
- Instruction manual
- Plate sealer for 96 wells
- Pre-coated ready to use 96-well strip plate
- Standard
- Standard Diluent
- Stop Solution
- TMB Substrate
- Wash Buffer (30 x concentrate)
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: IFN-alpha
Long Name
Interferon alpha
Alternate Names
IFNalpha, LeIF D
Gene Symbol
IFNA2
Additional IFN-alpha Products
Product Documents for Mouse IFN-alpha - Small Sample ELISA Kit (Colorimetric)
Product Specific Notices for Mouse IFN-alpha - Small Sample ELISA Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Mouse IFN-alpha - Small Sample ELISA Kit (Colorimetric)
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A: PBL states that the endotoxin level in their IFN-alpha products is <1EU/µg.
