IFT122 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87014

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (91%), Rat (91%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all IFT122 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ITKQADWARNIKEPKAAVEMYISAGEHVKAIEICGDHGWVDMLIDIARKLDKAEREPLLLCATYLKKLDSPGYAAETYLKMGDLK

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23435261)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IFT122 Antibody - BSA Free

IFT122 Antibody - BSA Free Western Blot: IFT122 Antibody - BSA Free [NBP1-87014]

Western Blot: IFT122 Antibody - BSA Free [NBP1-87014]

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp
IFT122 Antibody - BSA Free Immunohistochemistry-Paraffin: IFT122 Antibody - BSA Free [NBP1-87014]

Immunohistochemistry-Paraffin: IFT122 Antibody - BSA Free [NBP1-87014]

Staining of human stomach shows strong cytoplasmic positivity in glandular cells.

Applications for IFT122 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IFT122

IFT122 is a gene that codes for a protein necessary for cilia formation during neuronal patterning, as it recruits TULP3 to primary cilia and works as a negative regulator of Shh signaling. IFT122 has five isoforms, with lengths of 1241, 1242, 1182, 1131, and 1292 amino acids and weights of 142, 142, 135, 129, and 147 kDa respectively. Current studies are being done on several diseases and disorders including Japanese spotted fever, endemic typhus, vasomer rhinitis, trigeminal neuralgia, synostosis, brachydactyly, paraplegia, neuronitis, and hepatitis. IFT122 has also been shown to have interactions with UBC, IQCB1, and ORF.

Alternate Names

intraflagellar transport 122 homolog (Chlamydomonas), intraflagellar transport protein 122 homolog, SPGWD repeat-containing protein 140, WD repeat domain 10, WD repeat-containing protein 10, WDR10WDR10p, WDR140CED

Gene Symbol

IFT122

Additional IFT122 Products

Product Documents for IFT122 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for IFT122 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for IFT122 Antibody - BSA Free

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Protocols

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