Key Product Details

Species Reactivity

Chicken

Applications

Western Blot, ELISA

Label

HRP

Antibody Source

Polyclonal Rabbit IgG
View all IgG Fc Secondary Antibodies »

Product Specifications

Immunogen

Chicken IgG F(c) fragment

Specificity

No reaction was observed against Chicken IgG F(ab).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Chicken IgG, Chicken IgG F(c) and Chicken Serum

Scientific Data Images for Rabbit anti-Chicken IgG Fc Secondary Antibody [HRP]

Western Blot: Rabbit anti-Chicken IgG Fc Secondary Antibody [HRP] [NBP1-73586] - Lane 1: Chicken IgG whole molecule. Lane 2: Chicken IgG F(c) Fragment. Lane 3: Chicken IgG Fab Fragment. Lane 4: Chicken IgM Whole Molecule. Lane 5: Chicken Serum. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Chicken IgG F(c) (RABBIT) Antibody 1:2,000 for 60 min at RT. Secondary antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgG and Serum, 25 kDa for F(c) and Fab, 75 kDa for IgM. Chicken F(c) migrates slightly higher.
Rabbit anti-Chicken IgG Fc Secondary Antibody [HRP]

Rabbit anti-Chicken IgG Fc Secondary Antibody [HRP]

Western Blot of Rabbit anti-Chicken IgG Fc Secondary Antibody [HRP]

Applications for Rabbit anti-Chicken IgG Fc Secondary Antibody [HRP]

Application
Recommended Usage

ELISA

1:10000 - 1:50000

Western Blot

1:1000 - 1:10000
Application Notes
This product has been tested in ELISA and Western Blot. This product has been assayed against 1.0 ug of Chicken IgG in a standard capture ELISA using TMB as a substrate for 30 minutes at room temperature. A working dilution of 1:5,000 to 1:20,000 is suggested for this product. NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

Reconstitute with 1.0 ml deionized water (or equivalent).

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.01% Gentamicin Sulfate

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Product Documents for Rabbit anti-Chicken IgG Fc Secondary Antibody [HRP]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Rabbit anti-Chicken IgG Fc Secondary Antibody [HRP]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

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