IgE Antibody (4H10) - BSA Free

Novus Biologicals | Catalog # NB500-472

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry, Flow (Cell Surface)

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4H10

Format

BSA Free
View all IgE Primary Antibodies »

Product Specifications

Immunogen

Purified human IgE.

Specificity

The antibody 4H10 reacts with human IgE; it recognizes an epitope different from the ones recognized by BE5 and 4G7 antibodies to IgE.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for IgE Antibody (4H10) - BSA Free

Flow (Cell Surface): IgE Antibody (4H10) - BSA Free [NB500-472]

Flow (Cell Surface): IgE Antibody (4H10) - BSA Free [NB500-472]

Flow (Cell Surface): IgE Antibody (4H10) [NB500-472] - Surface staining of IgE on human peripheral blood cells with anti-IgE (4H10) purified, GAM-APC.
Flow Cytometry: IgE Antibody (4H10) - BSA Free [NB500-472]

Flow Cytometry: IgE Antibody (4H10) - BSA Free [NB500-472]

Flow Cytometry: IgE Antibody (4H10) [NB500-472] - Staining of IgE on human peripheral blood cells with anti-IgE (4H10) FITC.

Applications for IgE Antibody (4H10) - BSA Free

Application
Recommended Usage

ELISA

1:2000

Flow Cytometry

4 ug/ml
Application Notes
Purity is > 95% (by SDS-PAGE). This antibody recognizes an epitope different from the one recognized by ab773 and ab775.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: IgE

IgE is typically the least abundant isotype - blood serum IgE levels in a normal ("non-atopic") individual are ~150 ng/ml, compared to 10 mg/ml for the IgGs - it is capable of triggering the most powerful immune reactions. Most of our knowledge of IgE has come from research into the mechanism of a form of allergy known as type 1 hypersensitivity.
There is much speculation into what physiological benefits IgE contributes, and so far, circumstantial evidence in animal models and statistical population trends have hinted that IgE may be beneficial in fighting gut parasites such as Schistosoma mansoni, but this has not been conclusively proven in humans.
IgE may play an important role in the immune system's recognition of cancer, in which the stimulation of a strong cytotoxic response against cells displaying only small amounts of early cancer markers would be beneficial. IgE may be an important target in treatments for allergy and asthma.

Long Name

Immunoglobulin E

Alternate Names

IgE, immunoglobulin heavy constant epsilon

Entrez Gene IDs

3497 (Human)

Gene Symbol

IGHE

Additional IgE Products

Product Documents for IgE Antibody (4H10) - BSA Free

Certificate of Analysis

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Product Specific Notices for IgE Antibody (4H10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

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Protocols

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